Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,155,165 G→T R18R (CGC→CGA)  yqhC ← DNA‑binding transcriptional activator YqhC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,1650GT100.0% 187.8 / NA 53R18R (CGC→CGA) yqhCDNA‑binding transcriptional activator YqhC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/27);  total (26/27)

CCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAA  >  NC_000913/3155029‑3155299
                                                                                                                                        |                                                                                                                                      
ccGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGt                                                                                                                                     <  2:483932/140‑1 (MQ=255)
ccGGTGTACGGGCGAAAGGCGTCTCGCCATACAACAAACGCACACCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGt                                                                                                                                     <  2:23343/140‑1 (MQ=255)
   gtgtACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAAt                                                                                                                                  <  2:630728/140‑1 (MQ=255)
    tgtACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAAtt                                                                                                                                 >  2:301415/1‑140 (MQ=255)
      tACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTc                                                                                                                               >  1:9713/1‑140 (MQ=255)
        cGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCtt                                                                                                                             <  1:113562/140‑1 (MQ=255)
        cGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCtt                                                                                                                             >  2:32919/1‑140 (MQ=255)
        cGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCtt                                                                                                                             <  2:185535/140‑1 (MQ=255)
        cGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCtt                                                                                                                             >  1:676706/1‑140 (MQ=255)
           gCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAgg                                                                                                                          >  2:640660/1‑140 (MQ=255)
           gCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAgg                                                                                                                          >  2:42971/1‑140 (MQ=255)
              aaaGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGaat                                                                                                                       >  2:404837/1‑140 (MQ=255)
                  gCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATg                                                                                                                   >  1:517360/1‑140 (MQ=255)
                                  caaACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATtgtg                                                                                                   <  2:454760/140‑1 (MQ=255)
                                       gCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCAGCAATTTGATTGTGCGCaa                                                                                              <  1:42971/140‑1 (MQ=255)
                                            tCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTg                                                                                         >  2:632926/1‑140 (MQ=255)
                                             cGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGt                                                                                        >  2:733084/1‑140 (MQ=255)
                                               ggCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAg                                                                                      >  2:760890/1‑140 (MQ=255)
                                                gCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGc                                                                                     >  2:764815/1‑140 (MQ=255)
                                                gCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGc                                                                                     <  2:590009/140‑1 (MQ=255)
                                                  aaCAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCAt                                                                                   <  1:762350/140‑1 (MQ=255)
                                                  aaCAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCAt                                                                                   <  2:650521/140‑1 (MQ=255)
                                                    cAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGTAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCAttt                                                                                 <  2:673802/140‑1 (MQ=255)
                                                    cAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCAttt                                                                                 <  2:757947/140‑1 (MQ=255)
                                                         cTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCa                                                                            <  2:169741/140‑1 (MQ=255)
                                                            aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCa                                                                         <  1:404837/140‑1 (MQ=255)
                                                            aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCa                                                                         <  1:663442/140‑1 (MQ=255)
                                                                     tttCTTTATTTTTCAGCTTATTAACCTTATCCGCCAGCAAGCGGCAAATCTCTTCACGCTTCATATCTCGTAATTTCTTAGGAATAATACGGGAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGgaga                                                                >  1:707189/1‑140 (MQ=255)
                                                                        cTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAAt                                                             >  1:417475/1‑140 (MQ=255)
                                                                          ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAg                                                           >  2:278971/1‑140 (MQ=255)
                                                                           tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                          >  2:520624/1‑140 (MQ=255)
                                                                              ttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCaa                                                       >  2:592233/1‑140 (MQ=255)
                                                                                     tttATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                >  1:152132/1‑140 (MQ=255)
                                                                                      ttattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                               <  2:676706/140‑1 (MQ=255)
                                                                                        attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCa                                             <  1:100829/140‑1 (MQ=255)
                                                                                            aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                         <  1:32919/140‑1 (MQ=255)
                                                                                             cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAt                                        <  1:716944/140‑1 (MQ=255)
                                                                                              tttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATg                                       <  2:40297/140‑1 (MQ=255)
                                                                                               ttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                      >  2:19432/1‑140 (MQ=255)
                                                                                               ttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                      >  1:90107/1‑140 (MQ=255)
                                                                                                tATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAg                                     <  2:90107/140‑1 (MQ=255)
                                                                                                 aTCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                    >  2:700552/1‑140 (MQ=255)
                                                                                                 aTCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                    >  1:551054/1‑140 (MQ=255)
                                                                                                    cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                 <  2:551054/140‑1 (MQ=255)
                                                                                                    cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                 <  1:640660/140‑1 (MQ=255)
                                                                                                    cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                 <  1:520624/140‑1 (MQ=255)
                                                                                                       cAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                              >  2:696669/1‑140 (MQ=255)
                                                                                                       cAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                              <  2:31966/140‑1 (MQ=255)
                                                                                                       cAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                              <  2:9713/140‑1 (MQ=255)
                                                                                                                         ttCACGTTTCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGAt            <  2:152132/140‑1 (MQ=255)
                                                                                                                                ttCATATCTCGTAATTTCTTAGGGATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATg     >  1:633204/1‑140 (MQ=255)
                                                                                                                                ttCATATCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATg     >  1:472475/1‑140 (MQ=255)
                                                                                                                                   atatCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa  >  2:634208/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAA  >  NC_000913/3155029‑3155299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑