| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,568,504 | 0 | T | G | 35.2% | 92.2 / 13.1 | 55 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base T (8/27); new base G (19/0); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC > NC_000913/4568372‑4568643 | ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTaaaaaaa < 2:261741/140‑1 (MQ=255) cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAATAAACGTGGCATa < 2:713394/140‑1 (MQ=255) cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAAAGTGGCATa > 1:347348/1‑140 (MQ=255) ggCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGCGGCCTaaaaa > 2:220553/1‑140 (MQ=255) aaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTc < 2:407420/140‑1 (MQ=255) aaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTc < 1:664740/140‑1 (MQ=255) aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTcc < 1:47285/140‑1 (MQ=255) cACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTg < 1:311054/140‑1 (MQ=255) ccACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGga > 1:534351/1‑140 (MQ=255) tGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAATGGCATAAAAAACAACTCCTTGTTCGGAGAAccccca > 2:509206/1‑138 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAACAAAAAACTCCTTTTCCCGAGAACCCCAACg > 1:275053/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGGGGCCTAAAAAACAAAACCCTGTCCGGAGAAACCCCAcc > 2:330480/1‑139 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGGGGCGGCATGGCGGGGAAAAAAAAAGGGGGTAAAAAAAAAACCCCTTTCCCGGGGAACCCCaag > 1:576811/1‑139 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACggg > 1:96289/1‑140 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGAGGCATAAAAAAAAAACCCTTGTCCGGGGAACCCCCACggg > 2:341799/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAACAAACAACTCCTTTTCCGGAGAACCCCAACgggg > 1:391984/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAAAGGCATAACAAACAACTCCTTTTCCGGAGAACCCCCACgggg > 2:365170/1‑140 (MQ=255) aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAAAGGGGCCTAAAAAAAAAAACCTTTTCCGGAGGACCCCCACcgggga > 2:738458/1‑138 (MQ=255) aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGGAAAAAAGAAGGCATAAAAAAAAACTCCTTGTCCCGAGAACCCCAACGGGGaa > 1:59259/1‑140 (MQ=255) aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAACAGGGATTACAAACAAATCCTTCTCCGGAGAAACCCAACggggga > 2:175652/1‑138 (MQ=255) gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAAAAAACAACTCCTTTTCCGGAGAACCCCAACGGGGgaaa > 1:293552/1‑140 (MQ=255) gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAAAAAAAAAAACCTTGTCCGGAGAACCCCAACGGGGaaaa > 2:393230/1‑140 (MQ=255) gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGCGGCATAACAAAAAAAACCTTGTCCGGGGAACCCCCACCGGGaaaa > 2:17494/1‑140 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACg < 1:222539/140‑1 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACg < 2:534351/140‑1 (MQ=255) gagATCAGATCCGTCGACAGAGACGCAGCAAAGTCATACAAAATCAACGCCATCGGCAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACACCTCCTTGTCCGGAGAACCCCAACGGGGAAAACg < 2:362055/138‑1 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAAAGTGGGATAAAAAAAAAAACCTTTTTCGGAGAAACCCCACGGGGGAAAAGaa > 2:766278/1‑140 (MQ=255) aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACg > 2:481788/1‑137 (MQ=255) aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACg < 1:481788/137‑1 (MQ=255) aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGCGGCATAAAAAAAAAATCCCTGTCCGGAGAACCCCCACGGGGGAAAAGaaa > 2:37467/1‑140 (MQ=255) tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAAt < 1:365170/140‑1 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAACAAACAACTCCTTTTCCGGAGAACCCCAACGGGGGAAAAGAAAATCAATaacc > 2:552951/1‑137 (MQ=255) aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa < 2:561167/140‑1 (MQ=255) aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAACAAAAAACTCCTTGTCCGGAGAACCCCCACGGGGAAAACGAAAATCAAAACCGTCGGTaa > 2:589769/1‑140 (MQ=255) caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa < 2:452395/140‑1 (MQ=255) aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg < 2:278952/140‑1 (MQ=255) aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGTGGCATAAAAAAAAAATCCTTGTCCGGAGAACCCCCACGGGGGAAAAGAAAATCAATACCCTCGGTAATGACGaaaag > 2:457652/1‑138 (MQ=255) aGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAAAGGGGCATAACAAAAAAATCCTTGTCCGGAGAACCCCAACGGGGGAAAAGAAAATCAATAACGTCGGGAATGACGaaaagg > 2:401213/1‑137 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGt < 1:552951/140‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga < 2:754045/140‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga < 1:37467/140‑1 (MQ=255) tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGGAAAAAAACGCGGCAAAAAAAAAAACTCCTTTTCCGGGGAAGCCCCACAGGGAAAACGAAAATCAAAACCCTCGGGAATGACGAAAAGCAGGGGAGAAagg > 2:289965/1‑140 (MQ=255) cGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGGAAACGAAATTCAATACCGTCGGGAATGACGAAAGGCAGGTGAGAAGGGCAAc > 1:449729/1‑140 (MQ=255) cGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc < 1:738458/140‑1 (MQ=255) aaaaaCAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa < 2:576811/140‑1 (MQ=255) aaaCAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAAt < 1:766278/140‑1 (MQ=255) aGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTAc < 2:96289/140‑1 (MQ=255) gTGGCGGCATGGC‑GGGTAAGAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGa < 2:279708/140‑1 (MQ=255) gTGGCGGCATGGC‑GGGTAAGAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGa > 1:279708/1‑140 (MQ=255) ggcggcATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAAt < 1:289965/140‑1 (MQ=255) cggcATGGC‑GGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTGTCCGGAGAACCCCAACGGGGAAAAAGAAAATCAATACCGTCGGTAATGACGAAAAGCAGGGGAGAAGGGCAACGAGACTGCCAAAGTACTGAAttt > 2:493359/1‑140 (MQ=255) cggcATGGC‑GGGGAAAAAAAAGGGGCATAACAAACAACTCCCTTTCCGGAGAAACCCAACGGGGGAAACGAAAATCAAAACCCTCGGTAAAGACGAAAAGCAGGGGAGAAGGGCAACGAGACTGCAAATGTACTGAAttt > 2:244661/1‑140 (MQ=255) aTGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg < 1:509206/140‑1 (MQ=255) tGGC‑GGGT‑AAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa < 2:660096/140‑1 (MQ=255) ggC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa < 1:31044/140‑1 (MQ=255) c‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAt < 1:401213/140‑1 (MQ=255) ggTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCa < 2:216309/140‑1 (MQ=255) tAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAc < 2:116058/140‑1 (MQ=255) | CCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGC‑GGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC > NC_000913/4568372‑4568643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |