| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,444 | 0 | T | A | 38.5% | 25.6 / 11.4 | 26 | intergenic (‑188/‑19) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (0/16); new base A (10/0); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAA > NC_000913/3206315‑3206575 | tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCTTCCAaaaaaaaaaaacg > 1:293829/1‑137 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑g < 1:440568/140‑1 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAAAAAAAAATGGA‑GGTga > 1:132701/1‑139 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGA‑GGTgg > 1:687112/1‑139 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTCCACACAAATAAAAAAAATGGA‑GGTGc > 1:304011/1‑140 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGcc > 2:409401/1‑140 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAAAAAAAAAAAAGGA‑GTTGGCatta > 1:204697/1‑138 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAg < 1:248944/140‑1 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAg < 1:409401/140‑1 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAg < 1:243642/140‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAAAAAAAAAATGGG‑GTTGCC‑TGATGAggg > 2:248944/1‑138 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGa < 1:307960/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGa < 1:400853/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGa < 1:131010/140‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAACAAAAAAAAAAAAATGA‑GTTGCCCTGATTAGCGaaaaa > 1:370679/1‑138 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATTGA‑GGTGCCATGATGAGCGaaaaa > 2:400853/1‑138 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATTGA‑GGTGCCATGATGAGGGaaaaga > 2:307960/1‑137 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAAAAAAAAATGGA‑GTTGCCCTGATGAGCGAAAGtaa > 1:84603/1‑140 (MQ=255) tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaataa < 2:402735/140‑1 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa < 1:429055/140‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAAGAGGTTGCCA‑GAAGAGAGGAAGGAAAAAGCAACACACAAGGAATAAAATGAAAGAGAt > 2:471885/1‑140 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGA‑GTTGGCCTGATGAGGGGAAATAATAAGCAAAAAGCAGTGAATAAATTGACAgagaa > 2:326186/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAAATTTTTCAAACAAAAACAAAAAAATGA‑GGTGCCATTTTGAGGGAAAGAAAAAAAAAAAAAGCAGTGAATAAGTTGACAgagaa > 2:728556/1‑139 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGGCATGATGAGGGAAAAGAAAAAAAAAAAAGCAGTGAAAAAAATTACAgagaatt > 2:114771/1‑137 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGA‑GGTGGCATGATGAGGGAAAATAAAAAACAAAAAGCAGTGAAAAAAATGACAGAGAttt > 2:337016/1‑139 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg < 1:101902/140‑1 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAAAAAAAAATGGA‑GGTGGCATGATGAGGGAAAAGAAAAAGAAAAAGGCAGGGAAAAAATTGAAAGAGATTTTTGCTa > 2:218482/1‑140 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTAGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGA‑GGTGGCCAGATGAGCGAAAGGAATAAACAACAAGCAGGGAATAAATTGAAAGAGAATGTTGCTaaa > 2:709944/1‑139 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATAGA‑GTTGGCCTGATGGGGGAAAGGAAAAAGCAAAAAGCAGGGAATAAGGTTAAAGAGATTGTTGCTAACTTTACCGCCCTGAttttt > 2:268701/1‑138 (MQ=255) tAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa < 1:409884/140‑1 (MQ=255) gTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAAt < 2:84603/140‑1 (MQ=255) gTAGTTTGTGGCGGT‑AAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc < 1:479834/140‑1 (MQ=255) tttGTGGGGGT‑GATTTTTTCCAGAAAAATACAAAAACGGGA‑GTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAGGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat < 2:304011/140‑1 (MQ=255) tttGTGGCGGT‑GATTTTTTCAAAACAAATAAAAAAACTGGA‑GTTGCCATGATGAGCAAAAGAAATAACCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat < 2:687112/140‑1 (MQ=255) ggggCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc < 1:268701/138‑1 (MQ=255) ttttCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa < 1:337016/140‑1 (MQ=255) cAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa < 2:597395/140‑1 (MQ=255) aaGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa < 2:204697/139‑1 (MQ=255) | TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAA > NC_000913/3206315‑3206575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |