Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,044	C→A	G278V (GGC→GTC)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,044	0	C	A	100.0%	143.1 / NA	41	G278V (GGC→GTC)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/21);  total (20/21)

ATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAA  >  NC_000913/2318908‑2319171                                                                                                                                         |                                                                                                                                aTTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGGGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACaa                                                                                                                              >  1:725461/1‑140 (MQ=255)   tCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAAc                                                                                                                            <  2:124224/140‑1 (MQ=255)                    aGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCAt                                                                                                           <  2:328829/140‑1 (MQ=255)                           aCATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGa                                                                                                    <  1:642444/140‑1 (MQ=255)                              tGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGa                                                                                                 >  1:541222/1‑140 (MQ=255)                               gCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGAt                                                                                                <  2:26041/140‑1 (MQ=255)                               gCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTCTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGAt                                                                                                <  1:39188/140‑1 (MQ=255)                                 gAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCg                                                                                              >  2:149571/1‑140 (MQ=255)                                   aTGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGcc                                                                                            <  1:633983/140‑1 (MQ=255)                                    tGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGccc                                                                                           >  2:511014/1‑140 (MQ=255)                                    tGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGccc                                                                                           >  2:101846/1‑140 (MQ=255)                                     gCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCt                                                                                          <  2:636227/140‑1 (MQ=255)                                      cGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCtt                                                                                         >  2:551382/1‑140 (MQ=255)                                      cGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCtt                                                                                         >  2:270535/1‑140 (MQ=255)                                       gTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTa                                                                                        >  2:592765/1‑140 (MQ=255)                                          ccAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAAtt                                                                                     <  2:645216/140‑1 (MQ=255)                                             gCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTa                                                                                  <  2:225110/140‑1 (MQ=255)                                                  ttATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTc                                                                             >  2:269656/1‑140 (MQ=255)                                                   tATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCt                                                                            >  1:730145/1‑140 (MQ=255)                                                   tATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCt                                                                            >  2:128941/1‑140 (MQ=255)                                                      cAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGt                                                                         <  2:457245/140‑1 (MQ=255)                                                           ggCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGt                                                                    <  1:149571/140‑1 (MQ=255)                                                           ggCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGt                                                                    >  2:215132/1‑140 (MQ=255)                                                              aCCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCgg                                                                     <  2:168860/136‑1 (MQ=255)                                                              aCCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCgg                                                                     >  1:168860/1‑136 (MQ=255)                                                              aCCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAg                                                                 <  2:56660/140‑1 (MQ=255)                                                                    tACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAAt                                                           <  1:551382/140‑1 (MQ=255)                                                                               tAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGAc                                                <  2:128343/140‑1 (MQ=255)                                                                                     tGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCAtttt                                          >  1:4646/1‑140 (MQ=255)                                                                                       aGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTtca                                        <  2:4646/140‑1 (MQ=255)                                                                                          gTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTtcatca                                     <  1:244617/140‑1 (MQ=255)                                                                                            ggCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAg                                   <  1:514843/140‑1 (MQ=255)                                                                                             acAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGa                                  >  1:160472/2‑140 (MQ=255)                                                                                              cAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGaa                                 <  1:501453/140‑1 (MQ=255)                                                                                                                cAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTGTGACCATTTTCATCAAGAATGGTAACCCCCATCGGCa               >  2:750977/1‑140 (MQ=255)                                                                                                                cAGCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCa               >  2:203081/1‑140 (MQ=255)                                                                                                                  gCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAAc             >  2:383549/1‑140 (MQ=255)                                                                                                                  gCTCCCGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAAc             >  1:551575/1‑140 (MQ=255)                                                                                                                       cGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTAcc        <  1:704328/140‑1 (MQ=255)                                                                                                                       cGGAACCCTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTAcc        <  1:270535/140‑1 (MQ=255)                                                                                                                             ccTTCCGTATAGACAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTaa  >  2:467659/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                ATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAA  >  NC_000913/2318908‑2319171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑