Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,838	G→T	intergenic (+540/‑2441)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,838	0	G	T	100.0%	316.5 / NA	90	intergenic (+540/‑2441)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/46);  total (44/46)

ATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCG  >  NC_000913/2197703‑2197974                                                                                                                                        |                                                                                                                                         aTTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCa                                                                                                                                      >  2:327611/1‑140 (MQ=255)   taacacTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    <  2:1388330/138‑1 (MQ=255)   tGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    <  2:1101569/140‑1 (MQ=255)       acTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATgt                                                                                                                                <  2:1515604/140‑2 (MQ=255)       acTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGc                                                                                                                                <  2:126847/140‑1 (MQ=255)         tGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTa                                                                                                                              >  2:870196/1‑140 (MQ=255)         tGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTa                                                                                                                              >  2:828323/1‑140 (MQ=255)          gCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTaa                                                                                                                             <  2:897711/140‑1 (MQ=255)          gCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTaa                                                                                                                             <  2:819224/140‑1 (MQ=255)           cTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTAt                                                                                                                                  <  1:1218567/134‑1 (MQ=255)           cTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTAt                                                                                                                                  >  2:1218567/1‑134 (MQ=255)           cTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAAt                                                                                                                            >  2:718951/1‑140 (MQ=255)                   tgtAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGc                                                                                                                    >  1:641925/1‑140 (MQ=255)                   tgtAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGc                                                                                                                    >  2:1339750/1‑140 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    <  1:530841/122‑1 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    >  2:119646/1‑122 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    >  2:530841/1‑122 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCAtt                                                                                                                                    <  1:119646/122‑1 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCt                                                                                                                               <  2:831480/127‑1 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCt                                                                                                                               >  1:831480/1‑127 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCtt                                                                                                                  >  2:1242463/1‑140 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCtt                                                                                                                  >  2:996680/1‑140 (MQ=255)                     tAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCtt                                                                                                                  >  1:508318/1‑140 (MQ=255)                     tAGCCGGACAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCtt                                                                                                                  >  1:607767/1‑140 (MQ=255)                       gCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTAt                                                                                                                >  1:260712/1‑140 (MQ=255)                            gCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGAc                                                                                                                         >  2:428797/1‑126 (MQ=255)                            gCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGAc                                                                                                                         <  1:428797/126‑1 (MQ=255)                            gCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTAtct                                                                                                              <  2:803267/137‑1 (MQ=255)                            gCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTAtct                                                                                                              >  1:803267/1‑137 (MQ=255)                            gCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGGTCCCACATGCAGCAATGGTGGCGCGGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCACATTATGCTAATGACAAAGCTTATCTcac                                                                                                           >  2:1096282/1‑140 (MQ=255)                              aaaCTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACaa                                                                                                         <  1:327611/140‑1 (MQ=255)                                   aaaCGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGc                                                                                                    <  2:1615569/140‑1 (MQ=255)                                   aaaCGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGc                                                                                                    <  1:870196/140‑1 (MQ=255)                                   aaaCGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGc                                                                                                    <  1:996680/140‑1 (MQ=255)                                      cGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACt                                                                                                 >  2:567242/1‑140 (MQ=255)                                        cTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTgg                                                                                               <  1:1029318/140‑1 (MQ=255)                                          gTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCa                                                                                             >  1:43656/1‑140 (MQ=255)                                           tCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTAGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAg                                                                                            >  2:1420582/1‑140 (MQ=255)                                            cACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGa                                                                                           >  2:293927/1‑140 (MQ=255)                                            cACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGa                                                                                           >  2:230954/1‑140 (MQ=255)                                             aCGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGaa                                                                                          <  2:641925/140‑1 (MQ=255)                                              cGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAAc                                                                                         <  1:230954/140‑1 (MQ=255)                                                  cGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGt                                                                                     <  1:1339750/140‑1 (MQ=255)                                                   ggATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTc                                                                                    >  2:1450786/1‑140 (MQ=255)                                                    gATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCa                                                                                   <  1:567242/140‑1 (MQ=255)                                                         cGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTccc                                                                              <  1:1350380/140‑1 (MQ=255)                                                         cGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAAAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTccc                                                                              >  1:196924/1‑140 (MQ=255)                                                            aaGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTgg                                                                           >  2:870974/1‑140 (MQ=255)                                                                  ttGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCg                                                                     >  1:303144/1‑140 (MQ=255)                                                                   tgctgcGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGa                                                                    >  2:859817/1‑140 (MQ=255)                                                                   tgctgcGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGa                                                                    >  2:1382797/1‑140 (MQ=255)                                                                          ttCCCACATGCAGTAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGc                                                             <  1:1242463/140‑1 (MQ=255)                                                                          ttCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGc                                                             >  2:1045985/1‑140 (MQ=255)                                                                            cccACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAg                                                           >  2:715053/1‑140 (MQ=255)                                                                            cccACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAg                                                           >  2:1445034/1‑140 (MQ=255)                                                                            cccACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAg                                                           <  1:1474254/140‑1 (MQ=255)                                                                             ccACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGt                                                          >  2:295605/1‑140 (MQ=255)                                                                                  tGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCg                                                     >  2:627528/1‑140 (MQ=255)                                                                                  tGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCg                                                     >  1:438320/1‑140 (MQ=255)                                                                                     agcaATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTAc                                                  >  1:1247464/1‑140 (MQ=255)                                                                                         aTGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGaa                                              >  1:1442488/1‑140 (MQ=255)                                                                                           ggcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAAt                                            <  2:1161131/140‑1 (MQ=255)                                                                                            gcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATc                                           >  1:1143479/1‑140 (MQ=255)                                                                                            gcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATc                                           >  1:1108828/1‑140 (MQ=255)                                                                                             cggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCa                                          >  1:1415911/1‑140 (MQ=255)                                                                                               gcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATg                                        <  2:303144/140‑1 (MQ=255)                                                                                                cACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGc                                       <  2:1415911/140‑1 (MQ=255)                                                                                                    gACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGc                                   <  1:870974/140‑1 (MQ=255)                                                                                                    gACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGc                                   <  2:607767/140‑1 (MQ=255)                                                                                                     aCGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGca                                  >  1:1365848/1‑140 (MQ=255)                                                                                                     aCGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGca                                  <  2:1365848/140‑1 (MQ=255)                                                                                                     aCGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGca                                  <  2:1143479/140‑1 (MQ=255)                                                                                                       ggAACTTCTTGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGcaac                                >  1:737461/1‑140 (MQ=255)                                                                                                            ttcttGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTg                           <  2:1108828/140‑1 (MQ=255)                                                                                                               ttGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGaca                        <  2:1442488/140‑1 (MQ=255)                                                                                                               ttGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGaca                        >  1:514005/1‑140 (MQ=255)                                                                                                               ttGGGCAAAAAGAAAAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGaca                        <  2:196924/140‑1 (MQ=255)                                                                                                                tGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAGATCATGCCAGCAACAACTGacac                       <  1:1341256/140‑1 (MQ=255)                                                                                                                tGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGacac                       <  1:431132/140‑1 (MQ=255)                                                                                                                tGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGacac                       <  1:828323/140‑1 (MQ=255)                                                                                                                tGGGCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGacac                       <  1:249292/140‑1 (MQ=255)                                                                                                                 gggCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGAcaca                      <  1:718951/140‑1 (MQ=255)                                                                                                                 gggCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGAcaca                      <  1:715053/140‑1 (MQ=255)                                                                                                                 gggCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGAcaca                      <  1:1445034/140‑1 (MQ=255)                                                                                                                 gggCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGAcaca                      <  1:859817/140‑1 (MQ=255)                                                                                                                 gggCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGAcaca                      <  1:1450786/140‑1 (MQ=255)                                                                                                                   gCAAAAAGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGc                    >  2:101870/1‑140 (MQ=255)                                                                                                                      aaaaGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCt                 <  2:773029/140‑1 (MQ=255)                                                                                                                      aaaaGAAGAGAACAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCt                 <  1:101870/140‑1 (MQ=255)                                                                                                                                aaCAGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGc       <  1:293927/140‑1 (MQ=255)                                                                                                                                   aGTTGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAg    <  2:1495172/140‑1 (MQ=255)                                                                                                                                     ttGTCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAgcg  >  2:1520776/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         ATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCCAGCAACAACTGACACAGCCCTCAAACCATGCCAGCG  >  NC_000913/2197703‑2197974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑