Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,114,910	G→T	S1401R (AGC→AGA)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,114,910	0	G	T	100.0%	104.6 / NA	30	S1401R (AGC→AGA)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/15);  total (15/15)

TCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTG  >  NC_000913/3114774‑3115045                                                                                                                                         |                                                                                                                                        tCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTg                                                                                                                                      >  2:126403/1‑140 (MQ=255)         tCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAActc                                                                                                                              >  1:378798/1‑140 (MQ=255)             cTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGc                                                                                                                          >  1:662680/1‑140 (MQ=255)               gCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCag                                                                                                                        <  1:88427/140‑1 (MQ=255)                       gTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGc                                                                                                                <  1:756937/140‑1 (MQ=255)                        ttGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGcc                                                                                                               <  2:246819/140‑1 (MQ=255)                          ggATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCAt                                                                                                             <  2:144829/140‑1 (MQ=255)                          ggATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGCGCCAt                                                                                                             >  2:258487/1‑140 (MQ=255)                            aTTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCt                                                                                                           >  1:190836/1‑140 (MQ=255)                                aGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCAt                                                                                                             <  1:307793/134‑1 (MQ=255)                                aGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCAt                                                                                                             >  2:307793/1‑134 (MQ=255)                                  cacaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATAc                                                                                                     <  2:532150/140‑1 (MQ=255)                                                 cgccAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCa                                                                                      >  2:199811/1‑140 (MQ=255)                                                      aaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGtt                                                                                 <  1:199811/140‑1 (MQ=255)                                                           gTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTtct                                                                            <  2:70141/140‑1 (MQ=255)                                                                 gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGccc                                                                      <  1:542204/140‑1 (MQ=255)                                                                     aCCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGccc                                                                      >  1:6662/1‑136 (MQ=255)                                                                     aCCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGccc                                                                      <  2:6662/136‑1 (MQ=255)                                                                     aCCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTc                                                                  >  2:451747/1‑140 (MQ=255)                                                                        tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCtt                                                               <  2:378798/140‑1 (MQ=255)                                                                          aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                                             >  2:101479/1‑140 (MQ=255)                                                                          aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                                             >  1:23522/1‑140 (MQ=255)                                                                           tCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAg                                                            <  2:725063/140‑1 (MQ=255)                                                                                      tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa                                                 >  2:386103/1‑140 (MQ=255)                                                                                             tGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcagcag                                          >  2:149935/1‑140 (MQ=255)                                                                                              gCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa                                         <  1:570910/140‑1 (MQ=255)                                                                                                         aaCAGGTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc                              >  1:635009/1‑140 (MQ=255)                                                                                                             ggTTCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccg                          >  1:50695/1‑140 (MQ=255)                                                                                                               ttCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgcc                        <  2:137458/140‑1 (MQ=255)                                                                                                               ttCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgcc                        >  1:237398/1‑140 (MQ=255)                                                                                                                tCCAGCCTTTCATCCCTTCACGCTCTCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgccg                       <  2:190836/140‑1 (MQ=255)                                                                                                                                     gctctctGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGttgttg  <  2:579651/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTG  >  NC_000913/3114774‑3115045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑