Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	713,692	C→A	S45S (TCC→TCA)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	713,692	0	C	A	100.0%	175.6 / NA	50	S45S (TCC→TCA)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/27);  total (23/27)

CAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCC  >  NC_000913/713556‑713824                                                                                                                                         |                                                                                                                                     cAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGt                                                                                                                                   <  2:909095/140‑1 (MQ=255)                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTGTGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAgcgc                                                                                                                >  1:246347/1‑140 (MQ=255)                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAgcgc                                                                                                                >  1:933899/1‑140 (MQ=255)                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAgcgc                                                                                                                >  1:721677/1‑140 (MQ=255)                        ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCca                                                                                                            <  1:750235/140‑1 (MQ=255)                        ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCca                                                                                                            <  1:125499/140‑1 (MQ=255)                        ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCca                                                                                                            <  1:289207/140‑1 (MQ=255)                        ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCca                                                                                                            <  1:246906/140‑1 (MQ=255)                              ccTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCtt                                                                                                      >  1:287063/1‑140 (MQ=255)                                       caGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGcc                                                                                             >  2:673204/1‑140 (MQ=255)                                           gTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcac                                                                                         <  1:240958/140‑1 (MQ=255)                                            tgatttgattAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcaca                                                                                        >  1:244386/1‑140 (MQ=255)                                                 tgattAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTg                                                                                   >  1:718283/1‑140 (MQ=255)                                                 tgattAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTg                                                                                   >  1:651162/1‑140 (MQ=255)                                                 tgattAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCACCGCGCCACAGC‑TTAACGAGCCGCACATTCTgg                                                                                  >  2:905677/1‑140 (MQ=255)                                                             cccAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAg                                                                       >  2:351077/1‑140 (MQ=255)                                                             cccAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAg                                                                       >  1:705455/1‑140 (MQ=255)                                                             cccAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAg                                                                       >  1:569900/1‑140 (MQ=255)                                                               cAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGc                                                                     <  2:569900/140‑1 (MQ=255)                                                                 aCTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCaa                                                                   <  2:933899/140‑1 (MQ=255)                                                                 aCTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTAGCGATCGCTCAGGCaa                                                                   <  2:651162/140‑1 (MQ=255)                                                                                ttatGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTg                                                    <  1:648936/140‑1 (MQ=255)                                                                                ttatGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTg                                                    <  1:673204/140‑1 (MQ=255)                                                                                  atGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCg                                                  <  2:718283/140‑1 (MQ=255)                                                                                      aCTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGaaaa                                              >  2:394818/1‑140 (MQ=255)                                                                                        tGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAAc                                            >  2:937855/1‑140 (MQ=255)                                                                                        tGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAAc                                            >  1:911819/1‑140 (MQ=255)                                                                                          aaaCCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACgg                                          >  2:100147/1‑140 (MQ=255)                                                                                          aaaCCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACgg                                          <  2:287063/140‑1 (MQ=255)                                                                                          aaaCCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACgg                                          >  2:280304/1‑140 (MQ=255)                                                                                            aCCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCa                                        >  2:754173/1‑140 (MQ=255)                                                                                               aGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCa                                     <  1:351077/140‑1 (MQ=255)                                                                                               aGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGAGAAAAACGGCATCa                                     >  1:897931/1‑140 (MQ=255)                                                                                                  aGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACt                                   >  2:450468/1‑139 (MQ=255)                                                                                                  aGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACt                                   <  1:450468/139‑1 (MQ=255)                                                                                                   gCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTgg                                 <  1:796653/140‑1 (MQ=255)                                                                                                    cAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGc                                >  1:213399/1‑140 (MQ=255)                                                                                                    cAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGc                                <  1:407965/140‑1 (MQ=255)                                                                                                      gggAATGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCTCCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGAGAAAAACGGCATCACTGGccc                              <  2:718014/140‑1 (MQ=255)                                                                                                          aTGCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCGCATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGc                          >  1:569569/1‑140 (MQ=255)                                                                                                            gCGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCACCGCGCCACAGC‑TTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTAt                       <  1:905677/140‑1 (MQ=255)                                                                                                             cGGAGCACGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTAt                       <  2:326558/140‑1 (MQ=255)                                                                                                                   aCGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACt                                   >  1:657502/1‑122 (MQ=255)                                                                                                                   aCGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACt                                   <  2:657502/122‑1 (MQ=255)                                                                                                                   aCGCGGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGt                 <  1:280304/140‑1 (MQ=255)                                                                                                                    cgcgGTGAAATTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTa                <  2:246347/140‑1 (MQ=255)                                                                                                                             aTTCGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTc       <  2:510934/140‑1 (MQ=255)                                                                                                                                cGGTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACg    <  1:754173/140‑1 (MQ=255)                                                                                                                                  gTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGcc  <  1:937855/140‑1 (MQ=255)                                                                                                                                  gTACTTCAGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGcc  <  1:394818/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCC  >  NC_000913/713556‑713824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑