Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,645,663	C→A	K70N (AAG→AAT)	relB ←	Qin prophage; antitoxin/DNA‑binding transcriptional repressor RelB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,645,663	0	C	A	100.0%	247.3 / NA	70	K70N (AAG→AAT)	relB	Qin prophage; antitoxin/DNA‑binding transcriptional repressor RelB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/43);  total (27/43)

ACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCTTCAGAAGGAGT  >  NC_000913/1645528‑1645797                                                                                                                                        |                                                                                                                                        aCTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAg                                                                                                                                     <  1:505862/140‑1 (MQ=255)  cTCAAGCACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAgg                                                                                                                                    >  2:986341/1‑140 (MQ=255)      aGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTggattaggatta                                                                                                                                >  1:1003934/1‑140 (MQ=255)           ttCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAg                                                                                                                           >  1:325554/1‑140 (MQ=255)            tCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGc                                                                                                                          <  2:603994/140‑1 (MQ=255)            tCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGc                                                                                                                          <  1:1252237/140‑1 (MQ=255)            tCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGc                                                                                                                          <  2:366408/140‑1 (MQ=255)             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGcc                                                                                                                         >  1:98115/1‑140 (MQ=255)             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGcc                                                                                                                         <  2:276940/140‑1 (MQ=255)             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGcc                                                                                                                         <  2:1003934/140‑1 (MQ=255)             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGcc                                                                                                                         <  1:321581/140‑1 (MQ=255)              aaCCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCg                                                                                                                        <  2:985692/140‑1 (MQ=255)                ccAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGtt                                                                                                                      <  2:325554/140‑1 (MQ=255)                   gCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCtt                                                                                                                   >  2:832189/1‑140 (MQ=255)                      tcttTTTCAACTGTTCCCGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCa                                                                                                                <  2:979644/140‑1 (MQ=255)                      tcttTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCa                                                                                                                <  1:1476711/140‑1 (MQ=255)                      tcttTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCa                                                                                                                <  2:55916/140‑1 (MQ=255)                        tttttCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCTATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACt                                                                                                              <  2:1453681/140‑1 (MQ=255)                           ttCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTAtc                                                                                                           <  1:1544755/140‑1 (MQ=255)                              aaCTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTcc                                                                                                        >  1:1561007/1‑140 (MQ=255)                              aaCTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTcc                                                                                                        <  2:679813/140‑1 (MQ=255)                                 tGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCcaca                                                                                                     <  1:915035/140‑1 (MQ=255)                                         tACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGc                                                                                             <  1:620330/140‑1 (MQ=255)                                          aCCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCa                                                                                            >  1:1367506/1‑140 (MQ=255)                                             gTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCt                                                                                         >  2:649830/1‑140 (MQ=255)                                               cGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtc                                                                                       >  2:1356916/1‑140 (MQ=255)                                                gAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca                                                                                      <  2:98115/140‑1 (MQ=255)                                                gAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca                                                                                      <  1:1406970/140‑1 (MQ=255)                                                  gCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtcatc                                                                                    >  1:1380324/1‑140 (MQ=255)                                                  gCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca                                                                                      >  1:70694/1‑138 (MQ=255)                                                  gCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca                                                                                      <  2:70694/138‑1 (MQ=255)                                                   cccAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtcatca                                                                                   >  2:559085/1‑140 (MQ=255)                                                     cAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACt                                                                                 <  1:458870/140‑1 (MQ=255)                                                                aTTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCt                                                                     <  2:1137487/140‑1 (MQ=255)                                                                        aGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAGCTGTCtcttatac                                                              <  1:832189/140‑8 (MQ=255)                                                                        aGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAAc                                                             <  2:758307/140‑1 (MQ=255)                                                                           gCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGc                                                          <  2:269889/140‑1 (MQ=255)                                                                            cccGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCa                                                         <  1:649830/140‑1 (MQ=255)                                                                            cccGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCa                                                         <  1:986341/140‑1 (MQ=255)                                                                              cGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAg                                                       >  1:1418334/1‑140 (MQ=255)                                                                              cGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAg                                                       >  2:1369716/1‑140 (MQ=255)                                                                              cGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAg                                                       >  2:1343907/1‑140 (MQ=255)                                                                              cGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAg                                                       >  1:1383155/1‑140 (MQ=255)                                                                               gCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGc                                                      >  1:773834/1‑140 (MQ=255)                                                                                cTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCg                                                     >  2:1448143/1‑140 (MQ=255)                                                                                    tCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCa                                                         >  2:445291/1‑132 (MQ=255)                                                                                    tCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCa                                                         <  1:445291/132‑1 (MQ=255)                                                                                    tCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCa                                                 >  1:937757/1‑140 (MQ=255)                                                                                      aaaaTCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCAtt                                               <  1:1343907/140‑1 (MQ=255)                                                                                        aaTCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGt                                             <  2:1561007/140‑1 (MQ=255)                                                                                        aaTCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGt                                             <  2:937757/140‑1 (MQ=255)                                                                                         aTCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTc                                            <  1:559085/140‑1 (MQ=255)                                                                                           ccAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAg                                          <  1:1356916/140‑1 (MQ=255)                                                                                               aaaaTACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGat                                      <  2:1418334/140‑1 (MQ=255)                                                                                                    aCGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACt                                 <  1:1421741/140‑1 (MQ=255)                                                                                                    aCGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACt                                 <  1:147423/140‑1 (MQ=255)                                                                                                     cGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTc                                <  2:1380324/140‑1 (MQ=255)                                                                                                     cGCCATCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTc                                >  1:83512/1‑140 (MQ=255)                                                                                                         aTCAGAGTTCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGc                            >  1:1155198/1‑140 (MQ=255)                                                                                                                 tCATCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACg                    <  1:1448143/140‑1 (MQ=255)                                                                                                                    tCCAGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 >  1:18306/1‑140 (MQ=255)                                                                                                                       aGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAgcgc              <  2:83512/140‑1 (MQ=255)                                                                                                                       aGCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAgcgc              <  2:18306/140‑1 (MQ=255)                                                                                                                        gCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 <  1:1105410/136‑1 (MQ=255)                                                                                                                        gCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 >  2:1105410/1‑136 (MQ=255)                                                                                                                        gCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 >  1:249519/1‑136 (MQ=255)                                                                                                                        gCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 <  2:249519/136‑1 (MQ=255)                                                                                                                        gCGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCt             >  2:1495102/1‑140 (MQ=255)                                                                                                                         cGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 <  1:1321340/135‑1 (MQ=255)                                                                                                                         cGTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAg                 >  2:1321340/1‑135 (MQ=255)                                                                                                                          gTCACACGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCTTc           >  1:477428/1‑140 (MQ=255)                                                                                                                              cacGTACTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCTTCAGaa       >  2:1608955/1‑140 (MQ=255)                                                                                                                                   aCTGGATTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCTTCAGAAGGAGt  >  1:138466/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCATCACTCAGGAG‑TGTCTGTTTGAACGGCAAGCGTTCATTGTCAGCGATATACTCGAGCATGAGACGAAGCGCTTCAGAAGGAGT  >  NC_000913/1645528‑1645797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑