Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,160,958 G→T Y198* (TAC→TAA)  fhuE ← ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,160,9580GT100.0% 155.9 / NA 44Y198* (TAC→TAA) fhuEferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/23);  total (21/23)

TTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTG  >  NC_000913/1160827‑1161094
                                                                                                                                   |                                                                                                                                        
ttttCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTccgc                                                                                                                                  >  1:96860/1‑140 (MQ=255)
            tAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGcc                                                                                                                      >  2:746603/1‑140 (MQ=255)
            tAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGcc                                                                                                                      >  2:155249/1‑140 (MQ=255)
                 gTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTaa                                                                                                                 >  2:699310/1‑140 (MQ=255)
                          tGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGAc                                                                                                        >  1:88503/1‑140 (MQ=255)
                            aGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTg                                                                                                      >  1:262101/1‑140 (MQ=255)
                            aGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTg                                                                                                      >  2:654259/1‑140 (MQ=255)
                            aGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTg                                                                                                      >  2:630707/1‑140 (MQ=255)
                              tCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTAAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt                                                                                                    <  1:502864/140‑1 (MQ=255)
                                 ttATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgc                                                                                                 >  2:364551/1‑140 (MQ=255)
                                 ttATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgc                                                                                                 >  1:610999/1‑140 (MQ=255)
                                      cTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGtt                                                                                            <  2:611057/140‑1 (MQ=255)
                                          tAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTTCCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCg                                                                                        <  2:253440/140‑1 (MQ=255)
                                          tAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCg                                                                                        <  2:96860/140‑1 (MQ=255)
                                          tAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCg                                                                                        <  1:140210/140‑1 (MQ=255)
                                          tAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCg                                                                                        <  2:219693/140‑1 (MQ=255)
                                            gccgccGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGaa                                                                                      <  1:312147/140‑1 (MQ=255)
                                              cgccgACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAAcc                                                                                    >  1:230543/1‑140 (MQ=255)
                                                ccgACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCat                                                                                  >  1:493195/1‑140 (MQ=255)
                                                           cgcgcgGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCg                                                                       <  1:339116/140‑1 (MQ=255)
                                                             cgcgGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCa                                                                     <  1:155249/140‑1 (MQ=255)
                                                             cgcgGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCa                                                                     <  1:9401/140‑1 (MQ=255)
                                                               cgGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGa                                                                   <  2:230543/140‑1 (MQ=255)
                                                                 gATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATg                                                                 >  2:653846/1‑140 (MQ=255)
                                                                             ttCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTcc                                                     <  2:123795/140‑1 (MQ=255)
                                                                                      tGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGa                                            >  1:431474/1‑140 (MQ=255)
                                                                                      tGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGa                                            >  1:50365/1‑140 (MQ=255)
                                                                                       gggCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAg                                           <  2:431474/140‑1 (MQ=255)
                                                                                              gTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTc                                    <  1:27477/140‑1 (MQ=255)
                                                                                                 aaagcGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTcgcg                                 <  2:548141/136‑1 (MQ=255)
                                                                                                 aaTCCGCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTcgcg                                 <  1:699310/140‑1 (MQ=255)
                                                                                                      gCCACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACg                            <  1:31984/140‑1 (MQ=255)
                                                                                                       ccACATACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc                           >  2:713526/1‑140 (MQ=255)
                                                                                                          caTACCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCact                        <  2:620450/140‑1 (MQ=255)
                                                                                                             aCCGTTCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCactact                     >  2:617849/1‑140 (MQ=255)
                                                                                                                 ttCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTa                 <  1:617849/140‑1 (MQ=255)
                                                                                                                 ttCTTTGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTa                 <  1:654259/140‑1 (MQ=255)
                                                                                                                    tttGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTAcgc              >  1:147385/1‑140 (MQ=255)
                                                                                                                     ttGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTAcgcg             >  2:77582/1‑140 (MQ=255)
                                                                                                                      tGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTa                 >  2:406160/1‑135 (MQ=255)
                                                                                                                      tGTTCCAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTa                 <  1:406160/135‑1 (MQ=255)
                                                                                                                           cAGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCaaa       <  1:77582/140‑1 (MQ=255)
                                                                                                                            aGCTACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAag      >  2:131928/1‑140 (MQ=255)
                                                                                                                                aCCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTg  <  1:713526/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                        
TTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTG  >  NC_000913/1160827‑1161094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑