Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	430,451	C→A	A316E (GCG→GAG)	secF →	Sec translocon accessory complex subunit SecF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	430,451	0	C	A	100.0%	337.8 / NA	95	A316E (GCG→GAG)	secF	Sec translocon accessory complex subunit SecF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (50/45);  total (50/45)

GAAGGCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAT  >  NC_000913/430315‑430585                                                                                                                                         |                                                                                                                                       gAAGGCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGggagga                                                                                                                                     >  1:471187/1‑140 (MQ=255) gAAGGCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGggagga                                                                                                                                     >  1:380470/1‑140 (MQ=255)  aaGGCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGAt                                                                                                                                    <  2:1620045/140‑1 (MQ=255)    ggCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCa                                                                                                                                  <  2:938189/140‑1 (MQ=255)    ggCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCa                                                                                                                                  <  2:1248895/140‑1 (MQ=255)         ctcGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGt                                                                                                                             >  1:792766/1‑140 (MQ=255)         ctcGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGt                                                                                                                             <  2:1385637/140‑1 (MQ=255)              tGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAAtt                                                                                                                        <  2:471187/140‑1 (MQ=255)              tGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAAtt                                                                                                                        >  1:344005/1‑140 (MQ=255)                 ccATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTg                                                                                                                     <  2:380470/140‑1 (MQ=255)                   aTGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCa                                                                                                                                  <  1:125789/125‑1 (MQ=255)                   aTGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCa                                                                                                                                  >  2:125789/1‑125 (MQ=255)                     gCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCg                                                                                                                  >  2:1577006/1‑139 (MQ=255)                     gCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCg                                                                                                                  <  1:1577006/139‑1 (MQ=255)                          tCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCa                                                                                                            >  2:401146/1‑140 (MQ=255)                            ggTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAg                                                                                                          >  2:1224342/1‑140 (MQ=255)                                tttCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTcc                                                                                                      >  2:8899/1‑140 (MQ=255)                                     aTCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTg                                                                                                 <  2:344005/140‑1 (MQ=255)                                          tACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGtt                                                                                            >  2:352926/1‑140 (MQ=255)                                               cATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGGGCGAACACATGTTGCAGCAGAAATTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGaaaa                                                                                       >  1:636450/1‑140 (MQ=255)                                                 tCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATc                                                                                     <  2:313290/140‑1 (MQ=255)                                                 tCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATc                                                                                     <  1:1224342/140‑1 (MQ=255)                                                 tCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATc                                                                                     <  2:1532473/140‑1 (MQ=255)                                                   ttCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATccc                                                                                   <  2:792766/140‑1 (MQ=255)                                                   ttCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATccc                                                                                   >  2:1085962/1‑140 (MQ=255)                                                    tCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGAt                                                                                               >  1:160916/1‑127 (MQ=255)                                                    tCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGAt                                                                                               <  2:160916/127‑1 (MQ=255)                                                    tCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATccc                                                                                   >  2:911853/1‑139 (MQ=255)                                                    tCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATccc                                                                                   <  1:911853/139‑1 (MQ=255)                                                    tCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTG‑AAATCCCgg                                                                                 >  1:1414994/1‑140 (MQ=255)                                                        tCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCa                                                                              >  2:374436/1‑140 (MQ=255)                                                         cTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCag                                                                             >  1:715448/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  2:108269/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  1:862462/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  1:566927/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  1:216972/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  2:451909/1‑140 (MQ=255)                                                            tgtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaag                                                                          >  2:876016/1‑140 (MQ=255)                                                             gtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaaga                                                                         <  1:1116869/140‑1 (MQ=255)                                                              tgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGAt                                                                        >  1:702154/1‑140 (MQ=255)                                                              tgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGAt                                                                        >  1:466790/1‑140 (MQ=255)                                                              tgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGAt                                                                        <  1:401146/140‑1 (MQ=255)                                                              tgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGAt                                                                        >  1:315590/1‑140 (MQ=255)                                                                  aTCTGCGTTGGCTCTGAAACT‑GGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGa                                                                   >  1:682999/1‑140 (MQ=255)                                                                   tCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGa                                                                   >  2:1128979/1‑140 (MQ=255)                                                                      gCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttt                                                                >  2:1130566/1‑140 (MQ=255)                                                                      gCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAt                                                                  >  1:1503194/1‑138 (MQ=255)                                                                      gCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAt                                                                  <  2:1503194/138‑1 (MQ=255)                                                                       cGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttt                                                               >  2:482168/1‑140 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  1:352926/140‑1 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  1:482168/140‑1 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  2:715448/140‑1 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  2:702154/140‑1 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  2:466790/140‑1 (MQ=255)                                                                         ttGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                             <  2:1046736/140‑1 (MQ=255)                                                                           ggCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttttt                                                           <  2:970429/140‑1 (MQ=255)                                                                            gCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTg                                                          >  2:984283/1‑140 (MQ=255)                                                                            gCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTg                                                          <  2:216972/140‑1 (MQ=255)                                                                             ctctGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGa                                                         <  2:315590/140‑1 (MQ=255)                                                                                 gAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTa                                                     >  1:528057/1‑140 (MQ=255)                                                                                 gAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTa                                                     <  1:1130566/140‑1 (MQ=255)                                                                                   aaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATg                                                   <  1:374436/140‑1 (MQ=255)                                                                                      tGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGgag                                                >  2:938595/1‑140 (MQ=255)                                                                                      tGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGgag                                                >  2:1629278/1‑140 (MQ=255)                                                                                            tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCg                                          >  2:267439/1‑140 (MQ=255)                                                                                               aGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGaaa                                       <  1:1629278/140‑1 (MQ=255)                                                                                               aGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGaaa                                       <  1:108269/140‑1 (MQ=255)                                                                                                  gcgAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATc                                    >  1:1142365/1‑140 (MQ=255)                                                                                                   cgAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATgg                                                  <  2:139937/125‑1 (MQ=255)                                                                                                   cgAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATgg                                                  >  1:139937/1‑125 (MQ=255)                                                                                                   cgAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATcc                                   >  1:1280930/1‑140 (MQ=255)                                                                                                    gAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATccc                                  <  2:948866/140‑1 (MQ=255)                                                                                                        acaTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCa                              <  1:451909/140‑1 (MQ=255)                                                                                                        acaTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCa                              <  2:31627/140‑1 (MQ=255)                                                                                                              tGCAGCAGAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                        >  1:434784/1‑140 (MQ=255)                                                                                                                cagcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGa                      >  2:1178015/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  1:890674/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  2:217309/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    <  1:876016/140‑1 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  1:790599/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  2:574541/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  2:570267/1‑140 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    <  2:566927/140‑1 (MQ=255)                                                                                                                  gcagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  1:1367963/1‑140 (MQ=255)                                                                                                                   cagAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAAc                   >  2:670758/1‑140 (MQ=255)                                                                                                                    agAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACt                  >  1:729840/1‑140 (MQ=255)                                                                                                                    agAAAGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACt                  >  1:1442842/1‑140 (MQ=255)                                                                                                                       aaGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAACCCCCGCATCTTGGGAAACTgcg               <  2:636450/140‑1 (MQ=255)                                                                                                                        aGTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgcgc              >  1:1232801/1‑140 (MQ=255)                                                                                                                         gTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGcgcg             <  2:528057/140‑1 (MQ=255)                                                                                                                         gTGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATATATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGcgcg             <  1:1128979/140‑1 (MQ=255)                                                                                                                          tGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGt            <  2:1367963/140‑1 (MQ=255)                                                                                                                          tGGAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTG‑AAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTa           <  2:1414994/140‑1 (MQ=255)                                                                                                                           ggAAAAAGAAGGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTa           <  1:267439/140‑1 (MQ=255)                                                                                                                                aagaagGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    <  1:1336578/126‑1 (MQ=255)                                                                                                                                aagaagGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGaaa                    >  2:1336578/1‑126 (MQ=255)                                                                                                                                aagaagGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCt      <  1:570267/140‑1 (MQ=255)                                                                                                                                    agGGGAGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAt  <  2:869023/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GAAGGCTTCTCGCTGACCATGCTTATCGGTGTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAT  >  NC_000913/430315‑430585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑