Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	685,142	G→T	S38S (TCC→TCA)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	685,142	0	G	T	100.0%	235.5 / NA	67	S38S (TCC→TCA)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/35);  total (32/35)

CATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGT  >  NC_000913/685013‑685277                                                                                                                                  |                                                                                                                                         cATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACg                                                                                                                                <  2:570482/140‑1 (MQ=255)   tCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACggg                                                                                                                              >  1:85706/1‑140 (MQ=255)    cccGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGc                                                                                                                             <  1:669138/140‑1 (MQ=255)      cGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCg                                                                                                                           <  2:486051/140‑1 (MQ=255)        aCGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCa                                                                                                                         >  1:691341/1‑140 (MQ=255)           cgGGAACGCAGCTTCGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAac                                                                                                                      >  1:239689/1‑140 (MQ=255)              gAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAaccac                                                                                                                   >  1:348035/1‑140 (MQ=255)                  gCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCg                                                                                                                           <  1:183027/128‑1 (MQ=255)                  gCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCg                                                                                                                           >  2:183027/1‑128 (MQ=255)                    aGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACcaccaccac                                                                                                             >  2:677423/1‑140 (MQ=255)                      cTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACtt                                                                                                           <  1:677423/140‑1 (MQ=255)                      cTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACtt                                                                                                           <  1:702892/140‑1 (MQ=255)                                tCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCtttttt                                                                                                 >  2:635538/1‑140 (MQ=255)                                tCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCtttttt                                                                                                 >  2:491593/1‑140 (MQ=255)                                tCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGAC‑GGCCGCAAACCACCACCACTTCGCCTTTTTTc                                                                                                >  2:550907/1‑140 (MQ=255)                                     tttCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑Ctt                                                                                           <  2:404748/140‑1 (MQ=255)                                            tCGTTAACCACGATTCCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGa                                                                                    >  1:924121/1‑140 (MQ=255)                                            tCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGAC‑GGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAg                                                                                   <  1:550907/140‑1 (MQ=255)                                                 aaCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCTGG‑ACCAAACCGGCCCCCAACCACCCCCCATTCGCCTTTTTTCACCTCCGGTTggccatc                                                                               >  1:116150/1‑134 (MQ=255)                                                 aaCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGt                                                                               <  2:239689/140‑1 (MQ=255)                                                  aCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTc                                                                              >  1:80731/1‑140 (MQ=255)                                                     aCGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGt                                                                           <  2:390289/140‑1 (MQ=255)                                                     aCGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGt                                                                           <  1:965875/140‑1 (MQ=255)                                                      cGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTc                                                                          >  2:341035/1‑140 (MQ=255)                                                      cGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTc                                                                          >  2:736890/1‑140 (MQ=255)                                                      cGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTc                                                                          >  1:83659/1‑140 (MQ=255)                                                       gATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCa                                                                         <  1:341035/140‑1 (MQ=255)                                                         tACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGt                                                                               <  2:756959/132‑1 (MQ=255)                                                         tACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGt                                                                               >  1:756959/1‑132 (MQ=255)                                                           ccATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCAc                                                                     <  2:691341/140‑1 (MQ=255)                                                            cATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGc                                                                                  <  2:14881/126‑1 (MQ=255)                                                            cATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGc                                                                                  >  1:14881/1‑126 (MQ=255)                                                              tCGACGGTGATTTCACCTTGCTGCACTGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTg                                                                  >  2:604608/1‑140 (MQ=255)                                                                 aCGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGaaa                                                               >  1:621094/1‑140 (MQ=255)                                                                  cGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAg                                                              <  2:85706/140‑1 (MQ=255)                                                                   ggTGATTTTCCCCTGCTTCCCCCGGTCGAGGGCCGTGACGGTTTTAAACCAGGTTGGTTTGGCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGt                                                             <  2:116150/140‑1 (MQ=255)                                                                   ggTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGt                                                             <  2:621094/140‑1 (MQ=255)                                                                   ggTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGt                                                             <  1:384671/140‑1 (MQ=255)                                                                      gATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGac                                                          >  1:750862/1‑140 (MQ=255)                                                                       aTTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGt                                                             <  1:9067/136‑1 (MQ=255)                                                                       aTTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGt                                                             >  2:9067/1‑136 (MQ=255)                                                                       aTTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGacc                                                         >  1:225830/1‑140 (MQ=255)                                                                        tttCACCTTGCTGCACTGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGacca                                                        <  1:604608/140‑1 (MQ=255)                                                                            aCCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGaccatacc                                                    >  2:642211/1‑140 (MQ=255)                                                                                 gctgcACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCAtttt                                               >  2:408217/1‑140 (MQ=255)                                                                                 gctgcACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCAtttt                                               >  2:250948/1‑140 (MQ=255)                                                                                 gctgcACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCAtttt                                               >  1:905744/1‑140 (MQ=255)                                                                                  ctgcACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTg                                              >  2:532748/1‑140 (MQ=255)                                                                                           ttCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACTGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACAtttt                                     <  2:827505/140‑1 (MQ=255)                                                                                           ttCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACTGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACAtttt                                     >  1:827505/1‑140 (MQ=255)                                                                                            tCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACAttttt                                    <  2:299894/140‑1 (MQ=255)                                                                                             cGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTc                                   <  2:348035/140‑1 (MQ=255)                                                                                             cGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTc                                   <  2:472166/140‑1 (MQ=255)                                                                                              gAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATCCCATTTTGAAACATTTTTCa                                  <  1:250948/140‑1 (MQ=255)                                                                                              gAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCa                                  <  1:408217/140‑1 (MQ=255)                                                                                                  ccGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGt                              <  1:354452/140‑1 (MQ=255)                                                                                                  ccGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGt                              <  2:924121/140‑1 (MQ=255)                                                                                                  ccGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGt                              <  1:491593/140‑1 (MQ=255)                                                                                                  ccGTTGACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGt                              <  1:736890/140‑1 (MQ=255)                                                                                                       gACGGTTTTAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCa                         <  1:727106/140‑1 (MQ=255)                                                                                                               tAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTg                 >  1:412764/1‑140 (MQ=255)                                                                                                               tAATCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTg                 <  1:635538/140‑1 (MQ=255)                                                                                                                  tCAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGTCCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTcc              <  2:331965/140‑1 (MQ=255)                                                                                                                   cAGCGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCct             <  1:532748/140‑1 (MQ=255)                                                                                                                      cGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCcttct          >  2:603561/1‑140 (MQ=255)                                                                                                                      cGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCcttct          >  1:439311/1‑140 (MQ=255)                                                                                                                      cGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCcttct          >  2:820074/1‑140 (MQ=255)                                                                                                                      cGTTGATTTGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCcttct          >  1:880130/1‑140 (MQ=255)                                                                                                                              tGCCTGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGt  <  2:439311/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         CATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCA‑CTTCGGTTGAGCAGTCGGTCAGCACCTGAAAGTGACCATACCATTTTGAAACATTTTTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGT  >  NC_000913/685013‑685277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑