Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA25.6% 83.1 / 11.2 43intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/9);  new base A (0/11);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 2.92e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCG  >  NC_000913/752986‑753263
                                                                                                                                           |                                                                                                                                          
gggTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCatat                                                                                                                                            <  1:1277651/140‑1 (MQ=255)
   ttATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCAtata                                                                                                                                           >  1:318898/1‑138 (MQ=255)
   ttATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCAtata                                                                                                                                           <  2:318898/138‑1 (MQ=255)
           gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                                                 >  2:824953/1‑140 (MQ=255)
           gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                                                 >  2:1288916/1‑140 (MQ=255)
             gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                               >  1:328795/1‑140 (MQ=255)
              aCTACCATGAATTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTTTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGc                                                                                                                              <  2:161247/140‑1 (MQ=255)
                         ttttttAATTGAAAGTATTGGGTTGCTGATAATTTGAGTTGTTCTATTTTTTTTAAATTTTTATATAGGTCTTTTAATGGATTTTTTTTTTAAAATTTTTTGTTTTTAGGCATATAAAAATCACCCCGCCATATGAAcgg                                                                                                                   <  2:97691/139‑1 (MQ=255)
                         gTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcgg                                                                                                                   >  2:367229/1‑140 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAat                                                                                                        >  2:1363267/1‑140 (MQ=255)
                                      aGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAatat                                                                                                      >  1:713888/1‑140 (MQ=255)
                                       gTATTGGTTTGCTAATAATTTGAGCTTTTCTTTTTTTTTAAAATATCTATATAGGTCTTTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGGAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATAtt                                                                                                     <  2:1022451/140‑1 (MQ=255)
                                         tttGGTTTGCTAATATTTTGAGCTTTTTTTTTTTTTTTAAATATTTATATGGGTTTGTTAATGTTTTTTTTTTTTAAATTTTTTTGTGTTTAGAAATAAAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTa                                                                                                   <  2:713888/139‑1 (MQ=255)
                                                tGCTGATAATTTGAGCTTTTCTATTCTTTTTAAATATCTATATAGGTTTTTTAATGGATTTTTTTTTTACATTTTTTTGTTTTTAGGAATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTa                                                                                            <  1:824953/140‑1 (MQ=255)
                                                 ggtaaTAATTTGATTTTTTTTTTTTTTTTTAAATATTTATATGGTTCTTTTAATGGTTTTTATTTTTACATTTTTTTGTGTTTGGCCAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                           <  2:132510/136‑1 (MQ=255)
                                                 gCTGATAATTTGAGCTTTTCTATTTTTTTTAAATATCTATATAGGTTTGTTAATGTATTTTATTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                           <  2:31230/140‑1 (MQ=255)
                                                       attttGAGTTTTTTTTTTTTTTTTAAATATTTATATGGGTTTGTAAATGATTTTTATTTTAAAATTTTTTTTTGTTTAGGAAAAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCa                                                                                     <  2:606907/138‑1 (MQ=255)
                                                            gAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                                >  1:1379417/1‑140 (MQ=255)
                                                                  ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGTGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGc                                                                          >  2:1202319/1‑140 (MQ=255)
                                                                         ttttttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                   <  1:391050/139‑1 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTATTGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  1:559711/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatctgtc                                                                >  1:921925/1‑135 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  1:1288009/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  1:1058276/1‑140 (MQ=255)
                                                                                      tatataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTc                                                      >  2:590533/1‑140 (MQ=255)
                                                                                            ggTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttt                                                >  2:104264/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                            >  2:174451/1‑140 (MQ=255)
                                                                                                 tttAATGGTTTTTATTTTTAAATTTTTTTGTGTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                           <  2:1402288/139‑1 (MQ=255)
                                                                                                 gTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                           <  1:367229/140‑1 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTATAACTTAGCAATCAACCATTAACGCTTGATATCGCCTTTAAAGTCGCGTTTTTCata                                          >  2:1285916/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  2:494612/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  2:47815/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  2:368097/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  2:377054/1‑140 (MQ=255)
                                                                                                    tttGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  2:559711/138‑1 (MQ=255)
                                                                                                    aaTGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  1:1363267/140‑1 (MQ=255)
                                                                                                    aaTGGTTTTTATTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  1:596101/140‑1 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGTGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  1:1202319/140‑1 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTAAATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  1:473382/140‑1 (MQ=255)
                                                                                                       ggATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                                     >  1:121433/1‑140 (MQ=255)
                                                                                                         tttttttttttAAATTTTTTTGTTTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCGACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGta                                   <  2:121433/139‑1 (MQ=255)
                                                                                                            ttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAc                                >  2:1136706/1‑140 (MQ=255)
                                                                                                                             tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc               <  1:368097/140‑1 (MQ=255)
                                                                                                                             tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc               <  1:1136706/140‑1 (MQ=255)
                                                                                                                              gtgtTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCa              <  1:1288916/140‑1 (MQ=255)
                                                                                                                               tgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCaa             <  1:377054/140‑1 (MQ=255)
                                                                                                                                    aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTc        >  1:16698/1‑140 (MQ=255)
                                                                                                                                     ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCa       <  1:494612/140‑1 (MQ=255)
                                                                                                                                          ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCg  <  2:16698/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCG  >  NC_000913/752986‑753263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑