Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,072	C→A	A213S (GCC→TCC)	fsaB ←	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,072	0	C	A	100.0%	237.8 / NA	67	A213S (GCC→TCC)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/32);  total (35/32)

CTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTT  >  NC_000913/4138936‑4139206                                                                                                                                         |                                                                                                                                       cTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAAtt                                                                                                                                     <  1:476166/140‑1 (MQ=255)   aaCCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTcc                                                                                                                                   >  2:215153/1‑140 (MQ=255)    aCCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCa                                                                                                                                  >  1:275650/1‑140 (MQ=255)        gCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCg                                                                                                                              >  2:361431/1‑140 (MQ=255)         cGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCGCATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCgt                                                                                                                             <  1:285912/140‑1 (MQ=255)         cGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCgt                                                                                                                             >  1:19728/1‑140 (MQ=255)         cGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCgt                                                                                                                             >  1:2678/1‑140 (MQ=255)              tGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCg                                                                                                                        <  1:20652/140‑1 (MQ=255)              tGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCg                                                                                                                        >  1:240032/1‑140 (MQ=255)                          cAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCtata                                                                                                            >  1:51947/1‑140 (MQ=255)                               aTTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGa                                                                                                       <  1:290027/140‑1 (MQ=255)                                 tCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGAct                                                                                                     >  2:219925/1‑140 (MQ=255)                                  cGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGActc                                                                                                    <  1:640455/140‑1 (MQ=255)                                    cccAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTa                                                                                                  <  2:2678/140‑1 (MQ=255)                                     ccAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTAc                                                                                                 <  1:371141/140‑1 (MQ=255)                                        gACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGc                                                                                              >  1:257760/1‑140 (MQ=255)                                         aCGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCa                                                                                             <  1:647894/140‑1 (MQ=255)                                            aTTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAggg                                                                                          <  1:608265/140‑1 (MQ=255)                                            aTTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAggg                                                                                          <  2:387268/140‑1 (MQ=255)                                               aaTCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGgtg                                                                                       <  2:726713/140‑1 (MQ=255)                                                 tCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGtt                                                                                     >  2:436695/1‑140 (MQ=255)                                                  cacaTCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTg                                                                                    <  2:38015/140‑1 (MQ=255)                                                     aTCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGc                                                                                 >  1:714177/1‑140 (MQ=255)                                                     aTCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGc                                                                                 >  2:462620/1‑140 (MQ=255)                                                           gcCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgt                                                                           <  2:629417/140‑1 (MQ=255)                                                             ccTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgttg                                                                         <  2:327721/140‑1 (MQ=255)                                                             ccTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgttg                                                                         <  1:187073/140‑1 (MQ=255)                                                              cTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTgc                                                                        >  1:488812/1‑140 (MQ=255)                                                               tGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgt                                                                           >  1:357911/1‑136 (MQ=255)                                                               tGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgt                                                                           <  2:357911/136‑1 (MQ=255)                                                               tGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTgcg                                                                       <  2:275650/140‑1 (MQ=255)                                                               tGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTgcg                                                                       <  1:219925/140‑1 (MQ=255)                                                               tGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTgcg                                                                       <  2:332285/140‑1 (MQ=255)                                                                  aTGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTa                                                                    <  2:180649/140‑1 (MQ=255)                                                                      aTTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATc                                                                <  1:666259/140‑1 (MQ=255)                                                                       tttACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCt                                                               >  2:311299/1‑140 (MQ=255)                                                                       tttACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCt                                                               <  2:181847/140‑1 (MQ=255)                                                                           cccGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAgg                                                           >  2:13012/1‑140 (MQ=255)                                                                               gTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAg                                                       >  2:132592/1‑140 (MQ=255)                                                                               gTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAg                                                       >  2:584288/1‑140 (MQ=255)                                                                               gTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAg                                                       >  1:380799/1‑140 (MQ=255)                                                                                 gATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGTGCAggg                                                     >  1:410791/1‑140 (MQ=255)                                                                                    tGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGa                                                  <  2:51947/140‑1 (MQ=255)                                                                                      aataatGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATg                                                <  1:584288/140‑1 (MQ=255)                                                                                          atGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGAtt                                            <  2:488812/140‑1 (MQ=255)                                                                                          atGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGAtt                                            >  2:238526/1‑140 (MQ=255)                                                                                                tCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATc                                      >  1:233064/1‑140 (MQ=255)                                                                                                 ccATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATcc                                     <  1:462620/140‑1 (MQ=255)                                                                                                 ccATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATcc                                     <  1:215153/140‑1 (MQ=255)                                                                                                 ccATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATcc                                     <  1:517878/140‑1 (MQ=255)                                                                                                 ccATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATcc                                     <  1:13012/140‑1 (MQ=255)                                                                                                  cATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCt                                    >  1:723803/1‑140 (MQ=255)                                                                                                          cTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTaa                            >  2:423525/1‑140 (MQ=255)                                                                                                          cTCCTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTaa                            >  1:6558/1‑140 (MQ=255)                                                                                                            ccTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAAc                          >  1:594591/1‑140 (MQ=255)                                                                                                            ccTTTAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAAc                          <  2:380799/140‑1 (MQ=255)                                                                                                               ttAGAGATGAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGt                       >  1:586438/1‑140 (MQ=255)                                                                                                                       gAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGTTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGcc               >  1:470382/1‑140 (MQ=255)                                                                                                                       gAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGcc               >  2:353507/1‑140 (MQ=255)                                                                                                                       gAGTAGTGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGcc               >  2:287466/1‑140 (MQ=255)                                                                                                                        agtagtGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCt              >  1:456632/1‑140 (MQ=255)                                                                                                                            gtGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGAcg          <  2:449251/140‑1 (MQ=255)                                                                                                                            gtGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGAcg          <  2:233064/140‑1 (MQ=255)                                                                                                                             tGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGAcgc         >  1:417973/1‑140 (MQ=255)                                                                                                                             tGCCAAATGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGAcgc         >  1:679462/1‑140 (MQ=255)                                                                                                                                    tGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGttt  >  2:365081/1‑140 (MQ=255)                                                                                                                                    tGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGttt  >  1:283785/1‑140 (MQ=255)                                                                                                                                    tGCGGAATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGttt  >  2:60443/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTT  >  NC_000913/4138936‑4139206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑