Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA41.5% 43.8 / 24.1 41intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (24/0);  new base A (0/17);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 6.60e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCT  >  NC_000913/1824922‑1825185
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tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTATGAGAAGagaaaag                                                                                                                              >  1:1681838/1‑140 (MQ=255)
      aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                        >  1:1557015/1‑140 (MQ=255)
             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                 >  1:921277/1‑140 (MQ=255)
             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                 >  1:65080/1‑140 (MQ=255)
             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                 >  1:1797719/1‑140 (MQ=255)
                  tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                            >  1:1796336/1‑140 (MQ=255)
                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa                                                                                                          >  1:657610/1‑140 (MQ=255)
                    ccccccGGCCCTTTTTTTTCTTTTTTATCGTTCAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGATTTACTTTGGGGGTGATCGGGTTTTTTTTAAACTTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                          <  2:1797719/137‑1 (MQ=255)
                    ccccccGGCCCTTTCTTTTAATTGTTGTCGTTCAGGAACCCGTGGGTGTTTATCGGCAACTGGGGTGATTTACGTGAGTGGTGTTCGGGCTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                          <  2:921277/137‑1 (MQ=255)
                    ccccccGCCCCTTTTTTTTCTTTTTTATGTTTCAGTTACCCGGGGTTGTTTATCGGCACCTGGGGTATTTTACGTTAGTGGTGATCGGGTTTTTTTTACAGCTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                          <  2:1681838/137‑1 (MQ=255)
                         cGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCa                                                                                                     >  1:189425/1‑140 (MQ=255)
                          ggCCCTTTTTTTTCATTGTTATCGTTCAGGTAACGGTGGATGTTTATCGGCAACTGGGGTGTTTTACGTTGGGGGTGATCAGCTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                    <  2:657610/140‑1 (MQ=255)
                          gccccTTTTTTTTCATTGTTATCTTTCAGGTACCGGTGGTTGGTTATCGCCACCTGGGGTATTTTACTTTAGTGGTGATAGGGTTTTTTTTACATTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                    <  2:65080/138‑1 (MQ=255)
                               ttttttttCTTTTTTATCGTTCAGGTACCGGGGGATGGTTATCGGCACCTGGGGTGTTTTAGGTTAGTGGGGATCAGTTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                               <  2:1796336/136‑1 (MQ=255)
                                 ttttttCTTTTTTATCGTTCGGGTAACGGGGGTTGTTTACCGACAACTGGGGTGATTTACTTTGGGGGTGATCGGGTTTTTTTTAAACCTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                             <  2:189425/138‑1 (MQ=255)
                                    tttGATTTTTTTCGTTCAGGTATCCGTGGATGGTTAGCGGAATTTCGGGTGATCTTTTTTTGGGGTAATCAGGTTTTTTTTACAGTTGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                          <  2:762717/140‑1 (MQ=255)
                                             tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                 >  1:171674/1‑140 (MQ=255)
                                             tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                 >  2:1680854/1‑140 (MQ=255)
                                                    gaGGTAACGGTGGATGGTTACCGGCACCTGGGGTATTTTACGTTAGTGGTGATCAGGTTTTTTTAACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                          <  2:171674/139‑1 (MQ=255)
                                                    cAGTTATCCGTGGATGGTTATCGGCAACTGGGGTGATTTAGGTTAGGGGGTTTGGGGTTTTTTTTACAATTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                          <  2:567342/140‑1 (MQ=255)
                                                     gggTAACGGGGGTTGTTTACGGCCAACTGGGTTTTTTTAGGTTAGTGGTGATCGGGTTTTTTTAACAATTTTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                         <  2:911250/139‑1 (MQ=255)
                                                      ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                        >  1:1503627/1‑140 (MQ=255)
                                                       gTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                       >  1:1649175/1‑140 (MQ=255)
                                                              tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                >  1:482237/1‑140 (MQ=255)
                                                                 aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                             >  1:1232188/1‑140 (MQ=255)
                                                                    tttAGCGCCACCTGGGGTGATTTCGGTTAGTGGTGATCGGGTTTTTTTTACAATTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                          <  2:1649175/139‑1 (MQ=255)
                                                                     ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg                                                         >  1:1566888/1‑140 (MQ=255)
                                                                       ttCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                       <  2:482237/139‑1 (MQ=255)
                                                                               ctggggTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                               <  2:1566888/137‑1 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                               >  2:700032/1‑140 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                               >  1:1767156/1‑140 (MQ=255)
                                                                                      tttttACGTTGGTGGTGATCGGGTTTTTTTTACATCTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                        <  2:1767156/138‑1 (MQ=255)
                                                                                      aaTTTACTTTGGTGGTGATCGGTTTTTTTTTACAATTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                        <  2:1347896/139‑1 (MQ=255)
                                                                                              ttAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCa                                >  2:1277777/1‑140 (MQ=255)
                                                                                               tggtggtgATCAGGTTTTTTTAAAAATTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                               <  2:370305/138‑1 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGcc                            >  1:1347896/1‑139 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                            >  1:40633/1‑140 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                            >  1:370305/1‑140 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTTTTTTTGCTGAAAAATTATTTAGCAGTTGCAGGCa                            >  2:389627/1‑140 (MQ=255)
                                                                                                    ttgATCAGTTTTTTTTTACAACTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtt                          <  2:1144589/139‑1 (MQ=255)
                                                                                                                    tacacgtGTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg          <  1:389627/134‑1 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa        >  2:215386/1‑134 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt  >  1:1765281/1‑140 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt  >  1:1531535/1‑140 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa        <  1:215386/134‑1 (MQ=255)
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TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCT  >  NC_000913/1824922‑1825185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑