Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108,124	A→C	V69G (GTT→GGT)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108,124	0	A	C	100.0%	237.2 / NA	64	V69G (GTT→GGT)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/32);  total (32/32)

ATTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTAACGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAATCACTAAAACTTTTTTGTT  >  NC_000913/2107988‑2108260                                                                                                                                         |                                                                                                                                         atTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGt                                                                                                                                       >  1:892993/1‑140 (MQ=255) atTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGt                                                                                                                                       >  1:2518795/1‑140 (MQ=255) atTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGt                                                                                                                                       >  1:840050/1‑140 (MQ=255)  ttttGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTa                                                                                                                                      <  2:892993/140‑1 (MQ=255)   tttGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTaa                                                                                                                                     >  1:563575/1‑140 (MQ=255)    ttGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAAt                                                                                                                                    <  2:563575/140‑1 (MQ=255)    ttGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAAt                                                                                                                                    >  2:467298/1‑140 (MQ=255)    ttGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAAt                                                                                                                                    <  2:2294433/140‑1 (MQ=255)         cttcttGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCat                                                                                                                               <  2:840050/140‑1 (MQ=255)            cttGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTa                                                                                                                                      >  1:1283536/1‑130 (MQ=255)            cttGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTa                                                                                                                                      <  2:1283536/130‑1 (MQ=255)            cttGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCatat                                                                                                                             <  1:1302698/139‑1 (MQ=255)            cttGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCatat                                                                                                                             >  2:1302698/1‑139 (MQ=255)                aGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATAtt                                                                                                                        <  1:2327732/140‑1 (MQ=255)                           cTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTa                                                                                                             <  1:1052307/140‑1 (MQ=255)                           cTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTa                                                                                                             <  1:467298/140‑1 (MQ=255)                               ccACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGaa                                                                                                         >  2:2317497/1‑140 (MQ=255)                                cacaTTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGaaa                                                                                                        >  1:1245551/1‑140 (MQ=255)                                cacaTTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGaaa                                                                                                        <  1:2317497/140‑1 (MQ=255)                                cacaTTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGaaa                                                                                                        >  1:66367/1‑140 (MQ=255)                                     ttGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAatat                                                                                                   <  1:2375253/140‑1 (MQ=255)                                       ggtggAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATAtgt                                                                                                 >  1:1236995/1‑140 (MQ=255)                                        gtggAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATAtgtg                                                                                                >  1:2348698/1‑140 (MQ=255)                                          ggAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCa                                                                                              <  1:710747/140‑1 (MQ=255)                                          ggAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCa                                                                                              <  1:1473523/140‑1 (MQ=255)                                          ggAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCa                                                                                              <  2:2348698/140‑1 (MQ=255)                                            aaaGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCAcc                                                                                            <  1:1947700/140‑1 (MQ=255)                                             aaGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCa                                                                                           <  1:615015/140‑1 (MQ=255)                                                  ttCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCAcc                                                                                            >  1:236263/1‑134 (MQ=255)                                                  ttCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCAcc                                                                                            <  2:236263/134‑1 (MQ=255)                                                          aaaaGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCt                                                                              >  1:2618567/1‑140 (MQ=255)                                                          aaaaGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCt                                                                              >  2:496227/1‑140 (MQ=255)                                                          aaaaGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCt                                                                              >  1:1584658/1‑140 (MQ=255)                                                          aaaaGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCt                                                                              >  2:1251638/1‑140 (MQ=255)                                                          aaaaGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGAACCATATTTATGAATCt                                                                              >  2:243546/1‑140 (MQ=255)                                                             aggaaggTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGa                                                                           <  2:66367/140‑1 (MQ=255)                                                                 aggTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGaa                                                                                 <  2:2163059/130‑1 (MQ=255)                                                                 aggTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGaa                                                                                 >  1:2163059/1‑130 (MQ=255)                                                                  ggTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGa                                                                                  <  2:1190497/128‑1 (MQ=255)                                                                  ggTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGa                                                                                  >  1:1190497/1‑128 (MQ=255)                                                                    ttGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTc                                                                    <  1:731296/140‑1 (MQ=255)                                                                    ttGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTc                                                                    <  2:1561459/140‑1 (MQ=255)                                                                      gAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTcac                                                                  <  1:1471243/140‑1 (MQ=255)                                                                             ttGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATAccc                                                                      <  2:617062/129‑1 (MQ=255)                                                                             ttGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATAccc                                                                      >  1:617062/1‑129 (MQ=255)                                                                             ttGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTca                                                                   <  1:2514537/132‑1 (MQ=255)                                                                             ttGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTca                                                                   >  2:2514537/1‑132 (MQ=255)                                                                             ttGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCNNNGTCctc                                                           >  1:1689993/1‑140 (MQ=255)                                                                              tGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATAccc                                                                      <  1:388235/128‑1 (MQ=255)                                                                              tGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATAccc                                                                      >  2:388235/1‑128 (MQ=255)                                                                                   ttATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTa                                                     <  2:990267/140‑1 (MQ=255)                                                                                   ttATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTa                                                     <  1:768775/140‑1 (MQ=255)                                                                                     ataAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACg                                                   >  2:2126129/1‑140 (MQ=255)                                                                                        aaTCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCAt                                                <  2:2455953/140‑1 (MQ=255)                                                                                          tCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCAttt                                              <  2:1584658/140‑1 (MQ=255)                                                                                            gCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTcc                                            >  1:799814/1‑140 (MQ=255)                                                                                               aGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCAcc                                         <  1:496227/140‑1 (MQ=255)                                                                                                 tGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATAATTGGTATGAAAAATATGTGCAGCATATTTATGAATCTGGATACCCTCACAGTCTTCTGTGTACGCATTTCCACCGa                                       >  2:2109835/1‑140 (MQ=255)                                                                                                        ttAGTAAAACGATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGAtt                                >  2:1490999/1‑140 (MQ=255)                                                                                                                  gATTAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCa                      >  1:226900/1‑140 (MQ=255)                                                                                                                    ttAAATTCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAAt                    >  2:2475017/1‑140 (MQ=255)                                                                                                                          tCTACTAAATCATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAATCACTaa              <  2:2618567/140‑1 (MQ=255)                                                                                                                                    cATTACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAATCACTAAAACTTTTTTg    >  1:2395828/1‑140 (MQ=255)                                                                                                                                      ttACCGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAATCACTAAAACTTTTTTGtt  >  2:1299864/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         ATTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTTGGTGGAAAGTATTCATATTAAAAGGAAGGTTGAATAATTTGTCTTTATAAATCGCCAGTGGAGAATTAGTAAAACGATTAAATTCTACTAAATCATTAACGTAATCCCATATATATTTATCATTGGTATGAAAAATATGTGCACCATATTTATGAATCTGGATACCCTCACAGTCCTCTGTGTACGCATTTCCACCGATATGATTTCTTTTCTCAATCACTAAAACTTTTTTGTT  >  NC_000913/2107988‑2108260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑