Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,411,402	C→A	T34K (ACG→AAG)	yjfK →	conserved protein YjfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,411,402	0	C	A	100.0%	274.8 / NA	78	T34K (ACG→AAG)	yjfK	conserved protein YjfK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (48/30);  total (48/30)

AAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTT  >  NC_000913/4411271‑4411537                                                                                                                                    |                                                                                                                                        aaaaGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGcgt                                                                                                                                 <  2:405872/140‑1 (MQ=255)        tGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGcgtttcgttt                                                                                                                          >  1:1165906/1‑140 (MQ=255)        tGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGcgtttcgttt                                                                                                                          >  2:716877/1‑140 (MQ=255)          gCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGc                                                                                                                        <  2:916248/140‑1 (MQ=255)                     aaGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGaa                                                                                                             <  1:766277/140‑1 (MQ=255)                      aGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAAt                                                                                                            >  2:754979/1‑140 (MQ=255)                        gATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTg                                                                                                          >  1:114294/1‑140 (MQ=255)                         aTAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGc                                                                                                                        <  1:524423/125‑1 (MQ=255)                         aTAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGc                                                                                                                        >  2:524423/1‑125 (MQ=255)                         aTAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGc                                                                                                         >  1:1002739/1‑140 (MQ=255)                         aTAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGc                                                                                                         >  2:745153/1‑140 (MQ=255)                           aaaaTATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTg                                                                                                       >  2:921772/1‑140 (MQ=255)                                     tGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGc                                                                                             <  1:716877/140‑1 (MQ=255)                                       gTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCg                                                                                           >  2:780275/1‑140 (MQ=255)                                       gTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCg                                                                                           >  1:859752/1‑140 (MQ=255)                                       gTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCg                                                                                           >  1:1102181/1‑140 (MQ=255)                                        ttttttCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCgg                                                                                          >  1:251571/1‑140 (MQ=255)                                        ttttttCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCgg                                                                                          >  2:144636/1‑140 (MQ=255)                                            ttCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGa                                                                                      <  2:251571/140‑1 (MQ=255)                                             tCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAg                                                                                     >  2:684770/1‑140 (MQ=255)                                             tCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAg                                                                                     <  2:1002739/140‑1 (MQ=255)                                                 gCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGa                                                                                      >  2:354868/1‑135 (MQ=255)                                                 gCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGa                                                                                      <  1:354868/135‑1 (MQ=255)                                                 gCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAAt                                                                                 >  1:544378/1‑140 (MQ=255)                                                          tGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAg                                                                        <  1:780275/140‑1 (MQ=255)                                                           ggCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAgc                                                                       <  1:745153/140‑1 (MQ=255)                                                               aGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAgccgcc                                                                   <  2:114294/140‑1 (MQ=255)                                                                  ataataAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTc                                                                >  1:8996/1‑140 (MQ=255)                                                                    aataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAg                                                              <  1:636934/140‑1 (MQ=255)                                                                    aataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAg                                                              <  2:1102181/140‑1 (MQ=255)                                                                    aataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAg                                                              <  2:1071016/140‑1 (MQ=255)                                                                         gCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCcaca                                                         <  1:684770/140‑1 (MQ=255)                                                                           cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATc                                                       >  1:412239/1‑140 (MQ=255)                                                                           cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGCCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAgccgcc                                                                   <  1:541377/128‑1 (MQ=255)                                                                           cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGCCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAgccgcc                                                                   >  2:541377/1‑128 (MQ=255)                                                                              cTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGAt                                                    >  2:984762/1‑140 (MQ=255)                                                                              cTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGAt                                                    >  2:987326/1‑140 (MQ=255)                                                                              cTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGAt                                                    >  1:946638/1‑140 (MQ=255)                                                                                aTCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCt                                                  >  2:1281455/1‑140 (MQ=255)                                                                                   gCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTggg                                               >  2:787879/1‑140 (MQ=255)                                                                                   gCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTggg                                               >  2:1279361/1‑140 (MQ=255)                                                                                    cTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGggc                                              >  2:709963/1‑140 (MQ=255)                                                                                      cGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGAt                                                    <  1:331329/132‑1 (MQ=255)                                                                                      cGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGAt                                                    >  2:331329/1‑132 (MQ=255)                                                                                        tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGgcggcg                                          <  1:709963/140‑1 (MQ=255)                                                                                        tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGgcggcg                                          >  1:663646/1‑140 (MQ=255)                                                                                           tCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTa                                       >  1:1259724/1‑140 (MQ=255)                                                                                             cGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGt                                     >  1:1057953/1‑140 (MQ=255)                                                                                             cGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGt                                     >  2:1091170/1‑140 (MQ=255)                                                                                             cGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGt                                     >  2:801352/1‑140 (MQ=255)                                                                                             cGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGt                                     >  1:494730/1‑140 (MQ=255)                                                                                               cTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCa                                   >  1:1049826/1‑140 (MQ=255)                                                                                               cTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCa                                   <  1:754979/140‑1 (MQ=255)                                                                                                  ggACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGggcggc                                           <  1:1034616/129‑1 (MQ=255)                                                                                                  ggACTTCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGggcggc                                           >  2:1034616/1‑129 (MQ=255)                                                                                                      ttCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                            <  2:1049826/140‑1 (MQ=255)                                                                                                      ttCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                            <  1:144636/140‑1 (MQ=255)                                                                                                      ttCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                            <  1:921772/140‑1 (MQ=255)                                                                                                       tCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTcc                           <  2:544378/140‑1 (MQ=255)                                                                                                       tCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                            >  1:1021130/1‑139 (MQ=255)                                                                                                       tCATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                            <  2:1021130/139‑1 (MQ=255)                                                                                                        cATCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCg                          >  2:52440/1‑140 (MQ=255)                                                                                                         aTCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                         <  1:52440/140‑1 (MQ=255)                                                                                                         aTCTCAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCATTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                         >  2:1267058/1‑140 (MQ=255)                                                                                                          tctcAATAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGct                        >  2:458395/1‑140 (MQ=255)                                                                                                              aaTAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGctacta                    <  2:1259724/140‑1 (MQ=255)                                                                                                              aaTAGTGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTACCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGctacta                    <  2:859752/140‑1 (MQ=255)                                                                                                                   tGGCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt               >  2:914948/1‑140 (MQ=255)                                                                                                                     gCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCa             >  2:654608/1‑140 (MQ=255)                                                                                                                     gCTTTACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCa             >  2:686193/1‑140 (MQ=255)                                                                                                                        ttACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                         <  2:505268/125‑1 (MQ=255)                                                                                                                        ttACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                         >  1:505268/1‑125 (MQ=255)                                                                                                                         tACGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCg         >  2:577971/1‑140 (MQ=255)                                                                                                                          aCGCTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGa        >  1:354161/1‑140 (MQ=255)                                                                                                                             cTCGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGa     >  2:73947/1‑140 (MQ=255)                                                                                                                               cGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGAt       <  1:1224099/136‑1 (MQ=255)                                                                                                                               cGACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGAt       >  2:1224099/1‑136 (MQ=255)                                                                                                                                gACAAGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGtt  >  2:260345/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        AAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTT  >  NC_000913/4411271‑4411537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑