Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	586,653	G→T	P86T (CCG→ACG)	envY ←	DNA‑binding transcriptional activator EnvY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	586,653	0	G	T	100.0%	248.1 / NA	71	P86T (CCG→ACG)	envY	DNA‑binding transcriptional activator EnvY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/44);  total (27/44)

CTCAAGAAAGTTAGACAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGGGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGT  >  NC_000913/586516‑586787                                                                                                                                            |                                                                                                                                       cTCAAGAAAGTTAGACAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCgtgg                                                                                                                                      >  2:566389/1‑140 (MQ=255)   cAAGAAAGTTAGACAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGtt                                                                                                                                    >  2:583899/1‑140 (MQ=255)     aGAAAGTTAGACAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGc                                                                                                                                  <  2:603666/140‑1 (MQ=255)               aCAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGAc                                                                                                                        <  1:111679/140‑1 (MQ=255)               aCAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGAc                                                                                                                        <  2:10283/140‑1 (MQ=255)               aCAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGAc                                                                                                                        <  1:273756/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  2:776149/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  1:816974/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  1:583899/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  1:500522/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  2:631336/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  2:828539/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  2:511494/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  1:426170/140‑1 (MQ=255)                 aaTACGGTAAAAAGCAATGCGCGCGTCAACTCCACTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCa                                                                                                                      <  1:769861/140‑1 (MQ=255)                   tACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAgg                                                                                                                    >  2:749540/1‑140 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCGGTGTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAg                                                                                                                     >  2:245413/1‑140 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGAc                                                                                                                        <  1:821199/135‑1 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGAc                                                                                                                        >  2:821199/1‑135 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAggg                                                                                                                   <  1:477023/140‑1 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAggg                                                                                                                   <  1:310330/140‑1 (MQ=255)                    aCGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAggg                                                                                                                   <  2:67728/140‑1 (MQ=255)                     cGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGc                                                                                                                  >  2:922719/1‑140 (MQ=255)                           aaaGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTaca                                                                                                            <  1:621802/140‑1 (MQ=255)                            aatccaTGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACGCTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTacac                                                                                                           <  2:889181/135‑1 (MQ=255)                             aGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAggg                                                                                                                   <  2:125927/131‑1 (MQ=255)                             aGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAggg                                                                                                                   >  1:125927/1‑131 (MQ=255)                             aGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTAcaca                                                                                                          >  1:588047/1‑140 (MQ=255)                             aGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTAcaca                                                                                                          >  1:884938/1‑140 (MQ=255)                             aGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTAcaca                                                                                                          >  2:385281/1‑140 (MQ=255)                                                   cTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGGGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAaac                                                                                    >  2:866828/1‑140 (MQ=255)                                                   cTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTAttt                                                                                          >  1:272127/1‑134 (MQ=255)                                                   cTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTAttt                                                                                          <  2:272127/134‑1 (MQ=255)                                                        cAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAg                                                                               >  1:636539/1‑140 (MQ=255)                                                         aGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGa                                                                              >  1:397060/1‑140 (MQ=255)                                                           ggTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATa                                                                            <  2:445941/140‑1 (MQ=255)                                                             tCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAAt                                                                          <  2:954822/140‑1 (MQ=255)                                                               tgctgGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCGGTGTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAAt                                                                          <  1:245413/140‑1 (MQ=255)                                                                gctgGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGt                                                                       >  1:729260/1‑140 (MQ=255)                                                                gctgGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGt                                                                       >  2:921908/1‑140 (MQ=255)                                                                gctgGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGt                                                                       <  1:566389/140‑1 (MQ=255)                                                                    ggTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTtgat                                                                   <  2:884938/140‑1 (MQ=255)                                                                    ggTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTtgat                                                                   <  2:6249/140‑1 (MQ=255)                                                                     gTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTtgatg                                                                  <  2:331685/140‑1 (MQ=255)                                                                         ctgctTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGt                                                              >  2:25320/1‑140 (MQ=255)                                                                           gcttgctGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTa                                                            >  1:236833/1‑140 (MQ=255)                                                                                   tgGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAt                                                    <  1:749540/140‑1 (MQ=255)                                                                                    ggTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAtg                                                   <  1:42392/140‑1 (MQ=255)                                                                                     gTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAtgt                                                  >  2:432278/1‑140 (MQ=255)                                                                                      ttGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAtgtg                                                 <  2:690564/140‑1 (MQ=255)                                                                                        ggcggcTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTggg                                               >  2:287649/1‑140 (MQ=255)                                                                                             cTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAtgt                                                  <  2:54374/132‑1 (MQ=255)                                                                                             cTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGAtgt                                                  >  1:54374/1‑132 (MQ=255)                                                                                               aGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTa                                         <  2:558470/139‑1 (MQ=255)                                                                                               aGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTa                                         >  1:558470/1‑139 (MQ=255)                                                                                                gCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAAt                                       >  2:381/1‑140 (MQ=255)                                                                                                 ccGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATa                                      <  1:381/140‑1 (MQ=255)                                                                                                  cGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATAc                                     <  1:287649/140‑1 (MQ=255)                                                                                                   ggAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACg                                    <  1:222967/140‑1 (MQ=255)                                                                                                       cacTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGAt                                <  2:636539/140‑1 (MQ=255)                                                                                                          ttCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTa                             >  1:28975/1‑140 (MQ=255)                                                                                                          ttCCG‑‑GTGTACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTa                             <  1:922719/140‑1 (MQ=255)                                                                                                                    tACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTa                             <  2:4489/132‑1 (MQ=255)                                                                                                                    tACGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCATTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTa                             >  1:4489/1‑132 (MQ=255)                                                                                                                      cGGCTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGagag                   >  2:828387/1‑140 (MQ=255)                                                                                                                         cTATGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTc                <  1:921908/140‑1 (MQ=255)                                                                                                                            tGACAAGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAAt             <  2:729260/140‑1 (MQ=255)                                                                                                                                aaGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACg         <  1:25320/140‑1 (MQ=255)                                                                                                                                 aGCGATAACCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGt        >  2:505553/1‑140 (MQ=255)                                                                                                                                       aaCCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGagag                   >  1:47428/1‑123 (MQ=255)                                                                                                                                       aaCCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGagag                   <  2:47428/123‑1 (MQ=255)                                                                                                                                       aaCCGTGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTAttgt  <  2:28975/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       CTCAAGAAAGTTAGACAATACGGTAAAAAGCAATGCGCGCGTCAACTCCGCTTCGCAGGGTCTGCTGGGTTGCTGCTTGCTGTGGTTGGCGGCTAGCCGGAACACTTCCG‑‑GTGTACGGCTATGACAAGCGATAACCGGGGTTGCCAGCCGCGACCAGGGCTTTACACAGGTTAAGTTCTTATTTAAAAACAACAGATAATCGTTGATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGT  >  NC_000913/586516‑586787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑