Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA31.7% 72.3 / 13.4 41intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/5);  new base A (0/13);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 4.86e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACC  >  NC_000913/752991‑753262
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ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                                       >  2:594343/1‑140 (MQ=255)
ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                                       >  2:721115/1‑140 (MQ=255)
        gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                               >  2:436707/1‑140 (MQ=255)
        gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                               >  2:277450/1‑140 (MQ=255)
         aCTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGc                                                                                                                              >  1:680605/1‑140 (MQ=255)
               aTGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATg                                                                                                                        >  1:720411/1‑140 (MQ=255)
                    tttttaAATTGAAAGTATTGGTTTGCTAAAAATTGGAGCTTTTTTTTTTTTTTAAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAAcgg                                                                                                                   <  2:595526/139‑1 (MQ=255)
                         tAATTAAAAGTTTTGGTTTGCTGATAATTTGAGCTTTTCTTTTTTTTTTAAATATTTATATAGGTTTTTTAATGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAAAAAAAAAATCACCCCGCCATATGAACGGCGGGt                                                                                                              <  2:422032/140‑1 (MQ=255)
                             agAAAGTATTGGGTTGCTGATATTTTGAGCTGTTCTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTaaa                                                                                                          <  2:620290/139‑1 (MQ=255)
                               aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAat                                                                                                        >  1:694055/1‑140 (MQ=255)
                                          ttGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAcct                                                                                             >  1:620290/1‑138 (MQ=255)
                                             cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                          >  1:62223/1‑140 (MQ=255)
                                                  aaTTTGAGCTTTTTTTTTTTTTTTAAATATTTATATAGGTCTTTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCa                                                                                     <  2:532894/140‑1 (MQ=255)
                                                       gAGCTTTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                                <  1:11490/140‑1 (MQ=255)
                                                       gAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                                >  1:210021/1‑140 (MQ=255)
                                                                    ttttttAAATATCTATATAGGTTTGTTAATGGTTTTTATTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                   <  2:210021/139‑1 (MQ=255)
                                                                     tttttAAATATCTATATAGGTCTTTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATc                                                                  <  2:211832/140‑1 (MQ=255)
                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTaa                                                                             <  1:199762/127‑1 (MQ=255)
                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTaa                                                                             >  2:199762/1‑127 (MQ=255)
                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  2:530805/1‑140 (MQ=255)
                                                                                 tatataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTc                                                     >  2:232379/1‑140 (MQ=255)
                                                                                    ataGGTCTTTTAATGGATTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTcgcg                                                  <  1:350049/140‑1 (MQ=255)
                                                                                    ataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTcgcg                                                  >  1:184174/1‑140 (MQ=255)
                                                                                     tgggTTTGTTAATGGTTTTTTTTTTTACAATTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGt                                                 <  2:694055/138‑1 (MQ=255)
                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGtttt                                              >  2:314754/1‑140 (MQ=255)
                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGtttt                                              >  2:330155/1‑140 (MQ=255)
                                                                                          ttattAATGGTTTTTATTTTTAAATTTTTTTGTGTTGAGGCATAAAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTc                                            <  2:294538/137‑1 (MQ=255)
                                                                                           tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCa                                           >  1:497597/1‑140 (MQ=255)
                                                                                           tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCa                                           >  1:621110/1‑140 (MQ=255)
                                                                                           tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAACCTTGATATCGC‑TTTTAAAGTCGCGtttttta                                           >  2:333817/1‑138 (MQ=255)
                                                                                            tttAATGGATTTTTTTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCat                                          <  1:207270/139‑1 (MQ=255)
                                                                                            tttAATGGATTTTATTTTTACATTTTTTTGTGTTTAGGAATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCat                                          <  1:594343/139‑1 (MQ=255)
                                                                                            tttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCat                                          <  1:721115/139‑1 (MQ=255)
                                                                                            tttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCat                                          <  2:386236/139‑1 (MQ=255)
                                                                                            gTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCat                                          <  2:621110/140‑1 (MQ=255)
                                                                                             ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCata                                         >  2:357922/1‑140 (MQ=255)
                                                                                             ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCata                                         >  1:479381/1‑140 (MQ=255)
                                                                                               aaTGGTTTTTTTTTTTAAATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATc                                       <  2:672403/140‑1 (MQ=255)
                                                                                               aaTGGTTTTTATTTTTACATTTTTTTGTTTTTAGCAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATc                                       <  1:136082/140‑1 (MQ=255)
                                                                                                     ttttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACCTAGCAACCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGtat                                 >  2:551753/1‑140 (MQ=255)
                                                                                                       ttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACCACCTCACCAACCACCATTAATGCCTGATATCCCGTTTTAAAGT‑GCGCTTTTTATATCCTGtattc                               >  2:579423/1‑138 (MQ=255)
                                                                                                                        tttgtTTAGCAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc              <  1:387209/138‑1 (MQ=255)
                                                                                                                        tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc              <  1:530805/140‑1 (MQ=255)
                                                                                                                                    tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAcc  >  2:224915/1‑140 (MQ=255)
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ATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGC‑TTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACC  >  NC_000913/752991‑753262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑