Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,801,748	C→A	A134E (GCG→GAG)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,801,748	0	C	A	100.0%	149.7 / NA	43	A134E (GCG→GAG)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/19);  total (24/19)

TTTACCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTT  >  NC_000913/2801612‑2801884                                                                                                                                         |                                                                                                                                         tttACCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCt                                                                                                                                       >  1:460813/1‑140 (MQ=255)     cccACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACg                                                                                                                                   <  1:56680/140‑1 (MQ=255)           cacacaCCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGca                                                                                                                             >  2:156129/1‑140 (MQ=255)           cacacaCCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGca                                                                                                                             >  2:430388/1‑140 (MQ=255)           cacacaCCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGca                                                                                                                             >  1:397104/1‑140 (MQ=255)            acacacCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGcac                                                                                                                            <  2:182708/140‑1 (MQ=255)            acacacCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGcac                                                                                                                            <  1:328087/140‑1 (MQ=255)             cacacCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGcaca                                                                                                                           <  1:63917/140‑1 (MQ=255)             cacacCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGcaca                                                                                                                           <  1:404274/140‑1 (MQ=255)                         tttCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGAc                                                                                                               <  2:460813/140‑1 (MQ=255)                            cGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCt                                                                                                            <  2:62377/140‑1 (MQ=255)                                tCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCg                                                                                                        <  1:430388/140‑1 (MQ=255)                                         tCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTg                                                                                               >  2:664521/1‑140 (MQ=255)                                           cTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGAc                                                                                             <  2:47814/140‑1 (MQ=255)                                           cTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGAc                                                                                             <  1:237126/140‑1 (MQ=255)                                           cTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGAc                                                                                             <  2:664891/140‑1 (MQ=255)                                               gggCATGGAAGTTTTACACCAGCTATACGTCGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGTTGAGACGCTGGCGTTGCAACTGACCGAt                                                                                         <  2:605771/140‑1 (MQ=255)                                                   aTGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGaaa                                                                                     >  1:740723/1‑140 (MQ=255)                                                   aTGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGaaa                                                                                     >  1:212473/1‑140 (MQ=255)                                                    tGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGaa                                                                                      >  1:596938/1‑138 (MQ=255)                                                    tGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGaa                                                                                      <  2:596938/138‑1 (MQ=255)                                                              acacCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGa                                                                          <  2:700772/140‑1 (MQ=255)                                                              acacCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGa                                                                          <  1:285821/140‑1 (MQ=255)                                                                    gCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCttt                                                                    >  1:302043/1‑140 (MQ=255)                                                                            ttGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTcagccagc                                                            <  1:651877/140‑1 (MQ=255)                                                                            ttGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTcagccagc                                                            >  1:713339/1‑140 (MQ=255)                                                                             tGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCagccagcc                                                           >  1:282076/1‑140 (MQ=255)                                                                              gAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAgccagcca                                                          >  1:694990/1‑140 (MQ=255)                                                                              gAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAgccagcca                                                          >  1:758578/1‑140 (MQ=255)                                                                               aaGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCAc                                                         >  1:636792/1‑140 (MQ=255)                                                                               aaGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCAc                                                         >  1:368556/1‑140 (MQ=255)                                                                                    aTTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCaa                                                    >  1:221553/1‑140 (MQ=255)                                                                                                       cTGGAAGATTTTGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTaa                                 >  1:245436/1‑140 (MQ=255)                                                                                                                 ttGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAgcgc                       >  2:189741/1‑140 (MQ=255)                                                                                                                  tGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGcgcg                      >  2:573661/1‑140 (MQ=255)                                                                                                                  tGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGcgcg                      <  2:694990/140‑1 (MQ=255)                                                                                                                  tGCCGATCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGcgcg                      <  1:307489/140‑1 (MQ=255)                                                                                                                        tCGAGTAACGATGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAAc                >  2:616515/1‑140 (MQ=255)                                                                                                                                  aTGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGt            >  2:444545/1‑134 (MQ=255)                                                                                                                                  aTGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGt            <  1:444545/134‑1 (MQ=255)                                                                                                                                  aTGGTGGAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTg      >  1:212204/1‑140 (MQ=255)                                                                                                                                    ggtggAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGtt    >  2:669321/1‑140 (MQ=255)                                                                                                                                      tggAGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGtttt  >  2:672108/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TTTACCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTT  >  NC_000913/2801612‑2801884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑