Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,506,124	C→A	intergenic (‑44/‑48)	ydcO ← / → sutR	putative transport protein YdcO/DNA‑binding transcriptional dual regulator SutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,506,124	0	C	A	100.0%	134.9 / NA	39	intergenic (‑44/‑48)	ydcO/sutR	putative transport protein YdcO/DNA‑binding transcriptional dual regulator SutR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/14);  total (25/14)

GCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGCG  >  NC_000913/1505990‑1506258                                                                                                                                       |                                                                                                                                       gcaaGATTATGGCCGCTGAACGGGGGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTta                                                                                                                                   <  1:52720/137‑1 (MQ=255)                                       cccGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGgaggag                                                                                             <  1:946547/139‑1 (MQ=255)                                       aCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGgaggag                                                                                             >  2:886218/1‑140 (MQ=255)                                                          gTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgct                                                                          <  1:1015178/140‑1 (MQ=255)                                                          gTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgct                                                                          <  2:860103/140‑1 (MQ=255)                                                          gTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgct                                                                          <  1:995443/140‑1 (MQ=255)                                                           tAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgctc                                                                         >  2:502762/1‑140 (MQ=255)                                                                tgggtggAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCttt                                                                    >  1:617886/1‑140 (MQ=255)                                                                  ggtggAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCttttt                                                                  >  1:837693/1‑140 (MQ=255)                                                                         gaATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgc                                                                           <  2:514508/124‑1 (MQ=255)                                                                         gaATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATctcgc                                                                           >  1:514508/1‑124 (MQ=255)                                                                         gaATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATccacc                                                           >  2:968414/1‑140 (MQ=255)                                                                         gaATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATccacc                                                           <  2:1545588/140‑1 (MQ=255)                                                                                 aaCAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTaa                                                   <  1:1394100/140‑1 (MQ=255)                                                                                    aGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAaaca                                                <  1:293374/140‑1 (MQ=255)                                                                                                aCCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCcagca                                    >  1:85204/1‑140 (MQ=255)                                                                                                 ccTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCcagcag                                   <  2:617886/140‑1 (MQ=255)                                                                                                   tCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAgcg                                 <  2:837693/140‑1 (MQ=255)                                                                                                    cGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAgcgc                                <  2:1151777/140‑1 (MQ=255)                                                                                                      tGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCgg                              >  2:571139/1‑140 (MQ=255)                                                                                                      tGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCgg                              >  1:153885/1‑140 (MQ=255)                                                                                                      tGCGTTATAGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCgg                              >  1:891924/1‑140 (MQ=255)                                                                                                            ataGCGTACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAg                        >  2:623076/1‑140 (MQ=255)                                                                                                                  tACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTc                  >  1:921766/1‑140 (MQ=255)                                                                                                                  tACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTc                  >  2:632053/1‑140 (MQ=255)                                                                                                                  tACAGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCACTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTc                  >  2:419636/1‑140 (MQ=255)                                                                                                                     aGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTcgcg               >  2:643762/1‑140 (MQ=255)                                                                                                                     aGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTcgcg               >  1:166503/1‑140 (MQ=255)                                                                                                                     aGGCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTcgcg               >  1:816220/1‑140 (MQ=255)                                                                                                                      ggCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGa              <  1:643762/140‑1 (MQ=255)                                                                                                                       gCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGAc             >  1:1617203/1‑140 (MQ=255)                                                                                                                       gCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGAc             >  2:166210/1‑140 (MQ=255)                                                                                                                       gCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGAc             >  1:1612900/1‑140 (MQ=255)                                                                                                                       gCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGAc             >  2:1045940/1‑140 (MQ=255)                                                                                                                       gCACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGAc             >  1:574486/1‑140 (MQ=255)                                                                                                                        cACCGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACt            <  1:166210/140‑1 (MQ=255)                                                                                                                          ccGTAGCATTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTgg          >  2:1344763/1‑140 (MQ=255)                                                                                                                                 aTTTGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGc   >  2:1403688/1‑140 (MQ=255)                                                                                                                                  tttGTACGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGCg  >  2:275033/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAAAATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGCG  >  NC_000913/1505990‑1506258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑