Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,550,663	0	A	C	28.4%	149.0 / 21.0	74	N68T (AAC→ACC)	fdnH	formate dehydrogenase N subunit beta
Reads supporting (aligned to +/- strand):  ref base A (40/13);  new base C (0/21);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 6.13e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCT  >  NC_000913/1550529‑1550802                                                                                                                                       |                                                                                                                                            gCGTGATTAAAAAGCAGAAGTCGAAAAACTTATCGCCGTTCCCACCTGTATCGGCTGAAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTGGGGTTTTACGATAACCccg                                                                                                                                        <  2:360118/140‑1 (MQ=25)  cGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCccgc                                                                                                                                       >  2:1386839/1‑140 (MQ=255)    tGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGGGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCccgccg                                                                                                                                     >  1:952320/1‑140 (MQ=255)    tGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCccgccg                                                                                                                                     >  1:685589/1‑140 (MQ=255)             aGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAgcg                                                                                                                            >  2:1329687/1‑140 (MQ=255)               cAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGcc                                                                                                                          >  1:1350286/1‑140 (MQ=255)               cAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGcc                                                                                                                          >  1:1581761/1‑140 (MQ=255)               cAGAAGTCGAAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCGGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:238141/140‑1 (MQ=255)               aaaaaGTCGAAAACCTTATAGACGTTTCCCCCTGTATCGGCTATAAAGCCTGTCAGGTGGCTTGTTCGGAGTGGACCGCCACCCGTGATGAGGTGGGGCACTGCGGCGGGGTTTACAATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:1111563/137‑1 (MQ=255)               aaGAAGTCGCAAAACTTATCGACTTTTCCCCCTGTATCGGCTGTAAGGCCTGTAAGGTGGCGTGTTGGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGTTTTACGATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:764149/139‑1 (MQ=255)               aaGAAGTCGAAAAACTTATCGACTTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTGGGAGTGGAACGACACCGGTGATGAGGGGGGGCACTGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:15040/139‑1 (MQ=21)               aaGAAGTCGAAAAACTTATCGACGTTTCCCCCTGTATCGGCTGTAAAGCCTGTCAGGGGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGTTTTACGATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:246821/139‑1 (MQ=25)               aaGAAGTCAAAAAATTTATCGACTTTTCCCCCTTTATCGGCTGTAAAGCCTGTCGGGGGGCGTGTTCGGAGTGGAACGACATCCGTGATGAGGGGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:994844/139‑1 (MQ=25)               aaGAAGGCGAAAAACTAATCGGCGTTCCCACCTGTATCGGCTGAAAAGCCTGTCGGGTGGCGTGTTCGGGGTGAAACGCCATCCGTGATGAAGGGGGCCACTGCGGCGGGTTTTACAATACCCCCGCCGATCTGAGCGcc                                                                                                                          <  2:149100/139‑1 (MQ=255)                aGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCa                                                                                                                         >  1:764149/1‑140 (MQ=255)                 gAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAA‑CCCGCCGATCTGAGCGCCAAg                                                                                                                       >  1:735830/1‑140 (MQ=255)                  aaGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAg                                                                                                                       <  1:1386839/140‑1 (MQ=255)                    gccGCAAAACTTATCGACTTTTCCCCCTGTATCGGCTTAAAAGCCTGTGGGGTGGCGTGTTGGGAGTGGAACGACATCCGTGATGAGGGGGGGCACTGCGTGGGGTTTAACAATACCCCCGCCGATCTGAGCGCCAAGTc                                                                                                                     <  2:1581761/138‑1 (MQ=255)                     tCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTcc                                                                                                                    >  2:388809/1‑140 (MQ=255)                       gCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTg                                                                                                                  >  1:78551/1‑140 (MQ=255)                         aaaaCTTATCGACTTTTCCCCCTGTATCGGCTGAAAAGCCTGTCAGGTGGCGTGTTCGGAGGGGAACGACATCCGTGATGAAGTGGGGCACTGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGa                                                                                                                <  2:422171/140‑1 (MQ=37)                         aaaaCTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGa                                                                                                                >  1:994844/1‑140 (MQ=255)                                aTCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGAt                                                                                                         >  2:1109741/1‑140 (MQ=255)                                aTCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGAt                                                                                                         >  1:441809/1‑140 (MQ=255)                                           ccccTGTATGGGCTGAAAAGCTTGTCGGGGGGCGTGTGGGGAGTGGAACGCCATCCGTGATGAGGGGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCg                                                                                              <  2:850045/138‑1 (MQ=255)                                                gTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAAcc                                                                                         >  1:701057/1‑140 (MQ=255)                                                 tATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCg                                                                                        >  2:1611848/1‑140 (MQ=255)                                                   tCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGaa                                                                                      >  2:1568766/1‑140 (MQ=255)                                                   tCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGaa                                                                                      <  1:1611848/140‑1 (MQ=255)                                                   ccGGCTGTAAAGCCTTTCAGGGGGCGTGTTCGGTGTGGAACGACATCCGTGATGAGGGGGGGCACTGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGaa                                                                                      <  2:1368098/139‑1 (MQ=255)                                                        tGTAAAGCTTGTCAGGGGGCGTGTTGGGAGTGGAACGACATCGGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGaa                                                                                 <  2:588266/140‑1 (MQ=255)                                                          tAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACg                                                                               >  2:567585/1‑140 (MQ=255)                                                           aaaGCCTGTCGGGTGGGGTGTTGGGAGTGGAACGACACCGGTGATGAGGGGGGGCAGGGGGGGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACgg                                                                              <  2:685589/140‑1 (MQ=255)                                                           aaaGCCTGTCGGGTGGCGTGTTGGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACgg                                                                              <  2:126445/140‑1 (MQ=255)                                                           aaaGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACgg                                                                              >  2:96456/1‑140 (MQ=255)                                                           aaaGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACgg                                                                              >  1:1457530/1‑140 (MQ=255)                                                                 tGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCt                                                                        >  2:1250869/1‑140 (MQ=255)                                                                 tGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCt                                                                        >  1:1099363/1‑140 (MQ=255)                                                                 tGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCt                                                                        >  1:1622818/1‑140 (MQ=255)                                                                 tGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGTTTTACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCt                                                                        <  2:1089365/140‑1 (MQ=255)                                                                    ggggTGGGGTGTTCGGAGTGGAACGACATCCGTGATGAGGTGGGGCACTGCGGGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     <  2:784710/138‑1 (MQ=255)                                                                    cggggggCGTGTTCGGAGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     <  2:411317/135‑1 (MQ=255)                                                                    cAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     >  1:126445/1‑140 (MQ=255)                                                                    cAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     >  2:528863/1‑140 (MQ=255)                                                                    cAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     >  2:1636087/1‑140 (MQ=255)                                                                    cAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGa                                                                     >  1:10582/1‑140 (MQ=255)                                                                     aGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAg                                                                    <  1:1250869/140‑1 (MQ=255)                                                                        tGGGGTGTTGGGAGTGGAACGACATCCGTGATGAAGGGGGGCACGGCGGGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTgg                                                                 <  2:701057/140‑1 (MQ=255)                                                                          gCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGTCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCt                                                               >  2:1518216/1‑140 (MQ=255)                                                                           cGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGGTg                                                              >  2:191738/1‑140 (MQ=255)                                                                           cGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTg                                                              >  1:784710/1‑140 (MQ=255)                                                                            gtgtTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAA‑CCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGAt                                                            <  2:735830/140‑1 (MQ=255)                                                                             tgtTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGAt                                                            >  1:477928/1‑140 (MQ=255)                                                                                 gggAGTGGAACGACATCGGTGATGAAGGGGGGCCCTGCGTGGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGt                                                        <  2:1099363/139‑1 (MQ=255)                                                                                      tGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGTCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGa                                                   <  1:1518216/140‑1 (MQ=255)                                                                                      tGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGa                                                   <  1:96456/140‑1 (MQ=255)                                                                                      gggAACGACATCGGTGATGAAGTGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGa                                                   <  2:441809/139‑1 (MQ=255)                                                                                      gggAACGACATCCGTGATGAAGGGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGa                                                   <  2:1629332/139‑1 (MQ=255)                                                                                        gAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACg                                                 <  1:528863/140‑1 (MQ=255)                                                                                        gAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACg                                                 <  1:1636087/140‑1 (MQ=255)                                                                                         aaCGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACgg                                                >  1:627736/1‑140 (MQ=255)                                                                                          aCGACACCCGTGATGAGGGGGGGCACTGCGGGGGGTTTTACAATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGc                                               <  2:286704/140‑1 (MQ=255)                                                                                             aCATCCGTGATGAGGTGGGGCCCGGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGt                                            <  2:1472970/140‑1 (MQ=255)                                                                                                tCCGTGATGAAGTGGGGCACTGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATg                                         <  2:1457530/140‑1 (MQ=255)                                                                                                tCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATg                                         <  1:151703/140‑1 (MQ=255)                                                                                                tCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATg                                         >  1:1270071/1‑140 (MQ=255)                                                                                                cccGTGATGAAGGGGGGCACTGCGTGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATg                                         <  1:1218178/139‑1 (MQ=255)                                                                                                agggTGATGAGGGGGGCCACGGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATg                                         <  2:1090235/137‑1 (MQ=255)                                                                                                  cGTGATGAAGGGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCa                                       <  2:1622818/140‑1 (MQ=255)                                                                                                    tgatgaAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACt                                     <  1:1568766/140‑1 (MQ=255)                                                                                                        gaAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGa                                 >  1:198405/1‑140 (MQ=255)                                                                                                        gaAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGa                                 >  1:230094/1‑140 (MQ=255)                                                                                                              gggCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGactg                           >  1:1090235/1‑137 (MQ=255)                                                                                                               ggCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATccc                          >  2:740957/1‑140 (MQ=255)                                                                                                                 cACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCgg                        >  1:456738/1‑140 (MQ=255)                                                                                                                 cACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCgg                        <  1:1329687/140‑1 (MQ=255)                                                                                                                 aaCTGCGGCGGGTTTACCCATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCgg                        <  2:1281522/139‑1 (MQ=37)                                                                                                                   cTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCa                                       <  1:1783568/123‑1 (MQ=255)                                                                                                                   cTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCa                                       >  2:1783568/1‑123 (MQ=255)                                                                                                                      cGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTAGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGcc                   >  2:1564001/1‑140 (MQ=255)                                                                                                                       gTCGGGTTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCt                  <  1:1564001/140‑1 (MQ=255)                                                                                                                           gggTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAg              <  2:477928/140‑1 (MQ=255)                                                                                                                                tACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTg         >  1:355553/1‑140 (MQ=255)                                                                                                                                       aCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTct  >  1:339483/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                            GCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCT  >  NC_000913/1550529‑1550802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑