Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,195	G→C	G132A (GGC→GCC)	ygjJ →	protein YgjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,195	0	G	C	100.0%	83.7 / NA	24	G132A (GGC→GCC)	ygjJ	protein YgjJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (1/23);  total (1/23)

TGGTTCGGCCTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGGCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTGCGCTGAAGGTACAGAA  >  NC_000913/3228059‑3228327                                                                                                                                         |                                                                                                                                     tGGTTCGGCCTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCAc                                                                                                                                   <  1:320974/140‑1 (MQ=255)   gTTCGGCCTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCg                                                                                                                                 <  1:171829/140‑1 (MQ=255)   gTTCGGCCTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCg                                                                                                                                 <  1:245607/140‑1 (MQ=255)         ccTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATTGTACCAAAGATGCCAccccaaaca                                                                                                                           >  2:121988/1‑133 (MQ=255)                 aCGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGt                                                                                                                   <  1:697142/140‑1 (MQ=255)                              gACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGAtt                                                                                                      <  2:625601/140‑1 (MQ=255)                                        gCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATTGTTCCCAAGATGGCCCCCAAAACATGCTCAGTCTGCGTCAGGGTTTCTCCCTggg                                                                                            >  1:493082/1‑139 (MQ=255)                                         ccGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGGTATTTTTCCAAAAAAGGCACCCAAAAAAAACTCAGTCTCCGGCCGGAATGCTccccgggt                                                                                           >  2:563211/1‑133 (MQ=255)                                             ccACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGAcc                                                                                       <  1:646863/140‑1 (MQ=255)                                                       aaTGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAAtggtg                                                                             <  1:644661/140‑1 (MQ=255)                                                          gCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTgggg                                                                          <  1:182062/140‑1 (MQ=255)                                                           ccTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGc                                                                         <  1:182792/140‑1 (MQ=255)                                                                gagacAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGAttt                                                                    <  1:275443/136‑1 (MQ=255)                                                                     aGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCAccc                                                               <  1:706277/140‑1 (MQ=255)                                                                       aCCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCAccccc                                                             <  1:679228/140‑1 (MQ=255)                                                                                 gggTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCt                                                   <  2:193492/140‑1 (MQ=255)                                                                                    tggtTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACg                                                <  1:630321/140‑1 (MQ=255)                                                                                     ggtTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGt                                               <  1:258017/140‑1 (MQ=255)                                                                                       tttCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCa                                             <  2:628899/140‑1 (MQ=255)                                                                                                     ttAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCcgc                               <  2:84417/140‑1 (MQ=255)                                                                                                     ttAACCGTTTCTATGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCcgc                               <  1:115176/140‑1 (MQ=255)                                                                                                                 aTGGCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACt                   <  1:735376/140‑1 (MQ=255)                                                                                                                   ggCGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTgc                 <  1:62047/140‑1 (MQ=255)                                                                                                                     cGGTTATCGTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTgcgc               <  1:121988/140‑1 (MQ=255)                                                                                                                             gTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTGCGCTGAAGGTa       <  2:3988/140‑1 (MQ=255)                                                                                                                             gTACCAATGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTGCGCTGAAGGTa       <  2:493082/140‑1 (MQ=255)                                                                                                                                  aaTGATGCCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTGCGCTGAAGGTACAGaa  >  2:341162/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGGTTCGGCCTGATTTACGGCGAAGGCTGGACCGTGCAGGCCGACCACGACGGCAATGCCTGGGTGCCAGACCATAGCTGGGGTGGTTTCGAGGGCGGAATTAACCGTTTCTATGGCGGTTATCGTACCAATGATGGCACCGAAATCATGCTCAGTCTGCGTCAGGATTCCTCGCTGGATGACCTGCAATGGTGGGGCGATTTCACCCCCGATCTGGGCTACGTCATTCCCAATACCCGCGACATTATGACTGCGCTGAAGGTACAGAA  >  NC_000913/3228059‑3228327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑