Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,179,596	G→T	R26L (CGT→CTT)	cobB →	protein‑lysine deacetylase/desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,179,596	0	G	T	100.0%	142.5 / NA	41	R26L (CGT→CTT)	cobB	protein‑lysine deacetylase/desuccinylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/25);  total (16/25)

TGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCC  >  NC_000913/1179459‑1179715                                                                                                                                          |                                                                                                                          tGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTa                                                                                                                         >  2:53723/1‑140 (MQ=255)       aggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTAtttttt                                                                                                                   >  1:483213/1‑140 (MQ=255)        ggaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTAttttttt                                                                                                                  <  1:769602/140‑1 (MQ=255)         gaATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTc                                                                                                                 <  1:384495/140‑1 (MQ=255)           aTGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAACCGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑ag                                                                                                             <  2:179743/140‑1 (MQ=255)           aTGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑ag                                                                                                             <  2:747821/140‑1 (MQ=255)                    gCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAgt                                                                                                    >  1:150393/1‑140 (MQ=255)                             cTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGa                                                                                           >  1:641961/1‑140 (MQ=255)                                      aCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGa                                                                                  >  1:354705/1‑140 (MQ=255)                                      aCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGa                                                                                  >  2:660607/1‑140 (MQ=255)                                      aCCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGa                                                                                  <  2:606159/140‑1 (MQ=255)                                          aTTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTCAGAGAGATAAAGTGGTGCCGGAAGCAATGGaaa                                                                                >  1:330845/1‑140 (MQ=255)                                           ttAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAAc                                                                             >  2:41547/1‑140 (MQ=255)                                                 aacaGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAagag                                                                       <  1:53723/140‑1 (MQ=255)                                                 aacaGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAagag                                                                       <  2:309280/140‑1 (MQ=255)                                                   caGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTa                                                                     <  1:334408/140‑1 (MQ=255)                                                   caGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTa                                                                     <  2:337313/140‑1 (MQ=255)                                                    agagGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTCAGAGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGt                                                                      <  2:330845/140‑1 (MQ=255)                                                    agagGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTAc                                                                    <  2:150393/140‑1 (MQ=255)                                                    agagGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTAc                                                                    <  2:246079/140‑1 (MQ=255)                                                         ttGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTa                                                               <  1:41547/140‑1 (MQ=255)                                                          tGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTAc                                                              >  2:105835/1‑140 (MQ=255)                                                            cTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTg                                                            <  1:246552/140‑1 (MQ=255)                                                              aTGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGAc                                                          <  1:105835/140‑1 (MQ=255)                                                               tGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACa                                                         <  2:641961/140‑1 (MQ=255)                                                                  tGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAggg                                                      <  1:217186/140‑1 (MQ=255)                                                                    tCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGGGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGc                                                    >  1:129002/1‑140 (MQ=255)                                                                    tCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAggg                                                      <  2:89487/138‑1 (MQ=255)                                                                    tCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAggg                                                      >  1:89487/1‑138 (MQ=255)                                                                    tCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGc                                                    >  1:437352/1‑140 (MQ=255)                                                                              gTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCt                                          >  2:113410/1‑140 (MQ=255)                                                                                        aaGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAg                                <  2:502383/140‑1 (MQ=255)                                                                                        aaGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAg                                <  1:660607/140‑1 (MQ=255)                                                                                           tCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTa                             >  2:57724/1‑140 (MQ=255)                                                                                                           aaaCGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCgcgc             <  2:354705/140‑1 (MQ=255)                                                                                                             aCGCCGCCTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGGGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGcgccg           <  2:129002/140‑1 (MQ=255)                                                                                                                  gccTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAt      <  2:483213/140‑1 (MQ=255)                                                                                                                   ccTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAt      <  2:180645/139‑1 (MQ=255)                                                                                                                   ccTGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAt      >  1:180645/1‑139 (MQ=255)                                                                                                                     tGCGCGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGc   <  2:437352/140‑1 (MQ=255)                                                                                                                      gcgcGAGCGTTTGCGTCAGCTTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGcc  >  1:422472/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                          TGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATAAACGCCGCCTGCGCGAGCGTTTGCGTCAGCGTATTTTTTTC‑‑AGAGATAAAGTGGTGCCGGAAGCAATGGAAAAACCAAGAGTACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCC  >  NC_000913/1179459‑1179715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑