Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,120,107	C→A	P81P (CCG→CCT)	menA ←	1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,120,107	0	C	A	100.0%	126.0 / NA	37	P81P (CCG→CCT)	menA	1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/19);  total (18/19)

CCGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGCGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGTGACCTTGCCAC  >  NC_000913/4119973‑4120242                                                                                                                                       |                                                                                                                                        ccGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGccc                                                                                                                                    >  2:143983/1‑140 (MQ=255) ccGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGccc                                                                                                                                    >  2:393094/1‑140 (MQ=255) ccGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGccc                                                                                                                                    >  1:369315/1‑140 (MQ=255) ccGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGccc                                                                                                                                    >  2:447938/1‑140 (MQ=255)    aCAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAAt                                                                                                                                 >  2:246897/1‑140 (MQ=255)                            gCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCttt                                                                                                         >  2:171849/1‑140 (MQ=255)                            gCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCttt                                                                                                         >  2:54674/1‑140 (MQ=255)                            gCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCttt                                                                                                         >  1:440713/1‑140 (MQ=255)                                   cAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCa                                                                                                  <  2:369315/140‑1 (MQ=255)                                   cAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCa                                                                                                  <  2:109470/140‑1 (MQ=255)                                   cAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCa                                                                                                  <  1:54674/140‑1 (MQ=255)                                   cAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCa                                                                                                  <  2:440713/140‑1 (MQ=255)                                   cAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCa                                                                                                  <  1:447938/140‑1 (MQ=255)                                    aaCCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCAt                                                                                                 <  1:246897/140‑1 (MQ=255)                                          tGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGt                                                                                           <  1:143983/140‑1 (MQ=255)                                          tGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGt                                                                                           <  1:171849/140‑1 (MQ=255)                                               gCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAAtaa                                                                                      >  1:403725/1‑138 (MQ=255)                                                    gagagaCAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGc                                                                                 <  1:285788/140‑1 (MQ=255)                                                               gAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGaaa                                                                      <  2:249974/140‑1 (MQ=255)                                                                  cACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGt                                                                            >  2:169296/1‑131 (MQ=255)                                                                  cACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGt                                                                            <  1:169296/131‑1 (MQ=255)                                                                  cACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGa                                                                   >  2:547752/1‑140 (MQ=255)                                                                        ggTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTa                                                             >  1:266453/1‑140 (MQ=255)                                                                          taataaTGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAAt                                                           >  1:232989/1‑140 (MQ=255)                                                                            ataatGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCATTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAg                                                         >  1:159795/1‑140 (MQ=255)                                                                                tGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCcg                                                     <  1:250172/140‑1 (MQ=255)                                                                                       ccGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTaa                                              <  1:477971/140‑1 (MQ=255)                                                                                                                 ccccTTTTTGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATAATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGa                    <  2:403725/140‑1 (MQ=255)                                                                                                                     tttttGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAAAAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGa                >  2:462432/1‑140 (MQ=255)                                                                                                                      ttttGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGaa               >  2:284186/1‑140 (MQ=255)                                                                                                                       tttGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAg              >  2:566564/1‑140 (MQ=255)                                                                                                                        ttGCATGCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGt             <  1:284186/140‑1 (MQ=255)                                                                                                                              gCCGCGTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGTGACCtt       <  2:240106/140‑1 (MQ=255)                                                                                                                                  cgTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCg                 >  1:456876/1‑126 (MQ=255)                                                                                                                                  cgTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCg                 <  2:456876/126‑1 (MQ=255)                                                                                                                                  cgTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGTGACCTTGcca   <  2:266453/140‑1 (MQ=255)                                                                                                                                   gTAGAGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGTGACCTTGccac  <  1:488470/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CCGACAAAATCGGCCAGCGTATGGCATGCCACTGCAACCAGTGCCAGCCCGGAGAGACAGATGAGCACGACGGTAATAATGAGCGCCCGTTTCATCTCTTGCTGGGTAATGACCCCTTTTTGCATGCCGCGTAGCGGCCCAATGCGGTCAGGTTTATCGCTGCCTTTTACCGCATCGCCGTAATCATTGGCGAGGTTAGAAAGGATCTGTAATAGCCCGGCGGTAATTAGTGCCAGCAGGGCGACCAGCGGATCGAAGTGACCTTGCCAC  >  NC_000913/4119973‑4120242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑