Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,329	A→G	intergenic (‑243/+288)	yneO ← / ← lsrK	AIDA‑I family autotransporter YneO/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,329	0	A	G	100.0%	125.6 / NA	35	intergenic (‑243/+288)	yneO/lsrK	AIDA‑I family autotransporter YneO/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

AGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAA  >  NC_000913/1598193‑1598453                                                                                                                                         |                                                                                                                             aGGTGGGAAGGTGTCAATTTGGCTAATTTGCTTATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGgaaa                                                                                                                           >  2:42160/1‑140 (MQ=255)   gTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAAtt                                                                                                                         >  2:128000/1‑140 (MQ=255)       gAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTatca                                                                                                                     <  2:225827/140‑1 (MQ=255)            tgtCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTatcatcatc                                                                                                                <  1:652349/140‑1 (MQ=255)            tgtCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTatcatcatc                                                                                                                <  1:3251/140‑1 (MQ=255)                 aTTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATAtt                                                                                                           <  1:77178/140‑1 (MQ=255)                           tttGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGaa                                                                                                 <  2:357857/140‑1 (MQ=255)                                tAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCa                                                                                            <  1:152787/140‑1 (MQ=255)                                            aTTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTaaa                                                                                >  1:723455/1‑140 (MQ=255)                                                   gATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGaa                                                                         <  1:128000/140‑1 (MQ=255)                                                    aTTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAg                                                                        <  1:443131/140‑1 (MQ=255)                                                              gCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAAc                                                              >  1:340709/1‑140 (MQ=255)                                                                   gTTCTTATTTTATTTAAATACGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCtt                                                         <  1:392656/140‑1 (MQ=255)                                                                         attttatttAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAGCCCCTTTTGCAt                                                   <  2:245651/140‑1 (MQ=255)                                                                              atttaAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCAtt                                              >  2:631939/1‑140 (MQ=255)                                                                              atttaAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCAtt                                              >  1:536559/1‑140 (MQ=255)                                                                              atttaAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCAtt                                              >  1:14635/1‑140 (MQ=255)                                                                                 taAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAgt                                           <  2:527645/140‑1 (MQ=255)                                                                                 taAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAgt                                           <  2:241637/140‑1 (MQ=255)                                                                                 taAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAgt                                           <  2:723455/140‑1 (MQ=255)                                                                                 taAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAgt                                           <  1:262222/140‑1 (MQ=255)                                                                                              tGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAata                              >  1:697111/1‑140 (MQ=255)                                                                                              tGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAata                              >  1:644265/1‑140 (MQ=255)                                                                                                aGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATAtt                            <  1:591380/140‑1 (MQ=255)                                                                                                aGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATAtt                            <  2:340709/140‑1 (MQ=255)                                                                                                  ttATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCg                          <  1:594422/140‑1 (MQ=255)                                                                                                  ttATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCg                          >  2:594422/1‑140 (MQ=255)                                                                                                    aTTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCt                        >  2:331139/1‑140 (MQ=255)                                                                                                    aTTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCt                        >  1:131119/1‑140 (MQ=255)                                                                                                      tgttgCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCtat                      <  2:697111/140‑1 (MQ=255)                                                                                                          gCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATaaaa                  >  2:275750/1‑140 (MQ=255)                                                                                                          gCTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATaaaa                  >  2:595536/1‑140 (MQ=255)                                                                                                          acTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACTCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATaaaa                  >  1:476522/2‑140 (MQ=255)                                                                                                           cTTAATAAGAAAACTGTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAAt                 >  1:326321/1‑140 (MQ=255)                                                                                                                          gTTTCTTTCAATAGGGAAATTATCATCATCTTATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTaa  >  1:762682/1‑140 (MQ=255)                                                                                                                          gTTTCTTTCAATAGGGAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTaa  >  1:585801/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             AGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAA  >  NC_000913/1598193‑1598453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑