Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,743,850	C→A	G36G (GGC→GGA)	yiaL →	DUF386 domain‑containing protein YiaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,850	0	C	A	100.0%	155.3 / NA	45	G36G (GGC→GGA)	yiaL	DUF386 domain‑containing protein YiaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/22);  total (23/22)

TGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCG  >  NC_000913/3743729‑3743986                                                                                                                          |                                                                                                                                         tGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGc                                                                                                                        >  1:755694/1‑140 (MQ=255)     gagaTAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCaaaa                                                                                                                    >  1:443932/1‑140 (MQ=255)        aTAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAata                                                                                                                 >  2:463538/1‑140 (MQ=255)         tAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAatat                                                                                                                >  1:668124/1‑140 (MQ=255)                tGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACg                                                                                                         >  1:405702/1‑140 (MQ=255)                  aTTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCa                                                                                                       >  1:744458/1‑140 (MQ=255)                      ttGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAAtt                                                                                                   <  2:443932/140‑1 (MQ=255)                        ggACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTAt                                                                                                 <  2:17571/140‑1 (MQ=255)                              atCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGAttt                                                                                           >  1:706116/1‑140 (MQ=255)                                 gcgcAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                        <  2:405702/140‑1 (MQ=255)                                 gcgcAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                        <  2:365483/140‑1 (MQ=255)                                    cAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACcac                                                                                     <  2:145205/140‑1 (MQ=255)                                       cccAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACAcg                                                                                  >  2:275590/1‑140 (MQ=255)                                       cccAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACAcg                                                                                  >  2:49067/1‑140 (MQ=255)                                        ccAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACAcgc                                                                                 >  2:53744/1‑140 (MQ=255)                                            tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                             <  2:386252/140‑1 (MQ=255)                                            tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACAGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                             <  1:697117/140‑1 (MQ=255)                                            cccGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                             <  2:185179/139‑1 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                             <  1:63359/135‑1 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                             >  2:63359/1‑135 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                        >  2:36065/1‑140 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                        >  2:405876/1‑140 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                        >  1:568594/1‑140 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCg                                                                           >  2:604035/1‑137 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCg                                                                           <  1:604035/137‑1 (MQ=255)                                                 gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCG‑GTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtgg                                                                       >  1:547332/1‑140 (MQ=255)                                                    gTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTa                                                                     <  2:668124/140‑1 (MQ=255)                                                      ttGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTaaa                                                                   <  1:511228/140‑1 (MQ=255)                                                              cgccATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGACGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCgg                                                           <  2:604936/140‑1 (MQ=255)                                                                      aaaaGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAt                                                   <  2:11066/140‑1 (MQ=255)                                                                         aGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgt                                                <  2:223520/140‑1 (MQ=255)                                                                         aGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgt                                                <  2:462624/140‑1 (MQ=255)                                                                         aGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgt                                                <  2:200360/140‑1 (MQ=255)                                                                           gcgcTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgtcg                                              >  2:272890/1‑140 (MQ=255)                                                                              cTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGta                                           <  1:280900/140‑1 (MQ=255)                                                                              cTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGta                                           >  2:685956/1‑140 (MQ=255)                                                                                 gATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTatat                                        <  1:66750/140‑1 (MQ=255)                                                                                         gcgcgcTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTc                                <  1:658897/140‑1 (MQ=255)                                                                                           gcgcTACCGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAg                              >  2:421431/1‑140 (MQ=255)                                                                                                 ccGATTTCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTg                        <  1:49067/140‑1 (MQ=255)                                                                                                     tttCAACGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGt                    <  2:547332/140‑1 (MQ=255)                                                                                                           cGCCCTGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCg              >  2:241541/1‑140 (MQ=255)                                                                                                                tGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAagag         >  2:590601/1‑140 (MQ=255)                                                                                                                tGGAGCCGGGAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAagag         >  2:602526/1‑140 (MQ=255)                                                                                                                       gggAGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCg  >  1:586006/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                         TGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCG  >  NC_000913/3743729‑3743986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑