Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,815,467	C→A	E325* (GAA→TAA)	gshA ←	glutamate‑‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,815,467	0	C	A	100.0%	266.8 / NA	75	E325* (GAA→TAA)	gshA	glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/34);  total (41/34)

TTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCT  >  NC_000913/2815333‑2815601                                                                                                                                       |                                                                                                                                       ttCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATgc                                                                                                                                   <  2:327340/140‑1 (MQ=255) ttCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATgc                                                                                                                                   <  1:953799/140‑1 (MQ=255) ttCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATgc                                                                                                                                   >  2:1092944/1‑140 (MQ=255)   cGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGACCGCACTTCAATATATTAAATgccg                                                                                                                                 <  2:1026548/140‑1 (MQ=255)   cGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATgccg                                                                                                                                 <  2:358651/140‑1 (MQ=255)   cGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATgccg                                                                                                                                 <  2:218042/140‑1 (MQ=255)                ttCCGGTGCATCAGCCAGCGCACCCCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACTCCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAgcg                                                                                                                    <  2:577898/140‑1 (MQ=255)                ttCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAgcg                                                                                                                    <  2:563623/140‑1 (MQ=255)                   cGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCAt                                                                                                                 <  1:1161259/140‑1 (MQ=255)                             gccagcGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGa                                                                                                       <  2:1142243/140‑1 (MQ=255)                                agcGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAgcg                                                                                                                    <  2:200497/124‑1 (MQ=255)                                agcGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAgcg                                                                                                                    >  1:200497/1‑124 (MQ=255)                                            aCCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGt                                                                                        >  2:446303/1‑140 (MQ=255)                                                 aaaCAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTc                                                                                                    <  1:1365169/123‑1 (MQ=255)                                                 aaaCAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTc                                                                                                    >  2:1365169/1‑123 (MQ=255)                                                 aaaCAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAAcgc                                                                                   >  1:190978/1‑140 (MQ=255)                                                 aaaCAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAAcgc                                                                                   >  1:881360/1‑140 (MQ=255)                                                       gTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                             >  1:348327/1‑140 (MQ=255)                                                       gTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                             >  2:1136802/1‑140 (MQ=255)                                                       gTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                             >  2:515492/1‑140 (MQ=255)                                                       gTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                             >  2:372240/1‑140 (MQ=255)                                                        tCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGGGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTgg                                                                            <  2:382557/140‑1 (MQ=255)                                                        tCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTgg                                                                            >  2:226846/1‑140 (MQ=255)                                                        tCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTgg                                                                            >  2:899200/1‑140 (MQ=255)                                                        tCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTgg                                                                            >  2:679763/1‑140 (MQ=255)                                                         cGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGa                                                                           >  2:708577/1‑140 (MQ=255)                                                                tCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCg                                                                    >  2:1014726/1‑140 (MQ=255)                                                                 cGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCgg                                                                   >  1:834377/1‑140 (MQ=255)                                                                  gCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGgc                                                                  <  1:1014726/140‑1 (MQ=255)                                                                  gCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGgc                                                                  >  1:56307/1‑140 (MQ=255)                                                                     ccTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGcgcg                                                               <  2:138839/140‑1 (MQ=255)                                                                         ctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACa                                                           >  1:352017/1‑140 (MQ=255)                                                                                tCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGtt                                                    >  2:1051024/1‑140 (MQ=255)                                                                                   acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTc                                                 >  1:1248818/1‑140 (MQ=255)                                                                                   acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTc                                                 >  2:1011007/1‑140 (MQ=255)                                                                                   acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTc                                                 >  1:643944/1‑140 (MQ=255)                                                                                    cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTAc                                                            >  1:620686/1‑128 (MQ=255)                                                                                    cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTAc                                                            <  2:620686/128‑1 (MQ=255)                                                                                    cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCa                                                >  2:474218/1‑140 (MQ=255)                                                                                      ccAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAAt                                              >  2:342950/1‑140 (MQ=255)                                                                                      ccAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAAt                                              <  2:1182374/140‑1 (MQ=255)                                                                                      ccAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAAt                                              >  1:1285287/1‑140 (MQ=255)                                                                                         aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTg                                           >  2:1042177/1‑140 (MQ=255)                                                                                         aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTg                                           >  2:301185/1‑140 (MQ=255)                                                                                          tCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGc                                          >  2:1319246/1‑140 (MQ=255)                                                                                          tCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGc                                          >  1:819550/1‑140 (MQ=255)                                                                                              cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAaca                                      <  2:352017/140‑1 (MQ=255)                                                                                              cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAaca                                      >  2:1174598/1‑140 (MQ=255)                                                                                                 gaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGt                                   <  2:1248818/140‑1 (MQ=255)                                                                                                      ggTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTg                              <  1:1311130/140‑1 (MQ=255)                                                                                                       gTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGt                             <  1:1096757/140‑1 (MQ=255)                                                                                                       gTTGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGt                             >  2:341050/1‑140 (MQ=255)                                                                                                        ttGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGtt                            <  1:1136802/140‑1 (MQ=255)                                                                                                        ttGATGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGtt                            <  2:881360/140‑1 (MQ=255)                                                                                                            tGTCCAGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGAtt                        >  1:917346/1‑140 (MQ=255)                                                                                                            tGTCCAGCGAACGCACTTCAATATATTAAATGCCGCC‑CGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGAttt                       >  2:356569/1‑140 (MQ=255)                                                                                                                 aGCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAg                   >  2:1069961/1‑140 (MQ=255)                                                                                                                  gCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGc                  <  2:1096288/140‑1 (MQ=255)                                                                                                                  gCGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGc                  <  1:1092944/140‑1 (MQ=255)                                                                                                                   cGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGcc                 <  2:819550/140‑1 (MQ=255)                                                                                                                   cGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGcc                 >  1:1103851/1‑140 (MQ=255)                                                                                                                   cGAACGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGcc                 >  1:665887/1‑140 (MQ=255)                                                                                                                     aaCGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctc               >  2:405445/1‑140 (MQ=255)                                                                                                                     aaCGCACTCCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctc               >  2:418729/1‑140 (MQ=255)                                                                                                                       cGCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCtt             <  1:343321/140‑1 (MQ=255)                                                                                                                        gCACTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTa            >  1:145767/1‑140 (MQ=255)                                                                                                                           cTTCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCg         <  2:348327/140‑1 (MQ=255)                                                                                                                             tCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc       <  1:515492/140‑1 (MQ=255)                                                                                                                             tCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc       <  1:1042177/140‑1 (MQ=255)                                                                                                                             tCAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc       <  2:145767/140‑1 (MQ=255)                                                                                                                              cAATATATTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCt      <  1:372240/140‑1 (MQ=255)                                                                                                                                 tatatTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtc   >  2:467889/1‑140 (MQ=255)                                                                                                                                  atatTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtct  <  1:418729/140‑1 (MQ=255)                                                                                                                                  atatTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtct  <  1:692531/140‑1 (MQ=255)                                                                                                                                  atatTAAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtct  <  2:1122189/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCT  >  NC_000913/2815333‑2815601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑