Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	98.4%	234.1 / ‑3.5	64	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (26/37);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815740‑3816012                                                                                                                                              |                                                                                                                                       tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat‑t‑c                                                                                                                                       <  1:310157/140‑1 (MQ=255)  cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat‑t‑c                                                                                                                                       <  2:1344116/140‑1 (MQ=255)      aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑ccccc                                                                                                                                   <  2:1470116/140‑2 (MQ=255)       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGTCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑ccccct                                                                                                                                  <  2:114511/140‑3 (MQ=255)       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑ccccct                                                                                                                                  >  2:813268/1‑138 (MQ=255)           ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCggg                                                                                                                               >  1:1010385/1‑140 (MQ=255)            gcgcTGATATGGTTTCATGTCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGc                                                                                                                              <  2:660550/140‑1 (MQ=255)            gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGGCTAAAAAATCAGTTGGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATGC‑CCCCTCGGGc                                                                                                                              <  2:997676/140‑1 (MQ=255)            gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGc                                                                                                                              <  1:715191/140‑1 (MQ=255)               cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCag                                                                                                                           >  1:457112/1‑140 (MQ=255)                    atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑Cca                                                                                                                     <  1:631812/140‑1 (MQ=255)                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATg                                                                                                             >  1:220181/1‑140 (MQ=255)                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATg                                                                                                             >  2:714225/1‑140 (MQ=255)                              gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAg                                                                                                           <  2:970815/140‑1 (MQ=255)                              gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAg                                                                                                           <  2:954777/140‑1 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAg                                                                                                           >  1:1191859/1‑137 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAg                                                                                                           <  2:1191859/137‑1 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        >  1:996046/1‑140 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        <  1:1618623/140‑1 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        <  1:714225/140‑1 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        <  2:209902/140‑1 (MQ=255)                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        <  2:685266/140‑1 (MQ=255)                                  tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        >  2:283272/1‑139 (MQ=255)                                  tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                        <  1:283272/139‑1 (MQ=255)                                  tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGc                                                                                                       <  1:1024303/140‑1 (MQ=255)                                  tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGc                                                                                                       >  2:1391160/1‑140 (MQ=255)                                      tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCtt                                                                                                   <  2:1010385/140‑1 (MQ=255)                                                aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                         <  2:894376/140‑1 (MQ=255)                                                    ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                     >  2:571993/1‑140 (MQ=255)                                                        aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                 >  2:484935/1‑140 (MQ=255)                                                         cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                <  2:996046/140‑1 (MQ=255)                                                           gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAGCCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                                                                               >  2:1399056/1‑140 (MQ=255)                                                           gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                              <  1:571993/140‑1 (MQ=255)                                                            aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  2:571350/1‑140 (MQ=255)                                                                aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    >  1:574920/1‑129 (MQ=255)                                                                aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    <  2:574920/129‑1 (MQ=255)                                                                      ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:482321/1‑140 (MQ=255)                                                                        cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1358071/140‑1 (MQ=255)                                                                        cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 >  2:1167886/1‑140 (MQ=255)                                                                        cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:484935/140‑1 (MQ=255)                                                                          aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  1:488037/1‑140 (MQ=255)                                                                          aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  1:1391160/140‑1 (MQ=255)                                                                          aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  1:1640142/1‑140 (MQ=255)                                                                          aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  2:1482437/1‑140 (MQ=255)                                                                                  gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                       >  2:31431/1‑140 (MQ=255)                                                                                     gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  1:1452562/1‑140 (MQ=255)                                                                                     gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    <  1:1482437/140‑1 (MQ=255)                                                                                     gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  1:299337/1‑140 (MQ=255)                                                                                          aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAGCCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCTGTCCCCTGCACTTCAATGATGCGcc                                                <  1:1399056/140‑1 (MQ=255)                                                                                                 aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:425173/1‑140 (MQ=255)                                                                                                 aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        <  1:1167886/140‑1 (MQ=255)                                                                                                   tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      >  1:1058687/1‑140 (MQ=255)                                                                                                   tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:31431/140‑1 (MQ=255)                                                                                                    ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                     <  2:1058687/140‑1 (MQ=255)                                                                                                      gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  1:562501/136‑1 (MQ=255)                                                                                                      gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       >  2:562501/1‑136 (MQ=255)                                                                                                      gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  2:457112/140‑1 (MQ=255)                                                                                                            gccgccTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTAc                             <  2:299337/140‑1 (MQ=255)                                                                                                             ccgccTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                            >  2:719632/1‑140 (MQ=255)                                                                                                              cgccTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  2:1452562/140‑1 (MQ=255)                                                                                                              cgccTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  1:1590438/140‑1 (MQ=255)                                                                                                              cgccTTCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  2:971222/140‑1 (MQ=255)                                                                                                                   tCTGCGTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATg                      <  1:425173/140‑1 (MQ=255)                                                                                                                        gTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                 <  2:1003899/140‑1 (MQ=255)                                                                                                                        gTCGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                 <  2:220181/140‑1 (MQ=255)                                                                                                                          cGCTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct               <  2:1640142/140‑1 (MQ=255)                                                                                                                            cTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                         >  1:608865/1‑128 (MQ=255)                                                                                                                            cTACAATGGATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                         <  2:608865/128‑1 (MQ=255)                                                                                                                                   ggATTCGAT‑TCCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  2:570773/140‑1 (MQ=255)                                                                                                                                       tcgat‑tcCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       >  1:1302366/1‑135 (MQ=255)                                                                                                                                       tcgat‑tcCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       <  2:1302366/135‑1 (MQ=255)                                                                                                                                       tcgat‑tcCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:1551905/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                       TCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815740‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑