Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,225	C→A	A41S (GCC→TCC)	nuoK ←	NADH:quinone oxidoreductase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,225	0	C	A	100.0%	213.9 / NA	61	A41S (GCC→TCC)	nuoK	NADH:quinone oxidoreductase subunit K
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/33);  total (28/33)

TGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCC  >  NC_000913/2395095‑2395354                                                                                                                                   |                                                                                                                                  tGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCAt                                                                                                                          <  1:582610/140‑1 (MQ=255) tGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCAt                                                                                                                          <  1:1035928/140‑1 (MQ=255)      ttGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGAttt                                                                                                                     <  2:307647/140‑1 (MQ=255)        gcagcagcaGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTaa                                                                                                                              >  1:1296015/1‑129 (MQ=255)        gcagcagcaGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTaa                                                                                                                              <  2:1296015/129‑1 (MQ=255)        gcagcagcaGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATg                                                                                                                         >  2:899180/1‑134 (MQ=255)        gcagcagcaGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATg                                                                                                                         <  1:899180/134‑1 (MQ=255)        gcagcagcaGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTcc                                                                                                                   >  1:1217466/1‑140 (MQ=255)         cagcagcagCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCa                                                                                                                  <  1:191455/140‑1 (MQ=255)         cagcagcagCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCa                                                                                                                  <  2:695486/140‑1 (MQ=255)           gcagcagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGAttt                                                                                                                     >  2:1380421/1‑135 (MQ=255)           gcagcagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGAttt                                                                                                                     <  1:1380421/135‑1 (MQ=255)           gcagcagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGa                                                                                                                <  2:1217466/140‑1 (MQ=255)              gcagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCa                                                                                                             >  1:192581/1‑140 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            >  2:240806/1‑140 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            >  1:43728/1‑140 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            <  2:913914/140‑1 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            <  2:41675/140‑1 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            >  2:986603/1‑140 (MQ=255)               cagcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCaa                                                                                                            >  2:1170742/1‑140 (MQ=255)                agcGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAAt                                                                                                           <  1:138802/140‑1 (MQ=255)                 gcgcAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATc                                                                                                          <  2:521604/140‑1 (MQ=255)                  cgcAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGTCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCa                                                                                                         <  2:1267528/140‑1 (MQ=255)                  cgcAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCa                                                                                                         <  2:160963/140‑1 (MQ=255)                  cgcAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCa                                                                                                         <  1:721242/140‑1 (MQ=255)                      aGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACAt                                                                                                     >  1:208984/1‑140 (MQ=255)                            aTACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAAcag                                                                                               <  1:240806/140‑1 (MQ=255)                               cTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAAcagcag                                                                                            <  1:741817/140‑1 (MQ=255)                                  gCTTCTGCCGCCGCGAGACTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGAtt                                                                                         >  1:1411282/1‑140 (MQ=255)                                    ttCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGc                                                                                       <  2:43728/140‑1 (MQ=255)                                    ttCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGc                                                                                       <  2:208984/140‑1 (MQ=255)                                       tGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGAc                                                                                    >  2:1002487/1‑140 (MQ=255)                                         ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACgg                                                                                  >  1:103979/1‑140 (MQ=255)                                                 aGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAg                                                                          <  2:103979/140‑1 (MQ=255)                                                    cTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGAcc                                                                       <  1:1002487/140‑1 (MQ=255)                                                               aaTGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCaa                                                            <  2:192581/140‑1 (MQ=255)                                                                 tGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGa                                                          <  1:1170742/140‑1 (MQ=255)                                                                    aCATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAAct                                                       <  2:264579/140‑2 (MQ=255)                                                                         aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATaa                                                  >  1:633404/1‑140 (MQ=255)                                                                           cTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGa                                                >  2:520432/1‑140 (MQ=255)                                                                             gACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGAtt                                              >  2:866299/1‑140 (MQ=255)                                                                             gACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGAtt                                              >  2:988941/1‑140 (MQ=255)                                                                              aCCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTg                                             >  2:1259769/1‑140 (MQ=255)                                                                                   cGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCcgcg                                        >  1:258172/1‑140 (MQ=255)                                                                                   cGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCcgcg                                        <  1:1259769/140‑1 (MQ=255)                                                                                   cGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCcgcg                                        >  1:1062545/1‑140 (MQ=255)                                                                                       cTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGa                                    <  1:988941/140‑1 (MQ=255)                                                                                       cTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGa                                    <  1:986603/140‑1 (MQ=255)                                                                                         ggCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATc                                  <  1:520432/140‑1 (MQ=255)                                                                                               aGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCa                            >  1:922850/1‑140 (MQ=255)                                                                                               aGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCa                            >  1:777026/1‑140 (MQ=255)                                                                                               aGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCa                            >  1:1012601/1‑140 (MQ=255)                                                                                                 tAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCAtg                          <  1:866299/140‑1 (MQ=255)                                                                                                   gCTTCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCAtgtt                        <  2:704721/140‑1 (MQ=255)                                                                                                      tCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTa                     >  2:398685/1‑140 (MQ=255)                                                                                                      tCCGGCGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTa                     >  2:430716/1‑140 (MQ=255)                                                                                                           cGACCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAgggg                >  1:1257974/1‑140 (MQ=255)                                                                                                             aCCACGAAGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGAt              >  1:289439/1‑140 (MQ=255)                                                                                                               cACGAAGGCCAACGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCa            >  2:1293762/1‑140 (MQ=255)                                                                                                                   aaGGCCAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCg        <  1:271637/140‑1 (MQ=255)                                                                                                                        cAGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGctc   <  2:920059/140‑1 (MQ=255)                                                                                                                         aGCGCGGAGGAGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGctcc  >  1:9947/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                  TGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCC  >  NC_000913/2395095‑2395354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑