Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,536,278	G→T	L114M (CTG→ATG)	narV ←	nitrate reductase Z subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,536,278	0	G	T	100.0%	167.5 / NA	48	L114M (CTG→ATG)	narV	nitrate reductase Z subunit gamma
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/21);  total (27/21)

CTTCATCATTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCAGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGAA  >  NC_000913/1536141‑1536413                                                                                                                                          |                                                                                                                                        cTTCATCATTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATcc                                                                                                                                       >  1:136666/1‑140 (MQ=255)   tcatcaTTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTg                                                                                                                                     >  2:747924/1‑140 (MQ=255)   tcatcaTTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTg                                                                                                                                     >  2:520869/1‑140 (MQ=255)      tcatTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTgcgc                                                                                                                                  <  1:25713/140‑1 (MQ=255)                 ccATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTcc                                                                                                                       >  2:267234/1‑140 (MQ=255)                           aCTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCa                                                                                                             >  1:346989/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  2:652520/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  2:620385/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  2:368198/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  2:383082/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  1:545650/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  1:616432/1‑140 (MQ=255)                               tgCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTAGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCa                                                                                                         >  1:163612/1‑140 (MQ=255)                                  tGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACg                                                                                                      <  2:262873/140‑1 (MQ=255)                                   aaaaaCGGTATAGTACTAAGCCCCAACAGACACTGCATCAGCAAAATGCCAATGTTGTTGCTAACCGGCGTAGTGGAAGTGGCCCGGTCGCGCTGATTGTTCATCCTGCGCCACAGCAGCCCTGCGCGGCCAATCAAGGt                                                                                                     <  2:456780/139‑1 (MQ=255)                                         ggTATGGTACTTAGCCCCAACAGACACTAGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAacac                                                                                               <  2:163612/140‑1 (MQ=255)                                                          cAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGa                                                                              >  2:621620/1‑140 (MQ=255)                                                                acacTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCa                                                                        <  1:55847/140‑1 (MQ=255)                                                                 cacTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCAt                                                                       >  1:624907/1‑140 (MQ=255)                                                                     ggATCAGCAAAATGCTCATGATGATGATATCCGGTGTAGTGGAAGTGGCACGCACGCGCTGATTTATCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCCACACACCGCAAATACCGCCGAGCACCATCGcc                                                                   <  1:632871/140‑1 (MQ=255)                                                                          aGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAg                                                              >  1:557715/1‑140 (MQ=255)                                                                           gCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAgt                                                             >  2:297172/1‑140 (MQ=255)                                                                              aaaTGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAgttgt                                                          >  2:368961/1‑140 (MQ=255)                                                                                        gatgatgatATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCa                                                <  2:651463/140‑1 (MQ=255)                                                                                        gatgatgatATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGCTTCGCTGCCa                                                <  1:620385/140‑1 (MQ=255)                                                                                           gatgatATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTg                                             <  2:211051/140‑1 (MQ=255)                                                                                                    cGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAAcc                                    >  2:21071/1‑140 (MQ=255)                                                                                                     ggCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCa                                   <  2:557715/140‑1 (MQ=255)                                                                                                     ggCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCa                                   <  2:136666/140‑1 (MQ=255)                                                                                                     ggCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCa                                   <  2:126819/140‑1 (MQ=255)                                                                                                     ggCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCa                                   <  1:171070/140‑1 (MQ=255)                                                                                                     ggCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCa                                   <  1:520869/140‑1 (MQ=255)                                                                                                      gCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCAc                                  >  2:602728/1‑140 (MQ=255)                                                                                                      gCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCAc                                  >  2:640926/1‑140 (MQ=255)                                                                                                       cGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAAc                                     <  2:223942/136‑1 (MQ=255)                                                                                                       cGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAAc                                     >  1:223942/1‑136 (MQ=255)                                                                                                       cGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCAcg                                 <  1:267234/140‑1 (MQ=255)                                                                                                       cGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCAcg                                 >  2:94964/1‑140 (MQ=255)                                                                                                          aGTGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGt                              <  1:640926/140‑1 (MQ=255)                                                                                                            tGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTAc                            >  2:447201/1‑140 (MQ=255)                                                                                                            tGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTAc                            >  1:465747/1‑140 (MQ=255)                                                                                                            tGGAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTAc                            <  1:447201/140‑1 (MQ=255)                                                                                                              gAAGTGGCACGCACGCGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACAt                          <  1:383082/140‑1 (MQ=255)                                                                                                                         cacgcGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGt                              >  1:701355/1‑125 (MQ=255)                                                                                                                         cacgcGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGt                              <  2:701355/125‑1 (MQ=255)                                                                                                                         cacgcGCTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTgcggcg               >  2:670424/1‑140 (MQ=255)                                                                                                                              gcTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAAc          <  1:368961/140‑1 (MQ=255)                                                                                                                               cTGATTTGTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACa         <  1:621620/140‑1 (MQ=255)                                                                                                                                      gTCATCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGaa  >  1:85475/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CTTCATCATTTCGCTACCATCAGGATACTGTGCTGAAAACGGTATGGTACTTAGCCCCAACAGACACTGGATCAGCAAAATGCTCATGATGATGATATCCGGCGTAGTGGAAGTGGCACGCACGCGCTGATTTGTCAGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGAA  >  NC_000913/1536141‑1536413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑