Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,126,147	G→T	L222M (CTG→ATG)	priA ←	primosome factor N'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,126,147	0	G	T	100.0%	310.9 / NA	88	L222M (CTG→ATG)	priA	primosome factor N'
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/49);  total (39/49)

TCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCT  >  NC_000913/4126011‑4126283                                                                                                                                         |                                                                                                                                         tCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCcatcag                                                                                                                                       >  2:1263350/1‑140 (MQ=255) tCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCcatcag                                                                                                                                       >  2:2266570/1‑140 (MQ=255)  cACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCatcagc                                                                                                                                      <  1:1372817/140‑1 (MQ=255)    cGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGTACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCa                                                                                                                                    <  1:317975/140‑1 (MQ=255)    cGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCa                                                                                                                                    <  2:1804795/140‑1 (MQ=255)      aaaaCGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAgg                                                                                                                                  <  1:405246/140‑1 (MQ=255)            ggCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGaaa                                                                                                                            <  2:343149/140‑1 (MQ=255)                aTAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGt                                                                                                                        <  1:772101/140‑1 (MQ=255)                  aGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTAt                                                                                                                      <  2:652644/140‑1 (MQ=255)                      tGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGc                                                                                                                  >  1:1963723/1‑140 (MQ=255)                       gCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 <  1:594283/140‑1 (MQ=255)                       gCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 <  1:1761061/140‑1 (MQ=255)                       gCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 <  1:1263350/140‑1 (MQ=255)                       gCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 <  2:1245286/140‑1 (MQ=255)                             tCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 <  2:2027238/134‑1 (MQ=255)                             tCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGcc                                                                                                                 >  1:2027238/1‑134 (MQ=255)                             tCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTa                                                                                                           >  2:811895/1‑140 (MQ=255)                             tCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTa                                                                                                           >  2:2245057/1‑140 (MQ=255)                              cAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTAt                                                                                                          >  1:1096106/1‑140 (MQ=255)                              cAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTAt                                                                                                          >  1:890358/1‑140 (MQ=255)                                     aTTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTgc                                                                                                   >  1:1445737/1‑140 (MQ=255)                                     aTTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTgc                                                                                                   >  1:931323/1‑140 (MQ=255)                                        tCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGcc                                                                                                <  2:2256986/140‑1 (MQ=255)                                            gCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAAcg                                                                                            >  1:823772/1‑140 (MQ=255)                                               ccATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGGATTGCGCCAAcggcg                                                                                         >  1:1709833/1‑140 (MQ=255)                                               ccATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAAcggcg                                                                                         <  1:811895/140‑1 (MQ=255)                                               ccATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAAcggcg                                                                                         <  1:1304626/140‑1 (MQ=255)                                                  tCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAAcggcgtgg                                                                                      >  1:675695/1‑137 (MQ=255)                                                  tCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTg                                                                                      >  2:1231746/1‑140 (MQ=255)                                                  tCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTg                                                                                      <  2:775072/140‑1 (MQ=255)                                                    aCCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGc                                                                                    <  2:931323/140‑1 (MQ=255)                                                      cAGCGCCTGTTTGCCCTGAGCGAGCCCGTTTTCCAGTACGCTGAGATAACCCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCt                                                                                  <  2:1593171/140‑1 (MQ=255)                                                      cAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCt                                                                                  <  2:823772/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGGATTGCGCCAACGGCGGTGGCCTGtt                                                                               <  2:1709833/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCt                                                                                  >  2:1147397/1‑137 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCt                                                                                  <  1:1147397/137‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGtt                                                                               <  1:1231746/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGtt                                                                               <  2:1753734/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGtt                                                                               <  2:489674/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGtt                                                                               <  1:2245057/140‑1 (MQ=255)                                                         cgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCCGtt                                                                               >  1:914517/1‑140 (MQ=255)                                                           ccTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCg                                                                             >  1:2106608/1‑140 (MQ=255)                                                             tGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGt                                                                           <  2:890358/140‑1 (MQ=255)                                                             tGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGt                                                                           >  2:109193/1‑140 (MQ=255)                                                               tttGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTAt                                                                         <  2:1109951/140‑1 (MQ=255)                                                                      tGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCg                                                                  >  1:1994837/1‑140 (MQ=255)                                                                         gcgagcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCaa                                                               >  2:2066127/1‑140 (MQ=255)                                                                            agcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCg                                                            <  1:66484/140‑1 (MQ=255)                                                                            agcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCg                                                            <  2:1963723/140‑1 (MQ=255)                                                                             gcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGc                                                           <  2:1662854/140‑1 (MQ=255)                                                                              cACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCCGTTCGGGATTCAATCGCAACCGcc                                                          >  2:686499/1‑139 (MQ=255)                                                                               aCGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTc                                                         <  1:1716984/140‑1 (MQ=255)                                                                               aCGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTc                                                         <  1:622339/140‑1 (MQ=255)                                                                                 gTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCAc                                                       <  1:2123184/140‑1 (MQ=255)                                                                                  ttttCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCAcc                                                      <  1:2266570/140‑1 (MQ=255)                                                                                   tttCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCACTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCa                                                     >  1:2340203/1‑140 (MQ=255)                                                                                    ttCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAg                                                    <  2:1096106/140‑1 (MQ=255)                                                                                      ccAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGaa                                                  <  2:2106608/140‑1 (MQ=255)                                                                                           aCGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGc                                             >  1:794523/1‑140 (MQ=255)                                                                                             gCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCAt                                           <  2:794523/140‑1 (MQ=255)                                                                                               tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTTGGGATTTAATCGCAACCGCTCACCCGAAACGGCATAg                                         >  2:1627236/1‑140 (MQ=255)                                                                                               tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAg                                         >  2:425831/1‑140 (MQ=255)                                                                                               tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAg                                         >  2:448492/1‑140 (MQ=255)                                                                                               tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGGATTTAATCGCAACCGCTCACCCGAAACGGCATAg                                         >  2:1506754/1‑140 (MQ=255)                                                                                               tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGGGGGCGGGTCGGGATTTAAACGCAACCGCCCACCAAAAACGGCATaa                                         >  2:1556441/1‑139 (MQ=255)                                                                                                 agaTAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgtt                                       >  1:1658036/1‑140 (MQ=255)                                                                                                       aCCTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGGGGCCTGGTCGGGATTCAATCGCAACCGCTCACCCGAAACGGCATAggtcgttcc                                 >  2:201153/1‑139 (MQ=255)                                                                                                         cTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGcc                               <  1:1627236/140‑1 (MQ=255)                                                                                                         cTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGcc                               <  1:2066127/140‑1 (MQ=255)                                                                                                         cTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGcc                               <  1:1556441/140‑1 (MQ=255)                                                                                                         cTCCGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGcc                               <  1:1760631/140‑1 (MQ=255)                                                                                                           ccGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAg                             >  1:1627611/1‑140 (MQ=255)                                                                                                           ccGTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGAGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAg                             >  2:51916/1‑140 (MQ=255)                                                                                                             gTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGGCTGTTTGGGGTTTAAACGCAAACCCTCACCCGAAACGGGATAGTTCGTTCGCCCGTc                           >  2:1760631/1‑140 (MQ=255)                                                                                                             gTTTTACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGGATTTAATCGCAACCGCTCACCCGAAAAGGCATAGTTCGTTCGCCAGTc                           >  2:2338578/1‑140 (MQ=255)                                                                                                                ttACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCt                        <  2:1658036/140‑1 (MQ=255)                                                                                                                ttACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCt                        >  2:1806498/1‑140 (MQ=255)                                                                                                                 tACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTa                       <  1:686499/140‑1 (MQ=255)                                                                                                                 tACCGGAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTa                       <  2:675695/140‑1 (MQ=255)                                                                                                                      gAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAActc                  <  1:2338578/140‑1 (MQ=255)                                                                                                                      gAACCGGTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAActc                  >  2:1800409/1‑140 (MQ=255)                                                                                                                           ggTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGgtg             <  1:109193/140‑1 (MQ=255)                                                                                                                            gTAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGgtgt            >  2:1073088/1‑140 (MQ=255)                                                                                                                             tAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGtt           <  1:1806498/140‑1 (MQ=255)                                                                                                                                cgcccgccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCa        <  1:17071/140‑1 (MQ=255)                                                                                                                                 gcccgccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGGATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCCCCAGTCGCTAAAATCTTGTGGTTCAc       >  2:361420/1‑140 (MQ=255)                                                                                                                                    cgccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTg    >  2:2147455/1‑140 (MQ=255)                                                                                                                                    cgccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTg    <  2:1994837/140‑1 (MQ=255)                                                                                                                                    cgccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGAGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTg    <  1:51916/140‑1 (MQ=255)                                                                                                                                     gccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGgtg             >  2:98776/1‑130 (MQ=255)                                                                                                                                     gccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGgtg             <  1:98776/130‑1 (MQ=255)                                                                                                                                      ccATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGGCTGTTGCGTATTTAATCGCAAACGCTCACCAGAAACGGCATAGTTCGTTCCCCTGTCGCTTAACTCTGGTGTTTCCCTTGCt  >  2:17071/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCT  >  NC_000913/4126011‑4126283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑