Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	786,247	G→T	A205A (GCG→GCT)	aroG →	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	786,247	0	G	T	100.0%	99.5 / NA	30	A205A (GCG→GCT)	aroG	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/21);  total (9/21)

GCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAA  >  NC_000913/786111‑786351                                                                                                                                         |                                                                                                         gcgcAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCAGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGctccg                                                                                                       >  2:537378/1‑140 (MQ=255)  cgcAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTTTCGATGCCATTAATGCCGCCGGTGctccgc                                                                                                      <  1:482660/140‑1 (MQ=255)    cAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCAc                                                                                                    >  2:433303/1‑140 (MQ=255)          gcgcACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCAGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTc                                                                                              <  1:537378/140‑1 (MQ=255)            gcACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCt                                                                                            <  2:158122/140‑1 (MQ=255)            gcACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCt                                                                                            <  1:523417/140‑1 (MQ=255)               cGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTc                                                                                         >  1:480734/1‑140 (MQ=255)                     accGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAAc                                                                                   <  1:567045/140‑1 (MQ=255)                         aaTCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGaaa                                                                               >  1:379027/1‑140 (MQ=255)                                 gTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCa                                                                       <  1:269436/140‑1 (MQ=255)                                      ccGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCgg                                                                  <  2:126525/140‑1 (MQ=255)                                        gcgAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCg                                                                <  2:467261/140‑1 (MQ=255)                                         cgAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGa                                                               >  1:165100/1‑140 (MQ=255)                                         cgAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGa                                                               <  2:173999/140‑1 (MQ=255)                                         cgAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGa                                                               <  1:69230/140‑1 (MQ=255)                                         cgAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGa                                                               <  2:94546/140‑1 (MQ=255)                                                                   cGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGAt                                     <  2:528217/140‑1 (MQ=255)                                                                      tCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGc                                  >  2:219889/1‑140 (MQ=255)                                                                      tCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGc                                  >  2:231331/1‑140 (MQ=255)                                                                      tCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGc                                  >  2:314594/1‑140 (MQ=255)                                                                       cGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGcc                                 <  1:314594/140‑1 (MQ=255)                                                                       cGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGcc                                 <  1:231331/140‑1 (MQ=255)                                                                          cTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCata                              >  2:124490/1‑140 (MQ=255)                                                                                    ggCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTgcg                    <  1:529301/140‑1 (MQ=255)                                                                                    ggCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTgcg                    >  2:529301/1‑140 (MQ=255)                                                                                    ggCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTgcg                    <  1:219889/140‑1 (MQ=255)                                                                                             ggTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTaa           <  1:433303/140‑1 (MQ=255)                                                                                               tACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAag         <  2:379027/140‑1 (MQ=255)                                                                                               tACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAag         <  1:124490/140‑1 (MQ=255)                                                                                               tACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAag         <  1:26237/140‑1 (MQ=255)                                                                                               tACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAag         <  2:95046/140‑1 (MQ=255)                                                                                                      aaaGTGGCTATCGATGCCATTAATGCCGCCGGTGCTCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTaa  <  2:480734/140‑1 (MQ=255)                                                                                                                                         |                                                                                                         GCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGGTACGATTAAAGTGGCTATCGATGCCATTAATGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAA  >  NC_000913/786111‑786351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑