Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,684,131	G→T	intergenic (+75/+123)	yphH → / ← glyA	putative DNA‑binding transcriptional regulator, NAGC‑like/serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,684,131	0	G	T	100.0%	178.7 / NA	51	intergenic (+75/+123)	yphH/glyA	putative DNA‑binding transcriptional regulator, NAGC‑like/serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/29);  total (22/29)

GCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCG  >  NC_000913/2683997‑2684267                                                                                                                                       |                                                                                                                                         gcACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTAt                                                                                                                                     <  1:1271930/140‑1 (MQ=255) gcACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTAt                                                                                                                                     <  1:327519/140‑1 (MQ=255)  cacaACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATc                                                                                                                                    >  2:200400/1‑140 (MQ=255)  cacaACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATc                                                                                                                                    >  1:634479/1‑140 (MQ=255)   acaacaGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCa                                                                                                                                   <  2:951007/140‑1 (MQ=255)   acaacaGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCa                                                                                                                                   <  1:200400/140‑1 (MQ=255)       caGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCATTTACGACATATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTTCTCTTATCAGGcc                                                                                                                               <  2:1188102/140‑1 (MQ=255)       caGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGcc                                                                                                                               <  2:788207/140‑1 (MQ=255)          gTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTac                                                                                                                            >  1:981146/1‑140 (MQ=255)          gTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTac                                                                                                                            <  2:679461/140‑1 (MQ=255)                   aTTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGAtt                                                                                                                   <  1:404129/140‑1 (MQ=255)                       gCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCaa                                                                                                               <  1:1214817/140‑1 (MQ=255)                          aTTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAatat                                                                                                            >  2:341780/1‑140 (MQ=255)                            ttGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAGCGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAatatat                                                                                                          <  1:948899/140‑1 (MQ=255)                              gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGga                                                                                                                       >  2:807224/1‑125 (MQ=255)                              gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGga                                                                                                                       <  1:807224/125‑1 (MQ=255)                                 tGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAAt                                                                                                     >  1:824090/1‑140 (MQ=255)                                   ttGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt                                                                                                   >  2:1220713/1‑140 (MQ=255)                                    tGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTg                                                                                                  <  1:1220713/140‑1 (MQ=255)                                      aGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                                                                >  2:330745/1‑140 (MQ=255)                                      aGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                                                                <  1:1181234/140‑1 (MQ=255)                                      aGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                                                                >  1:233763/1‑140 (MQ=255)                                        gcgcAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa                                                                                              >  1:594516/1‑140 (MQ=255)                                        gcgcAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa                                                                                              >  2:1306617/1‑140 (MQ=255)                                         cgcAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAg                                                                                             <  1:341780/140‑1 (MQ=255)                                         cgcAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAg                                                                                             >  2:204851/1‑140 (MQ=255)                                                   aCAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGc                                                                                   <  2:476409/140‑1 (MQ=255)                                                      gATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCgg                                                                                >  2:1347124/1‑140 (MQ=255)                                                              ggCGCGATAA‑GTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGc                                                                                   <  1:625112/128‑1 (MQ=255)                                                              ggCGCGATAA‑GTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGc                                                                                   >  2:625112/1‑128 (MQ=255)                                                                 gcgATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTc                                                                     >  1:91198/1‑140 (MQ=255)                                                                          tAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGTCTGATGCGCTACTCTTATCAGGCCTACATGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                            <  1:1235437/140‑1 (MQ=255)                                                                          tAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                            <  1:1306617/140‑1 (MQ=255)                                                                                   ttCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa                                                   <  2:923921/140‑1 (MQ=255)                                                                                         aaggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACg                                             <  2:343524/140‑1 (MQ=255)                                                                                               gCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg                                           >  2:134377/1‑136 (MQ=255)                                                                                               gCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg                                           <  1:134377/136‑1 (MQ=255)                                                                                                ccTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca                                       <  1:110400/139‑1 (MQ=255)                                                                                                ccTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca                                       >  2:110400/1‑139 (MQ=255)                                                                                                     atgatCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACa                                 <  2:351086/140‑1 (MQ=255)                                                                                                       gatCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGc                               <  1:118237/140‑1 (MQ=255)                                                                                                        atCATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCa                              <  2:91198/140‑1 (MQ=255)                                                                                                          cATAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCaaa                            >  2:877068/1‑140 (MQ=255)                                                                                                            tAAACGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATc                          <  1:204851/140‑1 (MQ=255)                                                                                                               aCGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAAt                           >  1:914581/1‑136 (MQ=255)                                                                                                               aCGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAAt                           <  2:914581/136‑1 (MQ=255)                                                                                                                cGAAAAATTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCg                      >  2:33278/1‑140 (MQ=255)                                                                                                                   aaaaTTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGttt                   >  2:552403/1‑140 (MQ=255)                                                                                                                   aaaaTTGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGttt                   <  2:634479/140‑1 (MQ=255)                                                                                                                       ttGCCTGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCt               <  1:877068/140‑1 (MQ=255)                                                                                                                            tGATGCGCTACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGc          <  2:981146/140‑1 (MQ=255)                                                                                                                                    tACTCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCg  >  1:1162822/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         GCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCG  >  NC_000913/2683997‑2684267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑