Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,142,795	C→A	E525* (GAA→TAA)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,142,795	0	C	A	100.0%	276.4 / NA	79	E525* (GAA→TAA)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/38);  total (41/38)

CAGCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGT  >  NC_000913/1142658‑1142927                                                                                                                                          |                                                                                                                                     cAGCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACg                                                                                                                                    <  2:770443/140‑1 (MQ=255)   gCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGa                                                                                                                                  >  2:1436906/1‑140 (MQ=255)   gCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGa                                                                                                                                  <  1:279088/140‑1 (MQ=255)    cccAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGAc                                                                                                                                 >  2:2163887/1‑140 (MQ=255)       aGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCt                                                                                                                              >  1:1434958/1‑140 (MQ=255)         gTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTa                                                                                                                            >  1:2484999/1‑140 (MQ=255)           tgtgCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACg                                                                                                                          <  1:29791/140‑1 (MQ=255)            gtgCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGt                                                                                                                         <  1:1100772/140‑1 (MQ=255)             tgCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGtt                                                                                                                        >  2:49610/1‑140 (MQ=255)             tgCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGtt                                                                                                                        >  1:134864/1‑140 (MQ=255)              gCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCCGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTc                                                                                                                       >  2:1087977/1‑140 (MQ=255)                  gAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCg                                                                                                                   <  2:749534/140‑1 (MQ=255)                  gAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCg                                                                                                                   >  1:61510/1‑140 (MQ=255)                  gAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGAAGCGGCAGGTTCAGCCCGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCg                                                                                                                   <  1:2243046/140‑1 (MQ=255)                       gctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAActc                                                                                                              >  1:85216/1‑140 (MQ=255)                        ctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAActct                                                                                                             >  1:469147/1‑140 (MQ=255)                        ctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAActct                                                                                                             <  2:2303699/140‑1 (MQ=255)                         tgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            <  1:1397881/140‑1 (MQ=255)                         tgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            <  2:1833264/140‑1 (MQ=255)                            tgtTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGc                                                                                                                    >  1:923180/1‑129 (MQ=255)                            tgtTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGc                                                                                                                    <  2:923180/129‑1 (MQ=255)                                gCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            >  1:868219/1‑133 (MQ=255)                                gCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            <  2:868219/133‑1 (MQ=255)                                gCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            >  2:1510439/1‑133 (MQ=255)                                gCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACtctt                                                                                                            <  1:1510439/133‑1 (MQ=255)                                gCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGa                                                                                                     >  1:739061/1‑140 (MQ=255)                                     tGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGcc                                                                                                >  2:612521/1‑139 (MQ=255)                                     tGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCa                                                                                                >  1:254669/1‑140 (MQ=255)                                     tGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCa                                                                                                >  1:1638498/1‑140 (MQ=255)                                              cGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCg                                                                                       <  2:61510/140‑1 (MQ=255)                                              cGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCg                                                                                       <  2:85216/140‑1 (MQ=255)                                                      gAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCa                                                                               >  1:1315049/1‑140 (MQ=255)                                                              acaGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCtt                                                                       <  2:469147/140‑1 (MQ=255)                                                              acaGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCtt                                                                       <  2:1638498/140‑1 (MQ=255)                                                                aGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCg                                                                     <  2:739061/140‑1 (MQ=255)                                                                 ggCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCgg                                                                    <  2:431088/140‑1 (MQ=255)                                                                 ggCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCgg                                                                    >  1:1131763/1‑140 (MQ=255)                                                                  gcgcGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACGTCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgc                                                                   >  1:1599612/1‑140 (MQ=255)                                                                  gcgcGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgc                                                                   >  1:1967293/1‑140 (MQ=255)                                                                  gcgcGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgc                                                                   >  2:1194282/1‑140 (MQ=255)                                                                  gcgcGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGGTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgc                                                                   >  1:1866053/1‑140 (MQ=255)                                                                   cgcgGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgca                                                                  >  2:2332285/1‑140 (MQ=255)                                                                   cgcgGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgca                                                                  >  2:1180125/1‑140 (MQ=255)                                                                   cgcgGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgca                                                                  <  2:1315049/140‑1 (MQ=255)                                                                      ggCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGgcagca                                                               <  2:1967293/140‑1 (MQ=255)                                                                             tCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCt                                                        >  2:1880530/1‑140 (MQ=255)                                                                                  tGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAgg                                                   >  2:2197661/1‑140 (MQ=255)                                                                                      gTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTgg                                               >  1:1340002/1‑140 (MQ=255)                                                                                       ttGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCTCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTggg                                              <  2:1040777/140‑1 (MQ=255)                                                                                         ggCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGt                                            >  1:1361337/1‑140 (MQ=255)                                                                                         ggCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGt                                            >  1:1759158/1‑140 (MQ=255)                                                                                          gcgcAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGtt                                           >  2:2297415/1‑140 (MQ=255)                                                                                               ggcggcACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTttcttc                                      <  2:134864/140‑1 (MQ=255)                                                                                               ggcggcACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTttcttc                                      >  2:976568/1‑140 (MQ=255)                                                                                               ggcggcACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTttcttc                                      <  1:1194282/140‑1 (MQ=255)                                                                                                    cacaTCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCtt                                 >  1:731399/1‑140 (MQ=255)                                                                                                          cGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTacgca                           >  1:1633950/1‑140 (MQ=255)                                                                                                            gCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTacgcacg                         <  2:1839679/140‑1 (MQ=255)                                                                                                            gCATGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTacgcacg                         >  2:1533466/1‑140 (MQ=255)                                                                                                              aTGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCAcgcg                       <  1:1533466/140‑1 (MQ=255)                                                                                                              aTGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCAcgcg                       <  1:1880530/140‑1 (MQ=255)                                                                                                              aTGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCAcgcg                       <  2:2520865/140‑1 (MQ=255)                                                                                                              aTGGCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCAcgcg                       <  2:297982/140‑1 (MQ=255)                                                                                                                ggCAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgca                     <  2:1131763/140‑1 (MQ=255)                                                                                                                  cAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgcagc                   >  2:1663322/1‑140 (MQ=255)                                                                                                                  cAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgcagc                   >  1:1310307/1‑140 (MQ=255)                                                                                                                  cAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgcagc                   <  2:2484999/140‑1 (MQ=255)                                                                                                                  cAAAGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgcagc                   >  1:2598284/1‑140 (MQ=255)                                                                                                                   aaaGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgcagca                  <  2:731399/140‑1 (MQ=255)                                                                                                                     aGGTTGCCAGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACg                <  1:1087977/140‑1 (MQ=255)                                                                                                                             aGCGCAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTg        <  2:1310307/140‑1 (MQ=255)                                                                                                                               cgcAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgca                     <  2:2655637/125‑1 (MQ=255)                                                                                                                               cgcAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCgca                     >  1:2655637/1‑125 (MQ=255)                                                                                                                               cgcAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCg      <  2:1759158/140‑1 (MQ=255)                                                                                                                                gcAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCgg     >  2:222555/1‑140 (MQ=255)                                                                                                                                gcAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCgg     >  2:1377084/1‑140 (MQ=255)                                                                                                                                 cAGGTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCggg    >  1:2718306/1‑140 (MQ=255)                                                                                                                                   ggTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGt  <  1:1663322/140‑1 (MQ=255)                                                                                                                                   ggTTGTTACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGt  <  2:1633950/140‑1 (MQ=255)                                                                                                                                    gttgttACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCg      >  1:2518492/1‑135 (MQ=255)                                                                                                                                    gttgttACGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCg      <  2:2518492/135‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CAGCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGT  >  NC_000913/1142658‑1142927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑