Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,488	G→T	A362A (GCG→GCT)	ftsW →	putative lipid II flippase FtsW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,488	0	G	T	100.0%	150.1 / NA	43	A362A (GCG→GCT)	ftsW	putative lipid II flippase FtsW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/23);  total (20/23)

TAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAG  >  NC_000913/99352‑99623                                                                                                                                         |                                                                                                                                        tAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCgctgcg                                                                                                                                      >  2:359140/1‑140 (MQ=255)       tATTCTTCATCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCgggg                                                                                                                                <  1:78537/140‑1 (MQ=255)        aTTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCggggg                                                                                                                               >  1:491999/1‑139 (MQ=255)         ttcttcGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCgggggt                                                                                                                              >  2:243207/1‑138 (MQ=255)              cgtcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTAc                                                                                                                         >  1:221997/1‑140 (MQ=255)              cgtcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTAc                                                                                                                         >  2:58584/1‑140 (MQ=255)               gtcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTAcc                                                                                                                        >  1:153359/1‑140 (MQ=255)                tcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCg                                                                                                                       >  1:181535/1‑140 (MQ=255)                 cgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTTCCgc                                                                                                                      >  2:572867/1‑139 (MQ=255)                 cgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGa                                                                                                                      >  1:184041/1‑140 (MQ=255)                     tttCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCaa                                                                                                                  <  1:156494/140‑1 (MQ=255)                                     ttGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCg                                                                                                  <  1:133615/140‑1 (MQ=255)                                        gCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCCAAGGACTGACATTTCCGCTg                                                                                               >  2:126965/1‑140 (MQ=255)                                        gCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTg                                                                                               <  2:721706/140‑1 (MQ=255)                                           gTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATc                                                                                            <  1:58584/140‑1 (MQ=255)                                                  aTTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGTTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACg                                                                                     >  1:358141/1‑140 (MQ=255)                                                   ttAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACgg                                                                                    <  2:373319/140‑1 (MQ=255)                                                        aaTTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGtt                                                                               <  1:752772/140‑1 (MQ=255)                                                          ttGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCg                                                                             <  1:754476/140‑1 (MQ=255)                                                           tGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGGGGTTCgg                                                                            >  1:592456/1‑139 (MQ=255)                                                           tGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCAAAGGGCTGCCATTTCCGCTGATCAGTTACGGGGGtttgg                                                                            >  2:358071/1‑137 (MQ=255)                                                           tGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCCACCAAAGGGCTGACATTGCCGCTGATCAGTTACCGGGGTTCgg                                                                            >  2:106738/1‑139 (MQ=255)                                                            gACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGGGGGTCggg                                                                           >  1:483546/1‑138 (MQ=255)                                                             accaccGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGGTACCGACCAAAGGGCTGACATTTCCGCTGATCAGTTACGGGGGTTCGAGc                                                                          >  2:634110/1‑140 (MQ=255)                                                              ccaccGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCt                                                                         <  1:634110/140‑1 (MQ=255)                                                               caccGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCtt                                                                        <  2:107371/140‑1 (MQ=255)                                                                 ccGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTAc                                                                      <  2:583169/140‑1 (MQ=255)                                                                        tCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCCACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGGGGGTCGAGCTTACTGATTAt                                                               >  1:590295/1‑140 (MQ=255)                                                                             ttttCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGa                                                          <  1:650943/140‑1 (MQ=255)                                                                              tttCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGAc                                                         <  2:587971/140‑1 (MQ=255)                                                                                 ctcGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGc                                                      <  1:508804/140‑1 (MQ=255)                                                                                  tcGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGcc                                                     <  1:126965/140‑1 (MQ=255)                                                                                      cTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCcatca                                                 <  2:410785/140‑1 (MQ=255)                                                                                         ttCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCAAAAGGCTGACATTGCCGCTGATCAGTTACGGGGGGTCGAGCTTTCTGATTTTGTCGGCAGCCATCatga                                              >  2:283775/1‑140 (MQ=255)                                                                                           cTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCatgatg                                            <  2:153359/140‑1 (MQ=255)                                                                                               tGGCATCTGGTTTAGTTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGGCATCATGATGCTGt                                        <  2:358141/140‑1 (MQ=255)                                                                                                 gCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTg                                      <  1:429351/140‑1 (MQ=255)                                                                                                  cATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGc                                     <  2:181535/140‑1 (MQ=255)                                                                                                   aTCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTAACGACCAAAAGTCTGACATTTCCGCTGCTCAGCTACGGGGGGTTGAGGGTACTGATTATGTCGACAGCCATCATGATGCTGTTTCg                                    >  2:222946/1‑140 (MQ=255)                                                                                                      tGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTAt                                 <  1:359140/140‑1 (MQ=255)                                                                                                           tAGCTTCCAGGCGCTGGTTAACGTAGGCGCTGCGGCGGGGGTGTTACCGACCAAAGGGCTGACATTGCCGCTGATCAGTTACGGGGGTTCGAGCTTTCTGATTATGTCGACAGCCCTCATGATGCTGTTGCGGATTTAtt                            >  2:621739/1‑140 (MQ=255)                                                                                                                             tAACGTAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTATTGATTATGAAACGCGTCTGgaga          <  1:572867/140‑1 (MQ=255)                                                                                                                                  tAGGCGCTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAgcg     <  2:705799/140‑1 (MQ=255)                                                                                                                                     gcgcTGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGGGGGTCGAGCTTACTGGTTATGTCGGCAGCCATCATGATGCTGTTGCGGATTGATTATTAAACGCGGCTGGGGAAAACGCAg  >  2:489354/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGTGGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAG  >  NC_000913/99352‑99623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑