Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	519,680	G→T	T29K (ACG→AAG)	tesA ←	multifunctional acyl‑CoA thioesterase I and protease I and lysophospholipase L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	519,680	0	G	T	100.0%	261.4 / NA	73	T29K (ACG→AAG)	tesA	multifunctional acyl‑CoA thioesterase I and protease I and lysophospholipase L1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/31);  total (42/31)

CGAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATCCTCACCGGA  >  NC_000913/519547‑519816                                                                                                                                      |                                                                                                                                         cGAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCg                                                                                                                                    >  1:594738/1‑140 (MQ=255) cGAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTAGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCg                                                                                                                                    >  2:833252/1‑140 (MQ=255)  gAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGc                                                                                                                                   >  2:1312857/1‑140 (MQ=255)  gAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGc                                                                                                                                   >  1:159157/1‑140 (MQ=255)   aGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCt                                                                                                                                  >  2:1343286/1‑140 (MQ=255)              cTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACg                                                                                                                       >  2:255879/1‑140 (MQ=255)              cTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACg                                                                                                                       <  2:159157/140‑1 (MQ=255)                gATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGa                                                                                                                     <  2:556772/140‑1 (MQ=255)                   gCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAgg                                                                                                                  <  1:14418/140‑1 (MQ=255)                             aCTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGAc                                                                                                        <  2:950981/140‑1 (MQ=255)                                 ccGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAg                                                                                                                   >  1:939839/1‑125 (MQ=255)                                 ccGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAg                                                                                                                   <  2:939839/125‑1 (MQ=255)                                 ccGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACcagg                                                                                                    >  2:40964/1‑140 (MQ=255)                                 ccGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACcagg                                                                                                    >  2:394941/1‑140 (MQ=255)                                   gACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACcaggaa                                                                                                  >  2:1071484/1‑140 (MQ=255)                                               tGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACcagg                                                                                                    <  1:1371951/126‑1 (MQ=255)                                               tGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACcagg                                                                                                    >  2:1371951/1‑126 (MQ=255)                                               tGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCaa                                                                                      >  1:1111951/1‑140 (MQ=255)                                                       aTCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGc                                                                              >  1:1312396/1‑140 (MQ=255)                                                       aTCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGc                                                                              >  1:1293646/1‑140 (MQ=255)                                                          aTTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTAGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGa                                                                           <  1:833252/140‑1 (MQ=255)                                                           ttCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGaa                                                                          >  1:1361650/1‑140 (MQ=255)                                                           ttCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGaa                                                                          >  1:215258/1‑140 (MQ=255)                                                             caacaaGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGaaaa                                                                        <  2:215258/140‑1 (MQ=255)                                                              aacaaGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAAc                                                                       >  1:770880/1‑140 (MQ=255)                                                                caaGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACAt                                                                     >  2:5651/1‑140 (MQ=255)                                                                    gcaggcCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACAttgtt                                                                 >  2:855919/1‑140 (MQ=255)                                                                     caggccaggcCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACAttgttg                                                                >  2:22367/1‑140 (MQ=255)                                                                      aggccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGa                                                               >  1:8233/1‑140 (MQ=255)                                                                       ggccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGaa                                                              <  2:1312396/140‑1 (MQ=255)                                                                        gccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGa                                                               <  2:780948/138‑1 (MQ=255)                                                                        gccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGa                                                               >  1:780948/1‑138 (MQ=255)                                                                        gccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAg                                                             >  2:630193/1‑140 (MQ=255)                                                                        gccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAg                                                             >  1:109283/1‑140 (MQ=255)                                                                         ccaggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGt                                                            <  1:255879/140‑1 (MQ=255)                                                                            ggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtca                                                         <  2:94933/140‑1 (MQ=255)                                                                            ggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtca                                                         <  1:1312857/140‑1 (MQ=255)                                                                            ggccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtca                                                         <  1:1086969/140‑1 (MQ=255)                                                                             gccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtcat                                                        >  1:1361600/1‑140 (MQ=255)                                                                              ccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtcatc                                                       <  1:22367/140‑1 (MQ=255)                                                                              ccGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtcatc                                                       <  2:594738/140‑1 (MQ=255)                                                                                  gcTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtcatc                                                       <  2:1113794/136‑1 (MQ=255)                                                                                  gcTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtcatc                                                       >  1:1113794/1‑136 (MQ=255)                                                                                  gcTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCt                                                   >  1:152306/1‑140 (MQ=255)                                                                                  gcTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCt                                                   >  2:852001/1‑140 (MQ=255)                                                                                  gcTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCt                                                   >  2:1296901/1‑140 (MQ=255)                                                                                            aTTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTcc                                         >  1:54242/1‑140 (MQ=255)                                                                                            aTTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTcc                                         >  1:1168915/1‑140 (MQ=255)                                                                                              tCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGt                                       <  2:770880/140‑1 (MQ=255)                                                                                              tCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGt                                       <  1:196334/140‑1 (MQ=255)                                                                                              tCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGt                                       >  2:851825/1‑140 (MQ=255)                                                                                              tCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGt                                       <  1:914606/140‑1 (MQ=255)                                                                                              ccGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtca                                                         <  2:1076632/121‑1 (MQ=255)                                                                                              ccGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTtca                                                         >  1:1076632/2‑122 (MQ=255)                                                                                                  tACCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGt                                   <  2:1361600/140‑1 (MQ=255)                                                                                                   aCCCGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTc                                  <  2:8233/140‑1 (MQ=255)                                                                                                     ccGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAg                                >  1:384329/1‑140 (MQ=255)                                                                                                      cGGCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgg                               <  2:1361650/140‑1 (MQ=255)                                                                                                       ggCGCTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAggg                              >  1:1040878/1‑140 (MQ=255)                                                                                                        gcgcTCAGGCTATCACCCATAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggg                             >  2:1189818/1‑140 (MQ=255)                                                                                                        gcgcTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggg                             >  2:257765/1‑140 (MQ=255)                                                                                                        gcgcTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggg                             <  1:855919/140‑1 (MQ=255)                                                                                                        gcgcTCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggg                             >  1:743615/1‑140 (MQ=255)                                                                                                            tCAGGCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGc                         <  1:5651/140‑1 (MQ=255)                                                                                                                gCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGa                     <  1:1071484/140‑1 (MQ=255)                                                                                                                gCTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGa                     <  1:630193/140‑1 (MQ=255)                                                                                                                 cTATCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGaa                    <  2:1293646/140‑1 (MQ=255)                                                                                                                   aTCACCCAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTGAGAAGTCCGTCGGTCAGGGGGAGCATGAAct                  >  2:488125/1‑140 (MQ=255)                                                                                                                       cccAGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTcc              <  1:40964/140‑1 (MQ=255)                                                                                                                          aGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGc                         <  1:1259287/126‑1 (MQ=255)                                                                                                                          aGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGc                         >  2:1259287/1‑126 (MQ=255)                                                                                                                          aGAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATc           >  2:584913/1‑140 (MQ=255)                                                                                                                           gAATCAATAACTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATcc          >  1:911415/1‑140 (MQ=255)                                                                                                                                   aaCTTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTGAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATCCTCACCGGa  <  1:488125/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGAGGTGTCGCCGCTGATGCTGGCATTAACTACCGACGTTTTACTCTGCCACTTATCATTCAACAAGGCAGGCCAGGCCGCGCTGGCAGACATTCGATACCCGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATCCTCACCGGA  >  NC_000913/519547‑519816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑