| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,831,760 | G→T | T177K (ACG→AAG) | norR ← | DNA‑binding transcriptional dual regulator NorR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,831,760 | 0 | G | T | 97.3% | 125.1 / ‑2.7 | 37 | T177K (ACG→AAG) | norR | DNA‑binding transcriptional dual regulator NorR |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (18/18); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
TACCGGTTCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCGTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGCTGCATC > NC_000913/2831629‑2831894 | tACCGGTTCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCg < 1:65451/140‑1 (MQ=255) gTTCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTgg < 2:596199/130‑1 (MQ=255) gTTCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTgg > 1:596199/1‑130 (MQ=255) ttCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGgc > 2:556378/1‑140 (MQ=255) ccAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGgcag < 1:654374/140‑1 (MQ=255) ccAGTCTAACCGCTAATCAGGACGTTGAGATCGGCCGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGgcag < 2:86708/140‑1 (MQ=255) gATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCttt > 2:418790/1‑140 (MQ=255) tCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTcc > 1:264080/1‑140 (MQ=255) aGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAg < 2:635873/140‑1 (MQ=255) cGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAgttgt > 2:209811/1‑140 (MQ=255) tGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCa > 2:483390/1‑140 (MQ=255) tGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCa > 1:195460/1‑140 (MQ=255) tGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCa > 1:746958/1‑140 (MQ=255) agaTCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAAt < 2:746958/140‑1 (MQ=255) tCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAg < 2:734434/140‑1 (MQ=255) tCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAg > 2:137663/1‑140 (MQ=255) ggACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCGTCTGCGTCTGTTTCACCGCTTCAATCGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCa < 2:588787/140‑1 (MQ=255) gACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCaa > 1:455294/1‑140 (MQ=255) aCGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAAc < 2:195460/140‑1 (MQ=255) atctcTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTcc > 2:742509/1‑140 (MQ=255) aGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAgcgc > 1:245231/1‑140 (MQ=255) tGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCGTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCgcagc > 1:721225/1‑140 (MQ=255) tCATGCCGGGGGCCAGGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATc < 2:39873/140‑1 (MQ=255) tCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATc < 1:49585/140‑1 (MQ=255) cAAGCCAATCATCTGCGTCTGTTTCCCCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCtt < 2:753243/140‑1 (MQ=255) cAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCtt > 2:596850/1‑140 (MQ=255) aGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTtcg > 1:427368/1‑140 (MQ=255) tcatcTGCGTCTGTTTCACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTg > 2:357564/1‑140 (MQ=255) cACCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGAt < 1:418790/140‑1 (MQ=255) aCCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATc < 2:455294/140‑1 (MQ=255) aCCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATc > 1:686258/1‑140 (MQ=255) aCCGCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATc < 1:596850/140‑1 (MQ=255) gCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCggg < 1:17108/140‑1 (MQ=255) gCTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCggg < 2:571648/140‑1 (MQ=255) cTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGc > 2:152618/1‑140 (MQ=255) cTTCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGc > 1:102155/1‑140 (MQ=255) tCAAACGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGCTg < 2:696942/140‑1 (MQ=255) aCGGCTTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCGGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGCTGCATc < 1:209811/140‑1 (MQ=255) | TACCGGTTCCAGTCTCACCGCTGATCAGGACGTTGAGATCGGACGCCGCCACAATCTCAATCTCTTTTTTCAGTTGCGTCATGCCAGGGGACAAGCCAATCATCTGCGTCTGTTTCACCGCTTCAAACGGCGTGGCATCGCCTGGCAGCATATTCTGGCTTTCCAGTTGTTCAATCAGCAACGCATTGCTTAACGCTCCCGCCGCCAGCGCAGCAATCAGCCGTAGCTCTTCGTCGCTGAAAACATCGAACTGATCGGGCTGCATC > NC_000913/2831629‑2831894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |