Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,698,692	G→T	R163R (CGC→CGA)	malI ←	DNA‑binding transcriptional repressor MalI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,698,692	0	G	T	100.0%	77.3 / NA	23	R163R (CGC→CGA)	malI	DNA‑binding transcriptional repressor MalI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/15);  total (8/15)

TAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCT  >  NC_000913/1698557‑1698825                                                                                                                                        |                                                                                                                                      tagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGaa                                                                                                                                   <  2:358127/140‑1 (MQ=255) tagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGaa                                                                                                                                   <  1:679265/140‑1 (MQ=255)  agagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAAc                                                                                                                                  <  2:329273/140‑1 (MQ=255)   gagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAAcc                                                                                                                                 <  1:270902/140‑1 (MQ=255)   gagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAAcc                                                                                                                                 <  1:456917/140‑1 (MQ=255)     gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGt                                                                                                                               >  1:766968/1‑140 (MQ=255)     gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGt                                                                                                                               <  1:510783/140‑1 (MQ=255)           caATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAAc                                                                                                                         <  2:357301/140‑1 (MQ=255)                gCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAAcatcat                                                                                                                    >  2:473244/1‑140 (MQ=255)                                                aaCTTTGCCCTCCCAACCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCAcc                                                                                    >  1:158421/1‑140 (MQ=255)                                                    ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGa                                                                                <  2:23683/140‑1 (MQ=255)                                                            ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCtttt                                                                        >  2:372371/1‑140 (MQ=255)                                                                                 gCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAgg                                                   >  1:351401/1‑140 (MQ=255)                                                                                  cccATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGt                                                  >  2:261871/1‑140 (MQ=255)                                                                                         cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCt                                           <  1:372371/140‑1 (MQ=255)                                                                                                           aacaacTGTGCAGCCTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGc                         >  1:405264/1‑140 (MQ=255)                                                                                                                         cTGCATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCAt           <  2:351401/140‑1 (MQ=255)                                                                                                                            cATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGa        <  2:158421/140‑1 (MQ=255)                                                                                                                            cATGTTGTCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGa        <  2:391688/140‑1 (MQ=255)                                                                                                                                ttgtCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACAcc    <  2:766968/140‑1 (MQ=255)                                                                                                                                  gtCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCt  <  2:210174/140‑1 (MQ=255)                                                                                                                                  gtCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCt  <  1:473244/140‑1 (MQ=255)                                                                                                                                  gtCCGGTCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCt  >  1:163858/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCT  >  NC_000913/1698557‑1698825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑