Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,640	0	T	G	25.0%	87.8 / 12.9	44	L176V (TTA→GTA)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (10/23);  new base G (11/0);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.60e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779                                                                                                                                              |                                                                                                                                            cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGt                                                                                                                                             >  1:696119/1‑140 (MQ=255) cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGt                                                                                                                                             <  1:381532/140‑1 (MQ=255)              cTTCCGGCGCTGGAAGTGGTGGGAAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGtt                                                                                                                                            >  1:112775/1‑128 (MQ=255)              cTTCCGGCGCTGGAAGTGGTGGGAAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGtt                                                                                                                                            <  2:112775/128‑1 (MQ=255)                tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAg                                                                                                                                          <  1:1113054/128‑1 (MQ=255)                tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAg                                                                                                                                          >  2:1113054/1‑128 (MQ=255)                tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGaaaaaat                                                                                                                              >  1:188311/1‑138 (MQ=255)                tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACt                                                                                                                              >  1:918761/1‑140 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGCGGGGGTTGAACCTaaaaaaagcgc                                                                                                                           >  2:677093/1‑135 (MQ=255)                       cTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCAt                                                                                                                       <  2:614021/140‑1 (MQ=255)                         ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTTGACCTGAAAAAATGCGCCATGa                                                                                                                     >  2:1148976/1‑140 (MQ=255)                            aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGa                                                                                                                  <  1:420450/140‑1 (MQ=255)                            aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGa                                                                                                                  >  1:559485/1‑140 (MQ=255)                               ggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGa                                                                                                               <  2:464229/140‑1 (MQ=255)                                 tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  1:325303/140‑1 (MQ=255)                                 tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  1:314285/140‑1 (MQ=255)                                   gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                           <  2:918761/140‑1 (MQ=255)                                       gCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaat                                                                                                       <  2:1144916/140‑1 (MQ=255)                                       gCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaat                                                                                                       >  1:472514/1‑140 (MQ=255)                                        ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAAATGCGCCCTGAAGATGACGCGtaata                                                                                                      >  2:377099/1‑140 (MQ=255)                                        ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAAATGCGCCATGAAGATGACGCGgaata                                                                                                      >  2:132270/1‑140 (MQ=255)                                        ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGCCCGGCGCAAAGGCCGGGC‑GGGGTTGGGCTTAAAAAAAT‑CGCCATGAAGATGACCCGgaata                                                                                                      >  2:439142/1‑140 (MQ=255)                                             ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaa                                                                                                        <  2:1202031/133‑1 (MQ=255)                                             ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaa                                                                                                        >  1:1202031/1‑133 (MQ=255)                                                  gACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑CGC‑GGGGGTAGACCCGAAAAAATGCGCCATGAAGATGACGCGCAAAAACGAAGAggg                                                                                            >  2:837578/1‑139 (MQ=255)                                                        tCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAg                                                                                      >  1:1222880/1‑140 (MQ=255)                                                            ttCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCggg                                                                                  <  2:670521/140‑1 (MQ=255)                                                                      cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  2:404608/140‑1 (MQ=255)                                                                      cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCg                                                                        >  2:102367/1‑140 (MQ=255)                                                                       gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       <  2:696119/140‑1 (MQ=255)                                                                       gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       <  2:472514/140‑1 (MQ=255)                                                                       gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       <  2:1222880/140‑1 (MQ=255)                                                                              tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                                >  1:854724/1‑140 (MQ=255)                                                                              tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                                >  1:688088/1‑140 (MQ=255)                                                                               ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGgcg                                                                                <  2:600729/123‑1 (MQ=255)                                                                               ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGgcg                                                                                >  1:600729/1‑123 (MQ=255)                                                                               ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTTTAGCGGGCGCGGCAGCGAAAGCCTgg                                                               >  2:936777/1‑140 (MQ=255)                                                                                      aaCGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATcc                                                        >  1:1072102/1‑140 (MQ=255)                                                                                      aaCGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATcc                                                        >  1:104891/1‑140 (MQ=255)                                                                                                gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                              <  1:936777/140‑1 (MQ=255)                                                                                                gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                              <  1:677093/140‑1 (MQ=255)                                                                                                     gtgtATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGt                                         <  1:993907/140‑1 (MQ=255)                                                                                                        tATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:1093253/1‑140 (MQ=255)                                                                                                               tCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                               <  2:841876/140‑1 (MQ=255)                                                                                                               tCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGTTTTTAGCGGGCGCGGCAGCGGATGCCTGGGGCATCCGCTTAAAGCGGCCGTctggctggga                               >  2:861925/1‑138 (MQ=255)                                                                                                                cggcggTCCGGTGCAACGTCC‑GGC‑GGGGTTAAGCCTGGAAAACTGCGCCATGAAGATTACGGGTAATAACGAAGAGGTTGTTAGCGGGGGCGGCAGCGAATGCCTGGGACATCCGCTTAAAGCGGCCGTCTGGGTGGCAc                              >  2:993907/1‑140 (MQ=255)                                                                                                                cggcggTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              <  2:260882/140‑1 (MQ=255)                                                                                                                cggcggTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              >  1:1130272/1‑140 (MQ=255)                                                                                                                  gtggTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc                            >  1:902024/3‑140 (MQ=255)                                                                                                                  gcggTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc                            <  2:1093253/140‑1 (MQ=255)                                                                                                                     gTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaa                         >  1:648291/1‑140 (MQ=255)                                                                                                                        cGGCGCAACGTCC‑GGC‑GGGGGTAAACCTGCAAAACTGCCCCCTGAAGATGACGCGTAATAAAGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGGCTTGGACATCCGCTTTAAGGGGCCGTCTTGCTGGCACGCaaaaag                      >  2:636759/1‑138 (MQ=255)                                                                                                                                cGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa                          <  2:277945/128‑1 (MQ=255)                                                                                                                                cGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa                          >  1:277945/1‑128 (MQ=255)                                                                                                                                        c‑ggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGaa       <  1:837578/140‑1 (MQ=255)                                                                                                                                            ggTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCg    >  2:1650/1‑140 (MQ=255)                                                                                                                                              ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  >  2:322547/1‑140 (MQ=255)                                                                                                                                              ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  2:218599/140‑1 (MQ=255)                                                                                                                                              ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  2:110231/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                            CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCC‑GGC‑GGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑