Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	639,419	G→T	A159S (GCT→TCT)	ahpC →	alkyl hydroperoxide reductase, AhpC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	639,419	0	G	T	100.0%	228.3 / NA	65	A159S (GCT→TCT)	ahpC	alkyl hydroperoxide reductase, AhpC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/34);  total (31/34)

TGAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCATAGCGGCGTTGGCGTCGCCCGCTC  >  NC_000913/639286‑639554                                                                                                                                      |                                                                                                                                        tGAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTtctc                                                                                                                                   >  2:652439/1‑140 (MQ=255)  gAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCa                                                                                                                                  >  1:1430274/1‑140 (MQ=255)        cTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAgg                                                                                                                            <  1:1181515/140‑1 (MQ=255)         tGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGt                                                                                                                           >  2:121054/1‑140 (MQ=255)         tGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGt                                                                                                                           >  1:216680/1‑140 (MQ=255)          ggCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTg                                                                                                                          >  1:1261772/1‑140 (MQ=255)          ggCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTg                                                                                                                          >  2:1465696/1‑140 (MQ=255)                ccGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCAc                                                                                                                                 <  1:1057918/127‑1 (MQ=255)                ccGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCAc                                                                                                                                 >  2:1057918/1‑127 (MQ=255)                ccGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGttt                                                                                                                    >  1:218436/1‑140 (MQ=255)                 cGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTg                                                                                                                   >  1:588341/1‑140 (MQ=255)                   tGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGcc                                                                                                                 <  2:1418341/140‑1 (MQ=255)                   tGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGcc                                                                                                                 <  2:369062/140‑1 (MQ=255)                   tGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGcc                                                                                                                 >  1:425502/1‑140 (MQ=255)                   tGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGcc                                                                                                                 <  2:758216/140‑1 (MQ=255)                     cGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCg                                                                                                               >  1:778142/1‑140 (MQ=255)                       aCCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCCTCTCACCCAGGTGAAGTTTGCCCGGc                                                                                                             <  2:1342653/140‑1 (MQ=255)                        ccTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCt                                                                                                            <  1:1492347/140‑1 (MQ=255)                           tCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTaaa                                                                                                         <  1:175345/140‑1 (MQ=255)                                 ttgACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGaaa                                                                                                   <  1:552505/140‑1 (MQ=255)                                  tgACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAaag                                                                                                  <  2:1261772/140‑1 (MQ=255)                                    aCCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAaagaa                                                                                                >  1:994110/1‑140 (MQ=255)                                     cccGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAaagaag                                                                                               >  1:889557/1‑140 (MQ=255)                                      ccGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAgg                                                                                              >  1:240160/1‑140 (MQ=255)                                      ccGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAgg                                                                                              <  2:778142/140‑1 (MQ=255)                                         cAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGa                                                                                           >  1:955454/1‑140 (MQ=255)                                         cAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGCAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGa                                                                                           >  1:1492467/1‑140 (MQ=255)                                          aGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGaa                                                                                          <  2:588341/140‑1 (MQ=255)                                           gggTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAg                                                                                         <  2:425502/140‑1 (MQ=255)                                           gggTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAg                                                                                         <  1:652439/140‑1 (MQ=255)                                              tATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCaa                                                                                      <  1:121054/140‑1 (MQ=255)                                               atcatcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAAc                                                                                     >  1:28135/1‑140 (MQ=255)                                                tcatcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAAct                                                                                    <  2:332416/140‑1 (MQ=255)                                                   tcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGc                                                                                        <  1:826682/133‑1 (MQ=255)                                                   tcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGc                                                                                        <  1:426009/133‑1 (MQ=255)                                                   tcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGc                                                                                        >  2:426009/1‑133 (MQ=255)                                                   tcCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGc                                                                                        >  2:826682/1‑133 (MQ=255)                                                      aGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCt                                                                              >  1:1039131/1‑140 (MQ=255)                                                       ggCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTc                                                                             <  2:1039131/140‑1 (MQ=255)                                                       ggCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTc                                                                             >  2:666458/1‑140 (MQ=255)                                                       ggCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTc                                                                             <  2:928601/140‑1 (MQ=255)                                                       ggCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGCAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTc                                                                             <  2:1492467/140‑1 (MQ=255)                                                        gCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTcc                                                                            <  1:666458/140‑1 (MQ=255)                                                        gCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTcc                                                                            <  1:1465696/140‑1 (MQ=255)                                                        gCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTcc                                                                            <  1:1209366/140‑1 (MQ=255)                                                        gCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTcc                                                                            >  1:1614479/1‑140 (MQ=255)                                                            tCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGTCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGtct                                                                        >  1:4657/1‑140 (MQ=255)                                                             cGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGtctc                                                                       <  2:955454/140‑1 (MQ=255)                                                                  ttACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGAc                                                                  <  2:889557/140‑1 (MQ=255)                                                                  ttACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGAc                                                                  <  2:240160/140‑1 (MQ=255)                                                                  ttACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGAc                                                                  <  2:1614479/140‑1 (MQ=255)                                                                      cGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTgg                                                                                >  1:514054/1‑122 (MQ=255)                                                                      cGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTgg                                                                                <  2:514054/122‑1 (MQ=255)                                                                            aGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGTCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTa                                                        <  2:4657/140‑1 (MQ=255)                                                                            aGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTa                                                        >  1:1586752/1‑140 (MQ=255)                                                                                aTTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTaaaa                                                     >  2:336789/1‑139 (MQ=255)                                                                                aTTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTaaaa                                                     <  1:336789/139‑1 (MQ=255)                                                                                        tGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAAttt                                            >  2:1004110/1‑140 (MQ=255)                                                                                           cgcgTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCt                                         <  2:28135/140‑1 (MQ=255)                                                                                              gTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCg                                      >  2:523805/1‑140 (MQ=255)                                                                                                       gctgcGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACg                             <  1:1004110/140‑1 (MQ=255)                                                                                                          gcGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCa                          >  1:560824/1‑140 (MQ=255)                                                                                                          gcGTAAAATCAAAGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCa                          >  2:1459070/1‑140 (MQ=255)                                                                                                                     aaGCAGCACAGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCATAGCGGCGTTg               >  1:147274/1‑140 (MQ=255)                                                                                                                           cacaGTACGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCATAGCGGCGTTGGCGTCg         >  2:763184/1‑140 (MQ=255)                                                                                                                                  cGTATCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCATAGCGGCGTTGGCGTCGCCCGCTc  <  2:994110/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGAAGGTCTGGCTGACCGTGCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGCATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCTCACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCTCTGGACCTGGTTGGTAAAATCTAAATTTCCTTCGTCTTTCACGCCATAGCGGCGTTGGCGTCGCCCGCTC  >  NC_000913/639286‑639554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑