Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT36.4% 121.4 / 61.2 99S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (13/50);  new base T (36/0);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 2.08e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 112 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                          |                                                                                                                                           
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttttt                                                                                                                                           >  2:505017/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGATCAtttttttttt                                                                                                                                          >  2:788519/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttctttt                                                                                                                                         >  1:1366516/1‑140 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttcttttt                                                                                                                                        <  2:90232/140‑1 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttcttttt                                                                                                                                        >  1:1121580/1‑140 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttcttttt                                                                                                                                        <  1:1641004/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTttttctttttct                                                                                                                                      <  1:788519/140‑1 (MQ=255)
      cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCAttttttttttttttt                                                                                                                                     >  1:1130676/1‑137 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGc                                                                                                                                    <  2:1455616/140‑1 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGc                                                                                                                                    <  1:830820/140‑1 (MQ=255)
                aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                           <  1:1548972/140‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATTAGAAGTGAg                                                                                                                       >  2:684776/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTGCCATGAGAAGTGAg                                                                                                                       >  1:1177944/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGAGGGTAACCCTTATAAGCTCCATACCTATACCAGTCGGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGTGAg                                                                                                                       >  1:568528/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGc                                                                                                                                    <  2:1372537/125‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGc                                                                                                                                    >  1:1372537/1‑125 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                     <  2:688916/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                  <  1:409758/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 <  2:1060089/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGCCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCTATTTGAAGTGGGTGCaga                                                                                                                 >  2:409758/1‑140 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                <  1:162525/140‑1 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                     >  2:1552308/1‑132 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                     <  1:1552308/132‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCCGAGAggg                                                                                                            >  2:598576/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCACTGGAAGCGTGCGCAAGGAggg                                                                                                            >  2:275441/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            >  2:891930/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTGCCCTGGGAAGTGAGCGCAGAGAggg                                                                                                            >  2:281738/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 <  2:644203/135‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 >  1:644203/1‑135 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  2:637686/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  2:134704/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            >  1:637686/1‑140 (MQ=255)
                                  ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            >  2:1337720/1‑137 (MQ=255)
                                  ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  1:1337720/137‑1 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                     >  1:568245/1‑140 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                   <  1:324285/140‑1 (MQ=255)
                                            ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCctg                                                                                               >  1:170094/1‑140 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                        <  1:1380607/140‑1 (MQ=255)
                                                   cagcccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                        <  1:1634327/137‑1 (MQ=255)
                                                    aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                                                                       <  2:1121580/140‑1 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGGAGGGGGCGCAGAGAGGGGGTTTTTTGCCTGACTGAACTg                                                                                      >  1:402911/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCCTGACTGAACTg                                                                                      >  2:808502/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGAATGAACtt                                                                                      >  1:781033/1‑139 (MQ=255)
                                                            taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGGGATTa                                                                               >  2:445769/1‑140 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                           <  1:340657/140‑1 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                           <  1:1575447/140‑1 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGAAAGGAGCGCAGAGAGGGGTTTTCTTGCCTGTCTGAACTGGCGAtttaactg                                                                          >  2:1634327/1‑134 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTTTTTCCTGACTGAACTGGCGGTTTAGttt                                                                          >  2:1238623/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                          >  2:232105/1‑140 (MQ=255)
                                                                   cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTCTTCCATGAGGAGGGAGCGCCGGGAGGGGTTTTCTTTCCCGACTGAACTGGCGGTTAAGttttc                                                                        >  2:1379861/1‑138 (MQ=255)
                                                                   cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGttttc                                                                        >  2:1603692/1‑138 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGCCGTCAGTAATGGACGCAGGGAGGGGTTATCTTGCCTGACTGAACTGGGGGTTAAGGTTTCGCG‑TAcc                                                                >  2:852960/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TAcc                                                                <  1:281738/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TAcc                                                                <  1:347599/140‑1 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCCCG‑GAccc                                                               >  2:1404234/1‑140 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCgg                                                             <  1:1603692/140‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCgg                                                             <  1:1379861/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCG‑TACCCcgcgg                                                          >  2:688087/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTTAGAAGTGGGGGCAAAGAGGGGGTATCTTTGCTGACTGAAATTGCGATTTAGATTCTGCG‑CACCCGGCGt                                                          >  2:1505676/1‑140 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCG‑TACCCGGCgg                                                          >  2:3343/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑CACCCCGCGt                                                          >  2:1563931/1‑140 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGt                                                          <  1:114913/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCCCTTTTTTTTTTTCTGTCACCAGGAACGAGGCTGGAGGAGGATTCTCTTGCCCGACCCAACCGGAGCTTAACGTTTCGCG‑GACCCGGCgg                                                          >  2:471672/1‑139 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGtt                                                         >  2:866403/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCATGAGAAGAGAGGGGGGAGAGGGGTTATCTTGGCTGACTGAACTGGCGGTTTAGTTTCCGCG‑GACCCGGCGctt                                                        >  2:43144/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCCGAGGAGAGAGCGCCGGGAGGGGTTTTCTTGCCTGACTGGACTGGCGGTTAAGTTTCCGCGTTACCCgggttt                                                         >  2:1409801/1‑136 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGttt                                                        >  1:1040725/1‑140 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGTGAGAGGAGAGAGGGGTTTTCTTGGCCGTCTGAAATTGCGGTTTAGTTTCCGCG‑TACCCGGCGgttt                                                       >  2:1540705/1‑139 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCG‑TACCCCGCGgttt                                                       >  2:450504/1‑139 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGGGGTGAGCGCCGAGAGGGATTATCTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCG‑TACCCCGCGGTTAGt                                                     >  2:456555/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGAGTTAGTTTTCGCG‑TACCCGGCGTTTTGt                                                     >  2:192275/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAAATGGCGGTTAAGTTTTCGCG‑GACCCGGCGTTTTGt                                                     >  2:307330/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTTCCTGACTGAACTTGCGGTTAAGTTTTCGCG‑TACCCGGCGTTTAGt                                                     >  2:229291/1‑140 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTg                                                <  1:1238623/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTg                                                <  2:1226848/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑g                                             <  1:192275/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑g                                             <  2:779874/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑g                                             <  1:684776/140‑1 (MQ=255)
                                                                                             tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCTTTTTTTTTTTTTTTTTCATGAGAAGTGGGCGCAGGGAGGGGTTATCTTGCCTGACTGAAATGGCGATTAAATTTCCGCG‑GGCCCCGCGGTTTGTTTTTTC‑gg                                            >  2:85631/1‑140 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Gga                                           <  2:794518/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggat                                          <  1:866403/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggatga                                        <  1:1147378/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggatga                                        <  1:927616/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggatga                                        <  1:450504/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggatga                                        <  1:262580/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑Ggatgat                                       <  1:1540705/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTTCTGACTGAACTTGCGGTTTAGGTTTCCCG‑TACCCGGCGTTTTGTTATTTC‑Ggatgatga                                     >  2:479071/1‑140 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGG‑CCATTTTTTCTTTTTTTGCCATTAGAAGAGAGCGCCGCGAGGGATTATCTTGTCTGACTTAACCGGCGATTAAATTTCCGCG‑CATCCGCCGGTTTGTTATTGC‑Cgatgatgg                                     >  2:1014481/1‑139 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCt                           <  2:1340211/140‑1 (MQ=255)
                                                                                                               gATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGTCCTGAGAAGGGAGCGGAGGGAAGGATGTTCTTTCCTGGCTGAACTGGGGGTTAAAATTTCGCG‑CACCCCGCGATTGGTTATTGG‑GGGTGGTGGTATTTAAGccc                          >  2:1283311/1‑138 (MQ=255)
                                                                                                               gATCTCGTTTGCGGCGG‑TCAATTTTTTTTTTTTTGCCATGAGAAATGAACGGAGCGAGGGATTATCTTTCCTCACTGGACTGGGGATTAAGTTTTCACG‑CACCCGGCGGTTAGTTTTTTCGGGATCA‑GATATTGAAGcac                          >  2:1123345/1‑138 (MQ=255)
                                                                                                                 tctcGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCgg                        <  2:1366516/140‑1 (MQ=255)
                                                                                                                     gTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGa                    <  1:505017/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGTGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTGTTGC‑GGATGATGATATTGAAGCTCGGCTGAt                   <  1:471672/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGAt                   <  1:1014481/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGAt                   <  2:1130676/140‑1 (MQ=255)
                                                                                                                          cggcgg‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGATTGGt               <  1:79059/140‑1 (MQ=255)
                                                                                                                            gcgg‑TCTTTTTTTCTTTTTCTGCCATGGGAAGTGAGCGCAGAGAGGGGCTTTTTTGCCTTGGTGTACTTGCGGGTTAGTTTCCTCC‑GGCCCCCGGTTTTTTTATTGC‑GGCTGGTGATATTGAAGCTCGGCTGATTGGttt             >  2:792375/1‑139 (MQ=255)
                                                                                                                                       tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGC‑CGGCTGATTGGTTCATTGTCGCCAt  <  2:1498249/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:229291/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCG‑TACCCGGCGTTTAGTTATTGC‑GGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑