Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA34.6% 34.5 / 10.6 26intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (17/0);  new base A (0/9);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 3.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT  >  NC_000913/1824922‑1825195
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tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                        >  1:326876/1‑140 (MQ=255)
         aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                               >  1:1004109/1‑140 (MQ=255)
         aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                               >  1:297892/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                             >  2:901751/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                             >  1:1154887/1‑140 (MQ=255)
             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                           >  1:393892/1‑140 (MQ=255)
                  ccGCCCCCGGCCCTTTTTTTTAATTTTTTTCTTTCAGTTACCCGTGGATGTTTATCGGCACCTGGGGTAATTTACTTTGGTGGTTATCAGTTTTTTTTAACACTTGTTTAAAAAAAAAAAAAAAAACCGCCGATCCTGTc                                                                                                                      <  2:1004109/139‑1 (MQ=255)
                          gccccTTTCTTTTCTTTGTTATCGTCAGGGTAACGGGGGTTGTTTAGGGGAATCTGGGGTGATTTAGGTAGGGGGTGAACGGGTTTTTTTCACAGTTGTTAGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                              <  2:297892/138‑1 (MQ=255)
                          gccccTTTCTTTTAATTGTTTTCGTTCAGTTAACGGTGGATGGTTATCGGCACCGGGGTTGATTTACGTAAGTGGTGATCAGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                              <  2:393892/138‑1 (MQ=255)
                            cccTTTTTTTTCTTTTTTATCTTTCAGGTAACGGTGGATGTTTATGGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCActaccttg                                                                                                            <  2:1222635/140‑8 (MQ=255)
                            cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                            >  2:699524/1‑140 (MQ=255)
                                 ttttttCTTTTTTATCTTTCGGGTAACGGTGGTTGTTTACCGGCAACTGGGGTGTTTTGTGTTGGTGGTGATCGGTTTTTTTTTACAACTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                       <  2:597245/138‑1 (MQ=255)
                                    tttCATGTTTTTCTTGCAGTTACCCGTGGATGTTTATCGGCACCGCGGGTAATTTTGGTTGGGGGTGATCGGCTTTTTTTTACATCTTTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                    <  2:1154887/140‑1 (MQ=255)
                                            ttATCGTTAAGGTAACGGTGGTTGTTTACCGGCACCTGGGGTGATTTAGGTTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                            <  2:1108447/140‑1 (MQ=255)
                                              aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAcct                                                                                          >  1:597245/1‑138 (MQ=255)
                                                      ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                  >  1:1046548/1‑140 (MQ=255)
                                                      ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                  >  1:115450/1‑140 (MQ=255)
                                                            cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                            >  1:780848/1‑140 (MQ=255)
                                                                            catctggggTGATTTCCGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                            <  1:901751/134‑1 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGat                                                         >  2:658206/1‑140 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                         >  1:220877/1‑140 (MQ=255)
                                                                                              ttAGTGGTGATCAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                          <  1:1191642/140‑1 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTTTTCAGCAGTTGCAGGCa                                      >  1:691608/1‑140 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                      >  2:836217/1‑140 (MQ=255)
                                                                                                         cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                               >  1:827232/1‑140 (MQ=255)
                                                                                                              tttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGATGAAAAATTCTTCTGCAGGTGGGGCCATTTTCCCttttt                          >  2:974014/1‑139 (MQ=255)
                                                                                                                   ttACACCTTTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                     <  2:220877/140‑1 (MQ=255)
                                                                                                                      cAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCTGTAAGTTTTTTGGCTGAAAGATTATTTAGCCATTGCAGGGATTTTACCCTTTTCCGCTggg                  >  2:199658/1‑139 (MQ=255)
                                                                                                                                      gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt  >  1:979118/1‑140 (MQ=255)
                                                                                                                                      gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt  >  2:947824/1‑140 (MQ=255)
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TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT  >  NC_000913/1824922‑1825195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑