Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,462,256	C→A	F44L (TTC→TTA)	paaK →	phenylacetate‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,462,256	0	C	A	100.0%	148.9 / NA	42	F44L (TTC→TTA)	paaK	phenylacetate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/21);  total (21/21)

TACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCG  >  NC_000913/1462121‑1462390                                                                                                                                        |                                                                                                                                       tACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACg                                                                                                                                    >  1:285832/1‑140 (MQ=255)  aCAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACgc                                                                                                                                   <  2:440782/140‑1 (MQ=255)        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAgg                                                                                                                             >  1:300560/1‑140 (MQ=255)                tACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATc                                                                                                                     >  2:624358/1‑140 (MQ=255)                tACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATc                                                                                                                     <  1:325562/140‑1 (MQ=255)                tACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATc                                                                                                                     <  2:375435/140‑1 (MQ=255)                      gCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCtgatg                                                                                                               >  2:175705/1‑140 (MQ=255)                       cTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCtgatga                                                                                                              >  1:433526/1‑140 (MQ=255)                          gACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGAttt                                                                                                           >  2:195171/1‑140 (MQ=255)                                aTTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGa                                                                                                     <  2:572990/140‑1 (MQ=255)                                aTTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGa                                                                                                     <  2:234108/140‑1 (MQ=255)                                aTTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGa                                                                                                     >  2:222624/1‑140 (MQ=255)                                 ttGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGaa                                                                                                    >  2:85216/1‑140 (MQ=255)                                 ttGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGaa                                                                                                    >  1:748841/1‑140 (MQ=255)                                          cgTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGAc                                                                                           >  2:555466/1‑140 (MQ=255)                                                          aCAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCt                                                                           >  1:165563/1‑140 (MQ=255)                                                                gTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTacca                                                                     <  2:165563/140‑1 (MQ=255)                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                                    >  2:559936/1‑140 (MQ=255)                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                                    <  2:189812/140‑1 (MQ=255)                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                                    <  1:199176/140‑1 (MQ=255)                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                                    <  1:559936/140‑1 (MQ=255)                                                                            gCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                                         <  1:769729/140‑1 (MQ=255)                                                                            gCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                                         <  2:60110/140‑1 (MQ=255)                                                                            gCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                                         >  1:519474/1‑140 (MQ=255)                                                                                  gAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGcgcg                                                   <  1:79191/140‑1 (MQ=255)                                                                                          cGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                                           >  1:596051/1‑140 (MQ=255)                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                          >  2:201127/1‑140 (MQ=255)                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                          <  2:761718/140‑1 (MQ=255)                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                          <  2:596051/140‑1 (MQ=255)                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                                          <  2:748841/140‑1 (MQ=255)                                                                                             tCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATccc                                        >  2:688092/1‑140 (MQ=255)                                                                                                aaCACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                                     >  1:411941/1‑140 (MQ=255)                                                                                                       gTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCt                              >  2:535713/1‑140 (MQ=255)                                                                                                       gTATGAAAATGTGCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCt                              >  2:749156/1‑140 (MQ=255)                                                                                                                 gtgCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc                    <  1:236951/140‑1 (MQ=255)                                                                                                                 gtgCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc                    <  1:201127/140‑1 (MQ=255)                                                                                                                   gCCGATGTATCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCaa                  >  1:695408/1‑140 (MQ=255)                                                                                                                            tCGGCGCAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAg         <  1:85216/140‑1 (MQ=255)                                                                                                                                 gcAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtggtg    <  1:749156/140‑1 (MQ=255)                                                                                                                                 gcAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtggtg    <  2:519474/140‑1 (MQ=255)                                                                                                                                 gcAAATTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAgtggtg    >  2:290774/1‑140 (MQ=255)                                                                                                                                   aaaTTAGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTgcg  <  1:283265/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCG  >  NC_000913/1462121‑1462390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑