Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,747,774	G→T	G215V (GGT→GTT)	ydhS →	FAD/NAD(P) binding domain‑containing protein YdhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,774	0	G	T	100.0%	137.6 / NA	39	G215V (GGT→GTT)	ydhS	FAD/NAD(P) binding domain‑containing protein YdhS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/16);  total (23/16)

AGCAACCCGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGGTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTACG  >  NC_000913/1747643‑1747905                                                                                                                                    |                                                                                                                                    agCAACCCGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCa                                                                                                                             >  1:328906/1‑140 (MQ=255)    aaCCCGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTg                                                                                                                          >  2:173736/1‑140 (MQ=255)      cccGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGaa                                                                                                                        >  2:410704/1‑140 (MQ=255)      cccGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGaa                                                                                                                        >  1:44399/1‑140 (MQ=255)       ccGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAg                                                                                                                       <  2:44399/140‑1 (MQ=255)                   tCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAaacaac                                                                                                           <  2:625146/140‑1 (MQ=255)                    cccAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAacaaca                                                                                                          <  2:44313/140‑1 (MQ=255)                        gCCCGTGGTCAGGCCTGATGGAGGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACAcgtc                                                                                                      >  2:440890/1‑140 (MQ=255)                                   ggCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACcg                                                                                           >  1:8835/1‑140 (MQ=255)                                    gCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACcgc                                                                                          >  2:177403/1‑140 (MQ=255)                                               gCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATaa                                                                                     >  1:58020/1‑134 (MQ=255)                                               gCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATaa                                                                                     <  2:58020/134‑1 (MQ=255)                                                        gATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCt                                                                      <  1:177403/140‑1 (MQ=255)                                                          tGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTaa                                                                    <  1:669695/140‑1 (MQ=255)                                                                  aCGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACg                                                            <  1:440890/140‑1 (MQ=255)                                                                  aCGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACg                                                            <  1:425061/140‑1 (MQ=255)                                                                             atGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTcgcg                                                 <  1:520300/140‑1 (MQ=255)                                                                             atGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTcgcg                                                 <  1:693623/140‑1 (MQ=255)                                                                             atGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTcgcg                                                 <  2:328906/140‑1 (MQ=255)                                                                                 gAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACg                                             >  2:471476/1‑140 (MQ=255)                                                                                  aaCATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACgg                                            >  1:687036/1‑140 (MQ=255)                                                                                  aaCATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACgg                                            >  2:595052/1‑140 (MQ=255)                                                                                  aaCATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACgg                                            >  2:558845/1‑140 (MQ=255)                                                                                       ccTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTAtt                                       >  1:688654/1‑140 (MQ=255)                                                                                       ccTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTAtt                                       >  1:469796/1‑140 (MQ=255)                                                                                               ggACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGa                               >  2:136785/1‑140 (MQ=255)                                                                                                 aCTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTTAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAg                             >  1:497622/1‑140 (MQ=255)                                                                                                 aCTGGATGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAg                             >  1:592366/1‑140 (MQ=255)                                                                                                      aTGCGGCAATGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGAt                        <  1:471476/140‑1 (MQ=255)                                                                                                             aaTGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTAtt                 >  1:531961/1‑140 (MQ=255)                                                                                                               tGGCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGc               >  2:395055/1‑140 (MQ=255)                                                                                                                ggCAGTGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGcc              <  2:687036/140‑1 (MQ=255)                                                                                                                     tGGCTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTAtt         <  1:734949/140‑1 (MQ=255)                                                                                                                        cTATTCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTccc      <  2:8835/140‑1 (MQ=255)                                                                                                                           ttCATCATGTTTCGTTCATTGAAGATGATAAACAACACGTCG‑TTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTACg  <  1:173736/140‑1 (MQ=255)                                                                                                                           ttCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTAc   >  2:379507/1‑140 (MQ=255)                                                                                                                           ttCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTAc   >  2:185174/1‑140 (MQ=255)                                                                                                                           ttCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTAc   >  2:686865/1‑140 (MQ=255)                                                                                                                           ttCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTAc   >  1:166878/1‑140 (MQ=255)                                                                                                                            tCAGCATGTTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTACg  <  1:395055/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    AGCAACCCGAACGTATTTTCCCAGCCCGTGGTCAGGCCTGATGGAAGCAAAGGTCGATGCGTGTAACGTGGGTATTATGGGAACATCCTTGAGCGGACTGGATGCGGCAATGGCAGTGGCTATTCAGCATGGTTCGTTCATTGAAGATGATAAACAACACGTCGTTTTTCACCGCGATAACGCAAGTGAAAAGCTAAATATCACGTTGTTGTCGCGCACGGGTATTTTACCCGAAGCCGATTTCTATTGCCCTATTCCCTACG  >  NC_000913/1747643‑1747905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑