BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,199	G→T	A270S (GCC→TCC)	srlE →	sorbitol‑specific PTS enzyme IIBC1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,199	0	G	T	100.0%	410.2 / NA	117	A270S (GCC→TCC)	srlE	sorbitol‑specific PTS enzyme IIBC1 component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (64/53); total (64/53)

Only 100 of 117 total aligned reads displayed.

CACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTC  >  NC_000913/2827062‑2827332

  cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTg                                                                                                                                   <  1:584725/140‑1 (MQ=255)

       ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACt                                                                                                                              <  1:769032/140‑1 (MQ=255)

           ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATCGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCAt                                                                                                                          <  2:1204763/140‑1 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTg                                                                                                                                   <  1:1608510/127‑1 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTg                                                                                                                                   >  2:1608510/1‑127 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGc                                                                                                                                  <  2:990656/128‑1 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGc                                                                                                                                  >  1:990656/1‑128 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCcg                                                                                                                      >  2:932397/1‑140 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCcg                                                                                                                      >  1:1213756/1‑140 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCcg                                                                                                                      <  2:1213756/140‑1 (MQ=255)

               tCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCcg                                                                                                                      >  2:1385331/1‑140 (MQ=255)

                    ggCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCcggtcgg                                                                                                                 >  1:522648/1‑140 (MQ=255)

                      cccAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGtt                                                                                                               >  1:1374334/1‑140 (MQ=255)

                      cccAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGtt                                                                                                               >  1:1273888/1‑140 (MQ=255)

                      cccAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGtt                                                                                                               >  2:393060/1‑140 (MQ=255)

                           gcgcAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCg                                                                                                          >  2:208160/1‑140 (MQ=255)

                                gTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGc                                                                                                     >  2:1341106/1‑140 (MQ=255)

                                gTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGc                                                                                                     >  2:1111106/1‑140 (MQ=255)

                                gTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGc                                                                                                     <  1:208160/140‑1 (MQ=255)

                                 ttATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCg                                                                                                    <  1:1085997/140‑1 (MQ=255)

                                     cGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAg                                                                                                <  2:1282199/140‑1 (MQ=255)

                                         caGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCg                                                                                            >  1:1234993/1‑140 (MQ=255)

                                           ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTc                                                                                          >  2:1581503/1‑140 (MQ=255)

                                           ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTc                                                                                          >  2:135552/1‑140 (MQ=255)

                                           ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTc                                                                                          >  2:1442747/1‑140 (MQ=255)

                                           ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTc                                                                                          >  2:822828/1‑140 (MQ=255)

                                             ttATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAg                                                                                        >  1:1578681/1‑140 (MQ=255)

                                              tATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAgg                                                                                       <  1:1581503/140‑1 (MQ=255)

                                               aTCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAg                                                                                                >  2:1048865/1‑130 (MQ=255)

                                               aTCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAg                                                                                                <  1:1048865/130‑1 (MQ=255)

                                                tCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGac                                                                                     >  1:1338965/1‑140 (MQ=255)

                                                 cGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGaca                                                                                    <  2:1159109/140‑1 (MQ=255)

                                                   gCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACg                                                                                  <  2:1500612/140‑1 (MQ=255)

                                                    cGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACgg                                                                                 >  2:606057/1‑140 (MQ=255)

                                                     gTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGt                                                                                >  2:113442/1‑140 (MQ=255)

                                                      tATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGtt                                                                               >  1:1518705/1‑140 (MQ=255)

                                                      tATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGtt                                                                               >  2:270310/1‑140 (MQ=255)

                                                      tATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGtt                                                                               >  2:1437691/1‑140 (MQ=255)

                                                       attgattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTc                                                                              >  2:1492915/1‑140 (MQ=255)

                                                       attgattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTc                                                                              >  2:1054639/1‑140 (MQ=255)

                                                        ttgattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTcg                                                                             <  1:1385331/140‑1 (MQ=255)

                                                          gattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTcgcg                                                                           <  1:135552/140‑1 (MQ=255)

                                                           attgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGt                                                                          <  2:491599/140‑1 (MQ=255)

                                                            ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTc                                                                         <  2:1273888/140‑1 (MQ=255)

                                                             tgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCg                                                                        >  2:226905/1‑140 (MQ=255)

                                                                cGTGCAGATTGGTCTCGGCAATATTCCTCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGCCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtg                                                                     >  2:1668163/1‑140 (MQ=255)

                                                                cGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtg                                                                     >  1:1260165/1‑140 (MQ=255)

                                                                cGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtg                                                                     >  1:623044/1‑140 (MQ=255)

                                                                 gTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtgt                                                                    <  1:1442747/140‑1 (MQ=255)

                                                                 gTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtgt                                                                    <  1:1281041/140‑1 (MQ=255)

                                                                 gTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtgt                                                                    <  1:270310/140‑1 (MQ=255)

                                                                    cAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTccc                                                                 <  2:1627721/140‑1 (MQ=255)

                                                                    cAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTccc                                                                 <  2:522648/140‑1 (MQ=255)

                                                                     aGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCt                                                                >  1:940260/1‑140 (MQ=255)

                                                                     aGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCt                                                                >  2:1659890/1‑140 (MQ=255)

                                                                     aGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCt                                                                >  2:765279/1‑140 (MQ=255)

                                                                      gATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCtt                                                               <  1:1659890/140‑1 (MQ=255)

                                                                      gATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCtt                                                               <  1:1492915/140‑1 (MQ=255)

                                                                      gATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCtt                                                               <  1:1054639/140‑1 (MQ=255)

                                                                       aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTc                                                              >  2:1171319/1‑140 (MQ=255)

                                                                       aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTACATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTc                                                              <  1:1014076/140‑1 (MQ=255)

                                                                         tGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTg                                                            >  1:779737/1‑140 (MQ=255)

                                                                              tcGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTg                                                       >  2:738981/1‑140 (MQ=255)

                                                                                 gCAATATTCCGCCGCATCTGGCTTTACCGGCGCTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTg                                                    >  1:604832/1‑140 (MQ=255)

                                                                                 gCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTg                                                    >  1:379272/1‑140 (MQ=255)

                                                                                          cgccgcATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCttt                                           >  1:824405/1‑140 (MQ=255)

                                                                                            ccgcATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGCCCCTTCTGTACTGGTGAGCCGCttttt                                         >  2:1172033/1‑140 (MQ=255)

                                                                                               cATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGc                                                 <  1:715835/129‑1 (MQ=255)

                                                                                               cATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGc                                                 >  2:715835/1‑129 (MQ=255)

                                                                                               cATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAAc                                      >  1:464734/1‑140 (MQ=255)

                                                                                               cATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAAc                                      >  2:1058993/1‑140 (MQ=255)

                                                                                               cATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGAGAGCCGCTTTTTAAc                                      >  1:730699/1‑140 (MQ=255)

                                                                                                aTCTGGCTTTACCGGCGCTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAAcc                                     <  2:604832/140‑1 (MQ=255)

                                                                                                  cTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCgg                                   <  2:824405/140‑1 (MQ=255)

                                                                                                     gCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgc                                <  1:1111106/140‑1 (MQ=255)

                                                                                                     gCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGCCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgc                                <  1:1172033/140‑1 (MQ=255)

                                                                                                      cTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgcg                               <  1:113442/140‑1 (MQ=255)

                                                                                                       tttACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgcgc                              <  2:779737/140‑1 (MQ=255)

                                                                                                        ttACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTTGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGcc                             <  2:188740/140‑1 (MQ=255)

                                                                                                        ttACCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGcc                             <  2:1338965/140‑1 (MQ=255)

                                                                                                          aCCGGCACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGa                           <  1:202977/140‑1 (MQ=255)

                                                                                                               cACTGTTTGCCATCAACGCGCAGGCGTCGTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTa                      >  2:1539428/1‑140 (MQ=255)

                                                                                                               cACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCATGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGGCTGTa                      >  2:6924/1‑140 (MQ=255)

                                                                                                               cACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTa                      >  2:1328485/1‑140 (MQ=255)

                                                                                                               cACTGTTTGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTa                      >  1:930191/1‑140 (MQ=255)

                                                                                                                    tttGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgc                                <  1:337132/125‑1 (MQ=255)

                                                                                                                    tttGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgc                                >  2:337132/1‑125 (MQ=255)

                                                                                                                    tttGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGAt                 >  1:693474/1‑140 (MQ=255)

                                                                                                                    tttGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGAt                 >  2:320514/1‑140 (MQ=255)

                                                                                                                    tttGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTCGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGAt                 >  1:797050/1‑140 (MQ=255)

                                                                                                                      tGCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCg               <  1:822828/140‑1 (MQ=255)

                                                                                                                       gCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGa                           >  2:1405310/1‑127 (MQ=255)

                                                                                                                       gCCATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGa                           <  1:1405310/127‑1 (MQ=255)

                                                                                                                        ccATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGcc             <  2:730699/140‑1 (MQ=255)

                                                                                                                        ccATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGcc             <  1:226905/140‑1 (MQ=255)

                                                                                                                        ccATCAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGcc             <  2:1234993/140‑1 (MQ=255)

                                                                                                                            cAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGt         <  2:977126/140‑1 (MQ=255)

                                                                                                                            cAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGt         >  1:411500/1‑140 (MQ=255)

                                                                                                                            cAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGt         >  1:936689/1‑140 (MQ=255)

                                                                                                                            cAACGCGCAGGCGTCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGt         >  2:513520/1‑140 (MQ=255)

CACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTC  >  NC_000913/2827062‑2827332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`