Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,810	C→A	S90I (AGC→ATC)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,810	0	C	A	100.0%	257.1 / NA	72	S90I (AGC→ATC)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/32);  total (40/32)

CGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGCTGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCGAGA  >  NC_000913/4302673‑4302942                                                                                                                                          |                                                                                                                                     cGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATg                                                                                                                                    >  2:1171781/1‑140 (MQ=255)   aCAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGcc                                                                                                                                  >  1:854156/1‑140 (MQ=255)    cAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCg                                                                                                                                 >  2:396839/1‑140 (MQ=255)    cAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCg                                                                                                                                 <  1:175800/140‑1 (MQ=255)    cAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCg                                                                                                                                 >  2:474070/1‑140 (MQ=255)    cAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCg                                                                                                                                 <  1:1036592/140‑1 (MQ=255)      aTGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGAt                                                                                                                               <  2:51579/140‑1 (MQ=255)             cGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGc                                                                                                                        <  1:481151/140‑1 (MQ=255)                 gAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCg                                                                                                                    >  2:866444/1‑140 (MQ=255)                         ccAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAAcc                                                                                                            <  2:309844/140‑1 (MQ=255)                                  aGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAg                                                                                                   >  2:298340/1‑140 (MQ=255)                                       tGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATcgc                                                                                              >  1:1221530/1‑140 (MQ=255)                                             cgcATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACaaa                                                                                        >  1:202779/1‑140 (MQ=255)                                             cgcATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACaaa                                                                                        >  1:251974/1‑140 (MQ=255)                                             cgcATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACaaa                                                                                        >  1:275251/1‑140 (MQ=255)                                             cgcATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACaaa                                                                                        >  2:1302192/1‑140 (MQ=255)                                              gcATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAAt                                                                                       >  2:630243/1‑140 (MQ=255)                                                     aaaCATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGgtgt                                                                                >  2:814787/1‑140 (MQ=255)                                                        cATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTaaa                                                                             <  1:950497/140‑1 (MQ=255)                                                          tGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAAc                                                                           >  2:1085127/1‑140 (MQ=255)                                                             aGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAAcgcg                                                                        <  1:1085127/140‑1 (MQ=255)                                                             aGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAAcgcg                                                                        <  1:474070/140‑1 (MQ=255)                                                             aGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAAcgcg                                                                        <  1:396839/140‑1 (MQ=255)                                                               cccATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGtt                                                                      <  2:1221530/140‑1 (MQ=255)                                                               cccATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGtt                                                                      <  2:843567/140‑1 (MQ=255)                                                                  aTCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGa                                                                   >  2:403237/1‑140 (MQ=255)                                                                         tCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATa                                                            >  1:697788/1‑140 (MQ=255)                                                                         tCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATa                                                            >  2:113569/1‑140 (MQ=255)                                                                           ggTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCAACTGAATAcc                                                          >  2:456427/1‑140 (MQ=255)                                                                            gTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGa                                                                   >  2:1318568/1‑130 (MQ=255)                                                                            gTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCg                                                         <  2:697788/140‑1 (MQ=255)                                                                            gTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGACAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGa                                                                   <  1:1318568/130‑1 (MQ=255)                                                                             tCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGt                                                        >  2:1152669/1‑140 (MQ=255)                                                                             tCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGt                                                        >  1:42115/1‑140 (MQ=255)                                                                              ccGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTa                                                       >  1:1154168/1‑140 (MQ=255)                                                                               cGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTaa                                                      <  1:163394/140‑1 (MQ=255)                                                                                    atgatCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAg                                                 <  2:202779/140‑1 (MQ=255)                                                                                      gatCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCa                                               <  2:854156/140‑1 (MQ=255)                                                                                       atCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGGATAc                                                           <  1:843550/127‑1 (MQ=255)                                                                                       atCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGGATAc                                                           >  2:843550/1‑127 (MQ=255)                                                                                               gATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCg                                      >  1:741826/1‑140 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAGATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGAt                                    >  1:1323226/1‑140 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCa                                               >  1:887134/1‑129 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCa                                               <  2:887134/129‑1 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGAt                                    <  1:630243/140‑1 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGAt                                    >  2:578837/1‑140 (MQ=255)                                                                                                 tCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGAt                                    <  1:1383880/140‑1 (MQ=255)                                                                                                   aaCGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATaa                                  <  2:741826/140‑1 (MQ=255)                                                                                                   aaCGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATaa                                  <  1:298340/140‑1 (MQ=255)                                                                                                      ggTTCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGc                               >  2:964086/1‑140 (MQ=255)                                                                                                         tCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCcac                            >  2:1165935/1‑140 (MQ=255)                                                                                                         tCGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCcac                            >  2:1275363/1‑140 (MQ=255)                                                                                                          cGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCcaca                           <  1:257119/140‑1 (MQ=255)                                                                                                          cGCCGTATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCcaca                           >  1:461958/1‑140 (MQ=255)                                                                                                               tATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAgg                      <  1:866444/140‑1 (MQ=255)                                                                                                               tATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAgg                      <  2:1154168/140‑1 (MQ=255)                                                                                                               tATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAgg                      <  2:275251/140‑1 (MQ=255)                                                                                                               tATGACCATTTGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAgg                      <  2:251974/140‑1 (MQ=255)                                                                                                                       tttGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGc                               >  2:730014/1‑123 (MQ=255)                                                                                                                       tttGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGc                               <  1:730014/123‑1 (MQ=255)                                                                                                                       tttGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCaa              >  1:1206616/1‑140 (MQ=255)                                                                                                                         tGTAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAg            >  2:553204/1‑140 (MQ=255)                                                                                                                           tAGATCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGt          >  2:246664/1‑140 (MQ=255)                                                                                                                              aTCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCAt       >  1:543939/1‑140 (MQ=255)                                                                                                                               tCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATc      <  1:1364956/140‑1 (MQ=255)                                                                                                                               tCAAAAACAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATc      <  1:113569/140‑1 (MQ=255)                                                                                                                                  aaaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCaa              >  2:330489/1‑129 (MQ=255)                                                                                                                                  aaaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCaa              <  1:330489/129‑1 (MQ=255)                                                                                                                                  aaaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCgag   >  2:83769/1‑140 (MQ=255)                                                                                                                                  aaaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCgag   >  2:806499/1‑140 (MQ=255)                                                                                                                                  aaaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCgag   >  1:399170/1‑140 (MQ=255)                                                                                                                                   aaaCAGGATGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCgaga  <  2:810345/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAAAAACAGGCTGCCGATCTCCAGCACCGTGGCAACCACAAACAAGATCGCGACAAATTTGGTGTAAAACGCGTTCGACTGAATACCGTAAAACAGCACTGCCAGCGATAACGCCACAAAAGGGATCTCAAAGGTCATCGAGA  >  NC_000913/4302673‑4302942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑