Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,885	C→A	A176S (GCT→TCT)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,885	0	C	A	100.0%	172.5 / NA	49	A176S (GCT→TCT)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/21);  total (28/21)

TACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTT  >  NC_000913/2260753‑2261016                                                                                                                                     |                                                                                                                                    ttttttCGGGGGTCGCTGTTTGAGCTTGGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTc                                                                                                                              <  2:1008714/137‑1 (MQ=255)  aCTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCg                                                                                                                             >  2:885449/1‑140 (MQ=255)        aGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGAt                                                                                                                       >  1:779021/1‑140 (MQ=255)        aGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGAt                                                                                                                       >  2:148412/1‑140 (MQ=255)        aGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGAt                                                                                                                       >  2:399450/1‑140 (MQ=255)          tGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATcc                                                                                                                     >  1:404603/1‑140 (MQ=255)             tCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACt                                                                                                                  >  2:608263/1‑140 (MQ=255)             tCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACt                                                                                                                  >  2:470682/1‑140 (MQ=255)                ccGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCg                                                                                                               <  1:371026/140‑1 (MQ=255)                      gAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCt                                                                                                         >  2:669892/1‑140 (MQ=255)                          ttaccccGCCGGTTCATACGGCGTTGCTTCAGCAACCGTTTTATCCAGTTCCTGCGCGGTTTTCTCCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCGCTTCGATATCTgccg                                                                                                     <  2:1289883/136‑1 (MQ=255)                             gCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCca                                                                                                  <  2:404603/140‑1 (MQ=255)                             gCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCca                                                                                                  >  1:982259/1‑140 (MQ=255)                                  ccgGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCcaca                                                                                                <  2:1286136/137‑1 (MQ=255)                                  ccgGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCcaca                                                                                                >  1:1286136/1‑137 (MQ=255)                                  ccgGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc                                                                                             <  1:847169/140‑1 (MQ=255)                                   cgGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCca                                                                                                  <  2:171929/134‑1 (MQ=255)                                   cgGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCca                                                                                                  >  1:171929/1‑134 (MQ=255)                                                    cTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                                                                           >  2:149920/1‑140 (MQ=255)                                                    cTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                                                                           <  1:317276/140‑1 (MQ=255)                                                    cTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                                                                           >  2:1345270/1‑140 (MQ=255)                                                    cTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                                                                           <  1:1123192/140‑1 (MQ=255)                                                      tCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc                                                                         <  2:982259/140‑1 (MQ=255)                                                      tCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc                                                                         <  2:953703/140‑1 (MQ=255)                                                      tCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc                                                                         <  1:399450/140‑1 (MQ=255)                                                      tCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc                                                                         <  1:757935/140‑1 (MQ=255)                                                         gCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGActtc                                                                      >  2:847334/1‑140 (MQ=255)                                                           aaCCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGTcttctt                                                                    <  1:470682/140‑1 (MQ=255)                                                             ccGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCg                                                                  <  1:885449/140‑1 (MQ=255)                                                             ccGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCg                                                                  >  1:474358/1‑140 (MQ=255)                                                              cGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCgg                                                                 >  1:959575/1‑140 (MQ=255)                                                              cGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCAATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCgg                                                                 <  2:779021/140‑1 (MQ=255)                                                               gCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg                                                                <  1:149920/140‑1 (MQ=255)                                                                   tATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg                                                            <  1:847334/140‑1 (MQ=255)                                                                     tCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAt                                                          <  1:608263/140‑1 (MQ=255)                                                                         gTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCa                                                      <  1:669892/140‑1 (MQ=255)                                                                               gcgcGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTAccc                                                >  2:1319762/1‑140 (MQ=255)                                                                                    gTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGcc                                           >  1:373385/1‑140 (MQ=255)                                                                                       ttcttcAGCGCCAGACCGGTAGAGGTACTATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAAc                                        >  2:275229/1‑140 (MQ=255)                                                                                          ttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGa                                     >  2:671935/1‑140 (MQ=255)                                                                                               cgcCAGACCGGTAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCAc                                >  1:996658/1‑140 (MQ=255)                                                                                                          tAGAGGTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACt                     <  1:148412/140‑1 (MQ=255)                                                                                                               gcaCGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcac                >  1:989859/3‑140 (MQ=255)                                                                                                               gTACGATACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcac                >  1:486595/1‑140 (MQ=255)                                                                                                                    aTACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcacccacc           >  1:653338/1‑140 (MQ=255)                                                                                                                    aTACATCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcacccacc           >  2:828378/1‑140 (MQ=255)                                                                                                                        aTCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGcc       >  2:1106795/1‑140 (MQ=255)                                                                                                                         tCGGTTTACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCa      >  1:536308/1‑140 (MQ=255)                                                                                                                             tttACCAGAAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGtt  >  2:1115466/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    TACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTT  >  NC_000913/2260753‑2261016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑