Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,219,144	G→T	S113* (TCG→TAG)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,219,144	0	G	T	100.0%	296.6 / NA	84	S113* (TCG→TAG)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/45);  total (39/45)

CCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCG  >  NC_000913/2219009‑2219280                                                                                                                                        |                                                                                                                                         ccgccCTCTTGTAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATc                                                                                                                                      >  2:1145126/1‑140 (MQ=255) ccgccCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATc                                                                                                                                      >  2:195130/1‑140 (MQ=255) ccgccCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATc                                                                                                                                      >  2:1356019/1‑140 (MQ=255) ccgccATCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGTCGATAGGCTAGGTGTTATTTGCAGGCGCGAGCGCCAGCCAGATTAACTTGATGTGCTCCTAAtt                                                                                                                                      >  2:153534/1‑139 (MQ=255)  cgccCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCg                                                                                                                                     <  2:1402989/140‑1 (MQ=255)              gATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGTTCCTAATCGAGCTTTTTGACt                                                                                                                         <  2:463362/140‑1 (MQ=255)              gATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACt                                                                                                                         <  1:195130/140‑1 (MQ=255)              gATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACt                                                                                                                         <  2:37627/140‑1 (MQ=255)              gATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACt                                                                                                                         <  2:443166/140‑1 (MQ=255)                   cGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTtctc                                                                                                                    >  2:31575/1‑140 (MQ=255)                    gACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCg                                                                                                                   <  2:460023/140‑1 (MQ=255)                      cTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTa                                                                                                                 >  1:731072/1‑140 (MQ=255)                      cTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTa                                                                                                                 >  1:924613/1‑140 (MQ=255)                      cTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTa                                                                                                                 >  2:333022/1‑140 (MQ=255)                      cTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTa                                                                                                                 >  1:290148/1‑140 (MQ=255)                         aGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACt                                                                                                                         <  1:1542776/129‑1 (MQ=255)                         aGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACt                                                                                                                         >  2:1542776/1‑129 (MQ=255)                         aGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAAcc                                                                                                              >  1:1260535/1‑140 (MQ=255)                         aGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAAcc                                                                                                              >  2:1368434/1‑140 (MQ=255)                         aGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAAcc                                                                                                              >  2:303361/1‑140 (MQ=255)                            tCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTtg                                                                                                           <  2:69630/140‑1 (MQ=255)                              ggCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTtgct                                                                                                         >  2:1037672/1‑140 (MQ=255)                              ggCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTtgct                                                                                                         >  1:159063/1‑140 (MQ=255)                               gCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTtgctg                                                                                                        <  2:290148/140‑1 (MQ=255)                                   gCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGcgcg                                                                                                    >  1:1062443/1‑140 (MQ=255)                                   gCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGcgcg                                                                                                    <  2:1081508/140‑1 (MQ=255)                                         tGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTc                                                                                              <  1:1165525/140‑1 (MQ=255)                                          gAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTcc                                                                                             <  1:1368434/140‑1 (MQ=255)                                             tttATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACg                                                                                          <  1:1145126/140‑1 (MQ=255)                                             tttATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACg                                                                                          <  2:731072/140‑1 (MQ=255)                                             tttATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACg                                                                                          >  2:650408/1‑140 (MQ=255)                                               tATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCt                                                                                        <  2:1062443/140‑1 (MQ=255)                                                 tttttCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCa                                                                                            <  1:505377/134‑1 (MQ=255)                                                 tttttCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCa                                                                                            >  2:505377/1‑134 (MQ=255)                                                  ttttCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGGCTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCa                                                                                     <  2:873772/140‑1 (MQ=255)                                                  ttttCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCa                                                                                     <  2:924613/140‑1 (MQ=255)                                                  ttttCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCATCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCa                                                                                     <  2:139025/140‑1 (MQ=255)                                                     tctcTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGGCTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCg                                                                                  >  1:539123/1‑140 (MQ=255)                                                     tctcTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCg                                                                                  >  1:892037/1‑140 (MQ=255)                                                      ctctGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCa                                                                                            >  1:566823/1‑129 (MQ=255)                                                      ctctGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCa                                                                                            <  2:566823/129‑1 (MQ=255)                                                         tGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGtttt                                                                              <  1:919222/140‑1 (MQ=255)                                                          gCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTc                                                                             <  1:333022/140‑1 (MQ=255)                                                                         aCGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTa                                                                      <  1:167725/132‑1 (MQ=255)                                                                         aCGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTa                                                                      >  2:167725/1‑132 (MQ=255)                                                                          cGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGaaa                                                             >  2:890301/1‑140 (MQ=255)                                                                          cGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGaaa                                                             >  2:416910/1‑140 (MQ=255)                                                                           ggCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAg                                                            >  2:238959/1‑140 (MQ=255)                                                                           ggCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAg                                                            >  1:371086/1‑140 (MQ=255)                                                                           ggCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAg                                                            >  1:1218902/1‑140 (MQ=255)                                                                            gCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAgc                                                           <  2:266194/140‑1 (MQ=255)                                                                                 aGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGGGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCAt                                                      >  2:1492084/1‑140 (MQ=255)                                                                                 aGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCAt                                                      <  2:1218902/140‑1 (MQ=255)                                                                                  gTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCAtt                                                     <  2:1419294/140‑1 (MQ=255)                                                                                  gTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCAtt                                                     >  1:1419294/1‑140 (MQ=255)                                                                                    ccAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGc                                                   <  1:714894/140‑1 (MQ=255)                                                                                        gtgtTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               <  2:892037/140‑1 (MQ=255)                                                                                        gtgtTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               <  1:31575/140‑1 (MQ=255)                                                                                        gtgtTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               <  1:303361/140‑1 (MQ=255)                                                                                        gtgtTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               <  1:1037672/140‑1 (MQ=255)                                                                                           ttATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTg                                                    <  2:1567037/132‑1 (MQ=255)                                                                                           ttATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTg                                                    >  1:1567037/1‑132 (MQ=255)                                                                                           ttATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGtata                                            >  1:1437168/1‑140 (MQ=255)                                                                                           ttATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGtata                                            >  1:1147092/1‑140 (MQ=255)                                                                                             gtttCCATGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATAtt                                          <  1:153534/139‑1 (MQ=255)                                                                                              tttGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTc                                         <  1:890301/140‑1 (MQ=255)                                                                                                  cAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGga                                     <  2:159063/140‑1 (MQ=255)                                                                                                  cAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGga                                     <  1:1468595/140‑1 (MQ=255)                                                                                                  cAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGga                                     <  2:1471503/140‑1 (MQ=255)                                                                                                   aGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGgat                                    <  1:238959/140‑1 (MQ=255)                                                                                                     gcgcGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               >  1:962125/1‑127 (MQ=255)                                                                                                     gcgcGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGt                                               <  2:962125/127‑1 (MQ=255)                                                                                                     gcgcGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGgatag                                  >  2:1504396/1‑140 (MQ=255)                                                                                                     gcgcGGGCGTCAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGgatag                                  <  2:1437168/140‑1 (MQ=255)                                                                                                               cAGCCAGATTAACTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGtt                        <  1:650408/140‑1 (MQ=255)                                                                                                                          aCTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTa             >  1:1038227/1‑140 (MQ=255)                                                                                                                           cTTGTTGTGCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTaa            <  1:1356019/140‑1 (MQ=255)                                                                                                                                tgtgCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGcc       <  2:1038227/140‑1 (MQ=255)                                                                                                                                tgtgCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAATAATCGcc       >  1:1125312/1‑140 (MQ=255)                                                                                                                                 gtgCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGccc      >  1:1215909/1‑140 (MQ=255)                                                                                                                                   gCTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCcg    <  2:371086/140‑1 (MQ=255)                                                                                                                                    cTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCcgc   >  1:903375/1‑140 (MQ=255)                                                                                                                                    cTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCcgc   >  2:898126/1‑140 (MQ=255)                                                                                                                                    cTCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCAGATAGATATCCAGTTCACCGGAAGTAATCGCCCcgc   >  1:841385/1‑140 (MQ=255)                                                                                                                                     tCCTAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCcgcg  <  1:1504396/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCG  >  NC_000913/2219009‑2219280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑