Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,995,552	C→A	A264E (GCG→GAG)	dapF →	diaminopimelate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,995,552	0	C	A	100.0%	232.0 / NA	66	A264E (GCG→GAG)	dapF	diaminopimelate epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/22);  total (44/22)

GCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATGCG  >  NC_000913/3995417‑3995687                                                                                                                                        |                                                                                                                                        gcggcgCGTGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTa                                                                                                                                     <  2:1291555/140‑1 (MQ=255)  cggcgCGTGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTAc                                                                                                                                    <  1:1395291/140‑1 (MQ=255)  cggcgCGTGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTAc                                                                                                                                    <  1:332183/140‑1 (MQ=255)            gcggcgGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTacga                                                                                                                          <  2:296818/140‑1 (MQ=255)                                tCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGaa                                                                                                      >  2:1406951/1‑140 (MQ=255)                                tCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGaa                                                                                                      >  2:1284754/1‑140 (MQ=255)                                 cAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAg                                                                                                     <  1:249738/140‑1 (MQ=255)                                   gCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCa                                                                                                   >  1:722477/1‑140 (MQ=255)                                      aGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAAcc                                                                                                >  2:160620/1‑140 (MQ=255)                                              tGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAgggg                                                                                           <  1:609760/137‑1 (MQ=255)                                              tGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAgggg                                                                                           >  2:609760/1‑137 (MQ=255)                                                        agTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGa                                                                              <  2:564224/140‑1 (MQ=255)                                                            cgcgTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAaca                                                                          >  1:341192/1‑140 (MQ=255)                                                              cgTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACt                                                                        >  2:41351/1‑140 (MQ=255)                                                                tGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCa                                                                      <  1:1284754/140‑1 (MQ=255)                                                                tGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCa                                                                      <  2:722477/140‑1 (MQ=255)                                                                tGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCa                                                                      <  2:216532/140‑1 (MQ=255)                                                                   aaCTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACgg                                                                   <  1:249563/140‑1 (MQ=255)                                                                   aaCTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACgg                                                                   >  2:249563/1‑140 (MQ=255)                                                                    aCTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGa                                                                  >  1:1302107/1‑140 (MQ=255)                                                                        cccGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCtt                                                              >  1:975477/1‑140 (MQ=255)                                                                                  gtcTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCggg                                                    >  2:443862/1‑140 (MQ=255)                                                                                  gtcTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCggg                                                    <  2:341192/140‑1 (MQ=255)                                                                                      tGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGt                                                <  1:1406951/140‑1 (MQ=255)                                                                                                  gAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACTggg                                                    >  2:1141299/1‑124 (MQ=255)                                                                                                  gAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACTggg                                                    <  1:1141299/124‑1 (MQ=255)                                                                                                  gAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCt                                    >  2:112654/1‑140 (MQ=255)                                                                                                            ggTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTg                                   <  1:578402/131‑1 (MQ=255)                                                                                                            ggTCACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTg                                   >  2:578402/1‑131 (MQ=255)                                                                                                               cACCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCt                       <  1:443862/140‑1 (MQ=255)                                                                                                                aCCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTg                      <  1:1324319/140‑1 (MQ=255)                                                                                                                aCCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTg                      <  1:160620/140‑1 (MQ=255)                                                                                                                aCCCGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTg                      <  1:1184160/140‑1 (MQ=255)                                                                                                                 cccGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGa                     >  2:1237139/1‑140 (MQ=255)                                                                                                                  ccGTTATATATGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAg                    >  1:269339/1‑140 (MQ=255)                                                                                                                            tGACTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATcc          >  2:154972/1‑140 (MQ=255)                                                                                                                              aCTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGt        >  1:201766/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  1:1044984/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  2:1404474/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  2:448439/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  1:447699/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  2:775232/1‑140 (MQ=255)                                                                                                                               cTGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTa       >  1:234427/1‑140 (MQ=255)                                                                                                                                tGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAGCACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTaa      >  2:1251060/1‑140 (MQ=255)                                                                                                                                tGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTaa      >  2:1165024/1‑140 (MQ=255)                                                                                                                                tGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTaa      >  1:1059154/1‑140 (MQ=255)                                                                                                                                tGGCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTaa      >  2:287157/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGttttt               >  2:309751/1‑129 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGttttt               <  1:309751/129‑1 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:885683/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:628211/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:1007446/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:303614/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:1337365/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:1317949/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:1292031/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  2:1124138/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:978409/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    <  1:775232/140‑1 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:621752/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:52940/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:410991/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:272545/1‑140 (MQ=255)                                                                                                                                  gCCCGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATg    >  1:241804/1‑140 (MQ=255)                                                                                                                                   cccGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATgc   >  2:621787/1‑140 (MQ=255)                                                                                                                                    ccGGAGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATgcg  <  2:1007446/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTGGAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCTGAGTTTTTTATCCGTAATGCG  >  NC_000913/3995417‑3995687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑