Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,017	G→T	V133V (GTG→GTT)	lolD →	lipoprotein release complex ‑ ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,017	0	G	T	100.0%	179.6 / NA	52	V133V (GTG→GTT)	lolD	lipoprotein release complex ‑ ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/22);  total (30/22)

TGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGC  >  NC_000913/1176884‑1177153                                                                                                                                      |                                                                                                                                         tGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTg                                                                                                                                    >  1:663636/1‑140 (MQ=255)   ggCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGa                                                                                                                                  >  1:475634/1‑140 (MQ=255)   ggCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGa                                                                                                                                  >  1:1386868/1‑140 (MQ=255)           ttatCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTg                                                                                                                          <  2:475634/140‑1 (MQ=255)             atCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCg                                                                                                                        >  1:206380/1‑140 (MQ=255)                aGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAAt                                                                                                                     >  2:1366475/1‑140 (MQ=255)                  tttCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCa                                                                                                                   >  1:1031672/1‑140 (MQ=255)                     caccacCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCg                                                                                                                >  2:1371034/1‑140 (MQ=255)                     caccacCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCg                                                                                                                <  1:533049/140‑1 (MQ=255)                     caccacCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCg                                                                                                                <  1:1156021/140‑1 (MQ=255)                     caccacCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCg                                                                                                                >  2:242996/1‑140 (MQ=255)                         accTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCa                                                                                                            <  2:663636/140‑1 (MQ=255)                            tgctgcCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCGCTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCt                                                                                                         >  2:1279/1‑140 (MQ=255)                            tgctgcCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCt                                                                                                         >  2:873810/1‑140 (MQ=255)                             gctgcCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTg                                                                                                        >  2:1416060/1‑140 (MQ=255)                             gctgcCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTg                                                                                                        >  2:1196809/1‑140 (MQ=255)                                gcCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAAc                                                                                                     >  1:696300/1‑140 (MQ=255)                                  cGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACtt                                                                                                   >  1:266510/1‑140 (MQ=255)                                      ttttACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTg                                                                                               <  1:873810/140‑1 (MQ=255)                                         tACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTggcg                                                                                            >  1:1308415/1‑140 (MQ=255)                                           ccGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTggcggc                                                                                          <  2:1386868/140‑1 (MQ=255)                                               ccTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAAc                                                                                      >  2:9419/1‑140 (MQ=255)                                                          tGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg                                                                             <  1:264292/138‑1 (MQ=255)                                                          tGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg                                                                             >  2:264292/1‑138 (MQ=255)                                                             cTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCg                                                                        >  2:157344/1‑140 (MQ=255)                                                                  ccGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGc                                                                   >  2:1107843/1‑140 (MQ=255)                                                                    gctgctGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGccc                                                                 >  1:184959/1‑140 (MQ=255)                                                                      tgctgATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGt                                                               >  1:812010/1‑140 (MQ=255)                                                                       gctgATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCTTCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTg                                                              <  2:1608538/140‑1 (MQ=255)                                                                       gctgATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGAt                                                                      >  1:294683/1‑132 (MQ=255)                                                                       gctgATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGAt                                                                      <  2:294683/132‑1 (MQ=255)                                                                         tgATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTgcg                                                            <  1:1416060/140‑1 (MQ=255)                                                                           aTTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCt                                                          <  2:456583/140‑1 (MQ=255)                                                                           aTTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCt                                                          >  2:608369/1‑140 (MQ=255)                                                                           aTTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCt                                                          <  1:9419/140‑1 (MQ=255)                                                                           aTTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCt                                                          <  1:1366475/140‑1 (MQ=255)                                                                                      aaaGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAcc                                               <  1:1196809/140‑1 (MQ=255)                                                                                        aGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcg                                             <  1:868009/140‑1 (MQ=255)                                                                                         gCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc                                            >  1:1365380/1‑140 (MQ=255)                                                                                            cGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGcc                                         <  2:206380/140‑1 (MQ=255)                                                                                              cTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTg                                       >  1:1035185/1‑140 (MQ=255)                                                                                                   aTCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACt                                  <  2:658435/140‑1 (MQ=255)                                                                                                          gCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGAt                           <  1:1371034/140‑1 (MQ=255)                                                                                                          gCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGAt                           <  2:812010/140‑1 (MQ=255)                                                                                                          gCCGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGAt                           <  1:242996/140‑1 (MQ=255)                                                                                                           ccGTGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATg                          <  1:1107843/140‑1 (MQ=255)                                                                                                              tGCACTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAAc                       >  1:1535423/1‑140 (MQ=255)                                                                                                                 aCTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTa                    >  2:1221172/1‑140 (MQ=255)                                                                                                                 aCTTGAGATGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTa                    <  2:1184902/140‑1 (MQ=255)                                                                                                                     gagaTGTTAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCgg                >  2:273860/1‑140 (MQ=255)                                                                                                                           ttAAAAGCGGTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCt          >  1:711467/1‑140 (MQ=255)                                                                                                                                   ggTTGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAGCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATgcgc  >  2:748441/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         TGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGC  >  NC_000913/1176884‑1177153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑